Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1

Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findin...

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Main Authors: Rosani, Umberto, Abbadi, Miriam, Green, Timothy, Bai, Chang-Ming, Turolla, Edoardo, Arcangeli, Giuseppe, K. Mathias Wegner, Venier, Paola
Format: Article in Journal/Newspaper
Language:unknown
Published: figshare 2020
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Online Access:https://dx.doi.org/10.6084/m9.figshare.c.5117995
https://springernature.figshare.com/collections/Parallel_analysis_of_miRNAs_and_mRNAs_suggests_distinct_regulatory_networks_in_Crassostrea_gigas_infected_by_Ostreid_herpesvirus_1/5117995
id ftdatacite:10.6084/m9.figshare.c.5117995
record_format openpolar
spelling ftdatacite:10.6084/m9.figshare.c.5117995 2023-05-15T15:58:16+02:00 Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1 Rosani, Umberto Abbadi, Miriam Green, Timothy Bai, Chang-Ming Turolla, Edoardo Arcangeli, Giuseppe K. Mathias Wegner Venier, Paola 2020 https://dx.doi.org/10.6084/m9.figshare.c.5117995 https://springernature.figshare.com/collections/Parallel_analysis_of_miRNAs_and_mRNAs_suggests_distinct_regulatory_networks_in_Crassostrea_gigas_infected_by_Ostreid_herpesvirus_1/5117995 unknown figshare https://dx.doi.org/10.1186/s12864-020-07026-7 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Genetics FOS Biological sciences Collection article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.c.5117995 https://doi.org/10.1186/s12864-020-07026-7 2021-11-05T12:55:41Z Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection in C. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks. Article in Journal/Newspaper Crassostrea gigas DataCite Metadata Store (German National Library of Science and Technology)
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language unknown
topic Genetics
FOS Biological sciences
spellingShingle Genetics
FOS Biological sciences
Rosani, Umberto
Abbadi, Miriam
Green, Timothy
Bai, Chang-Ming
Turolla, Edoardo
Arcangeli, Giuseppe
K. Mathias Wegner
Venier, Paola
Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
topic_facet Genetics
FOS Biological sciences
description Abstract Background Since 2008, the aquaculture production of Crassostrea gigas was heavily affected by mass mortalities associated to Ostreid herpesvirus 1 (OsHV-1) microvariants worldwide. Transcriptomic studies revealed the major antiviral pathways of the oyster immune response while other findings suggested that also small non-coding RNAs (sncRNA) such as microRNAs might act as key regulators of the oyster response against OsHV-1. To explore the explicit connection between small non-coding and protein-coding transcripts, we performed paired whole transcriptome analysis of sncRNA and messenger RNA (mRNA) in six oysters selected for different intensities of OsHV-1 infection. Results The mRNA profiles of the naturally infected oysters were mostly governed by the transcriptional activity of OsHV-1, with several differentially expressed genes mapping to the interferon, toll, apoptosis, and pro-PO pathways. In contrast, miRNA profiles suggested more complex regulatory mechanisms, with 15 differentially expressed miRNAs (DE-miRNA) pointing to a possible modulation of the host response during OsHV-1 infection. We predicted 68 interactions between DE-miRNAs and oyster 3′-UTRs, but only few of them involved antiviral genes. The sncRNA reads assigned to OsHV-1 rather resembled mRNA degradation products, suggesting the absence of genuine viral miRNAs. Conclusions We provided data describing the miRNAome during OsHV-1 infection in C. gigas. This information can be used to understand the role of miRNAs in healthy and diseased oysters, to identify new targets for functional studies and, eventually to disentangle cause and effect relationships during viral infections in marine mollusks.
format Article in Journal/Newspaper
author Rosani, Umberto
Abbadi, Miriam
Green, Timothy
Bai, Chang-Ming
Turolla, Edoardo
Arcangeli, Giuseppe
K. Mathias Wegner
Venier, Paola
author_facet Rosani, Umberto
Abbadi, Miriam
Green, Timothy
Bai, Chang-Ming
Turolla, Edoardo
Arcangeli, Giuseppe
K. Mathias Wegner
Venier, Paola
author_sort Rosani, Umberto
title Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
title_short Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
title_full Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
title_fullStr Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
title_full_unstemmed Parallel analysis of miRNAs and mRNAs suggests distinct regulatory networks in Crassostrea gigas infected by Ostreid herpesvirus 1
title_sort parallel analysis of mirnas and mrnas suggests distinct regulatory networks in crassostrea gigas infected by ostreid herpesvirus 1
publisher figshare
publishDate 2020
url https://dx.doi.org/10.6084/m9.figshare.c.5117995
https://springernature.figshare.com/collections/Parallel_analysis_of_miRNAs_and_mRNAs_suggests_distinct_regulatory_networks_in_Crassostrea_gigas_infected_by_Ostreid_herpesvirus_1/5117995
genre Crassostrea gigas
genre_facet Crassostrea gigas
op_relation https://dx.doi.org/10.1186/s12864-020-07026-7
op_rights Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
cc-by-4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.6084/m9.figshare.c.5117995
https://doi.org/10.1186/s12864-020-07026-7
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