Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris ...
Abstract Background Glycosylphosphatidylinositol (GPI)-anchored glycoproteins have diverse intrinsic functions in yeasts, and they also have different uses in vitro. In this study, the functions of potential GPI proteins in Pichia pastoris were explored by gene knockout approaches. Results Through a...
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ftdatacite:10.6084/m9.figshare.c.5100959.v1 2024-04-28T08:02:30+00:00 Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris ... Wang, Pan Lin, Ying Zou, Chengjuan Zhao, Fengguang Liang, Shuli Zheng, Suiping Han, Shuangyan 2020 https://dx.doi.org/10.6084/m9.figshare.c.5100959.v1 https://springernature.figshare.com/collections/Construction_and_screening_of_a_glycosylphosphatidylinositol_protein_deletion_library_in_Pichia_pastoris/5100959/1 unknown figshare https://dx.doi.org/10.6084/m9.figshare.c.5100959 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Biophysics Biochemistry Genetics FOS Biological sciences Biotechnology Ecology Cancer Plant Biology Collection article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.c.5100959.v110.6084/m9.figshare.c.5100959 2024-04-02T12:00:32Z Abstract Background Glycosylphosphatidylinositol (GPI)-anchored glycoproteins have diverse intrinsic functions in yeasts, and they also have different uses in vitro. In this study, the functions of potential GPI proteins in Pichia pastoris were explored by gene knockout approaches. Results Through an extensive knockout of GPI proteins in P. pastoris, a single-gene deletion library was constructed for 45 predicted GPI proteins. The knockout of proteins may lead to the activation of a cellular response named the ‘compensatory mechanism’, which is characterized by changes in the content and relationship between cell wall polysaccharides and surface proteins. Among the 45 deletion strains, five showed obvious methanol tolerance, four owned high content of cell wall polysaccharides, and four had a high surface hydrophobicity. Some advantages of these strains as production hosts were revealed. Furthermore, the deletion strains with high surface hydrophobicity were used as hosts to display Candida antarctica lipase ... Article in Journal/Newspaper Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
Biophysics Biochemistry Genetics FOS Biological sciences Biotechnology Ecology Cancer Plant Biology |
spellingShingle |
Biophysics Biochemistry Genetics FOS Biological sciences Biotechnology Ecology Cancer Plant Biology Wang, Pan Lin, Ying Zou, Chengjuan Zhao, Fengguang Liang, Shuli Zheng, Suiping Han, Shuangyan Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris ... |
topic_facet |
Biophysics Biochemistry Genetics FOS Biological sciences Biotechnology Ecology Cancer Plant Biology |
description |
Abstract Background Glycosylphosphatidylinositol (GPI)-anchored glycoproteins have diverse intrinsic functions in yeasts, and they also have different uses in vitro. In this study, the functions of potential GPI proteins in Pichia pastoris were explored by gene knockout approaches. Results Through an extensive knockout of GPI proteins in P. pastoris, a single-gene deletion library was constructed for 45 predicted GPI proteins. The knockout of proteins may lead to the activation of a cellular response named the ‘compensatory mechanism’, which is characterized by changes in the content and relationship between cell wall polysaccharides and surface proteins. Among the 45 deletion strains, five showed obvious methanol tolerance, four owned high content of cell wall polysaccharides, and four had a high surface hydrophobicity. Some advantages of these strains as production hosts were revealed. Furthermore, the deletion strains with high surface hydrophobicity were used as hosts to display Candida antarctica lipase ... |
format |
Article in Journal/Newspaper |
author |
Wang, Pan Lin, Ying Zou, Chengjuan Zhao, Fengguang Liang, Shuli Zheng, Suiping Han, Shuangyan |
author_facet |
Wang, Pan Lin, Ying Zou, Chengjuan Zhao, Fengguang Liang, Shuli Zheng, Suiping Han, Shuangyan |
author_sort |
Wang, Pan |
title |
Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris ... |
title_short |
Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris ... |
title_full |
Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris ... |
title_fullStr |
Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris ... |
title_full_unstemmed |
Construction and screening of a glycosylphosphatidylinositol protein deletion library in Pichia pastoris ... |
title_sort |
construction and screening of a glycosylphosphatidylinositol protein deletion library in pichia pastoris ... |
publisher |
figshare |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.5100959.v1 https://springernature.figshare.com/collections/Construction_and_screening_of_a_glycosylphosphatidylinositol_protein_deletion_library_in_Pichia_pastoris/5100959/1 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://dx.doi.org/10.6084/m9.figshare.c.5100959 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.c.5100959.v110.6084/m9.figshare.c.5100959 |
_version_ |
1797573819963015168 |