Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines
Abstract Background The 13-valent Pneumococcal Conjugate Vaccine (PCV-13) was introduced in the National Immunization Programme (NIP) schedule in Russia in March 2014. Previously, the 7-valent Pneumococcal Conjugate Vaccine (PCV-7) was marketed in Russia in 2009 but has never been offered for mass v...
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ftdatacite:10.6084/m9.figshare.c.4939974 2023-05-15T15:23:44+02:00 Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines V. Vorobieva S. Jensen A-S Furberg H-C Slotved T. Bazhukova B. Haldorsen D. A. Caugant A. Sundsfjord P. Valentiner-Branth G. S. Simonsen 2020 https://dx.doi.org/10.6084/m9.figshare.c.4939974 https://springernature.figshare.com/collections/Epidemiological_and_molecular_characterization_of_Streptococcus_pneumoniae_carriage_strains_in_pre-school_children_in_Arkhangelsk_northern_European_Russia_prior_to_the_introduction_of_conjugate_pneumococcal_vaccines/4939974 unknown figshare https://dx.doi.org/10.1186/s12879-020-04998-5 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Medicine Microbiology FOS Biological sciences Molecular Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Sociology FOS Sociology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer 110309 Infectious Diseases FOS Health sciences Collection article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.c.4939974 https://doi.org/10.1186/s12879-020-04998-5 2021-11-05T12:55:41Z Abstract Background The 13-valent Pneumococcal Conjugate Vaccine (PCV-13) was introduced in the National Immunization Programme (NIP) schedule in Russia in March 2014. Previously, the 7-valent Pneumococcal Conjugate Vaccine (PCV-7) was marketed in Russia in 2009 but has never been offered for mass vaccination. A carriage study was performed among children in Arkhangelsk in 2006. The objective was to determine the prevalence of carriage, serotype distribution, antimicrobial susceptibility and the molecular structure of Streptococcus pneumoniae strains before marketing and introduction of PCV-13. Methods A cross-sectional study was conducted on a cluster-randomized sample of children and a self-administrated questionnaire for parents/guardians. Nasopharyngeal samples were collected from 438 children younger than 7 years attending nurseries and kindergartens in the Arkhangelsk region, Russia. Detailed demographic data, as well as information about the child’s health, traveling, exposure to antimicrobials within the last 3 months and anthropometric measurements were collected for all study subjects. Variables extracted from the questionnaire were analysed using statistic regression models to estimate the risk of carriage. All pneumococcal isolates were examined with susceptibility testing, serotyping and multilocus sequence typing. Results The overall prevalence of asymptomatic carriage was high and peaking at 36 months with a rate of 57%. PCV-13 covered 67.3% of the detected strains. High rates of non-susceptibility to penicillin, macrolides and multidrug resistance were associated with specific vaccine serotypes, pandemic clones, and local sequence types. Nine percent of isolates represented three globally disseminated disease-associated pandemic clones; penicillin- and macrolide-resistant clones NorwayNT-42 and Poland6B-20, as well as penicillin- and macrolide-susceptible clone Netherlands3-31. A high level of antimicrobial consumption was noted by the study. According to the parent’s reports, 89.5% of the children used at least one antimicrobial regime since birth. None of the hypothesised predictors of S. pneumoniae carriage were statistically significant in univariable and multivariable logistic models. Conclusions The study identified a high coverage of the PCV-13-vaccine, but serotype replacement and expansion of globally disseminated disease-associated clones with non-vaccine serotypes may be expected. Further surveillance of antimicrobial resistance and serotype distribution is therefore required. Article in Journal/Newspaper Arkhangelsk DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Medicine Microbiology FOS Biological sciences Molecular Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Sociology FOS Sociology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer 110309 Infectious Diseases FOS Health sciences |
spellingShingle |
Medicine Microbiology FOS Biological sciences Molecular Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Sociology FOS Sociology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer 110309 Infectious Diseases FOS Health sciences V. Vorobieva S. Jensen A-S Furberg H-C Slotved T. Bazhukova B. Haldorsen D. A. Caugant A. Sundsfjord P. Valentiner-Branth G. S. Simonsen Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines |
topic_facet |
Medicine Microbiology FOS Biological sciences Molecular Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Sociology FOS Sociology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer 110309 Infectious Diseases FOS Health sciences |
description |
Abstract Background The 13-valent Pneumococcal Conjugate Vaccine (PCV-13) was introduced in the National Immunization Programme (NIP) schedule in Russia in March 2014. Previously, the 7-valent Pneumococcal Conjugate Vaccine (PCV-7) was marketed in Russia in 2009 but has never been offered for mass vaccination. A carriage study was performed among children in Arkhangelsk in 2006. The objective was to determine the prevalence of carriage, serotype distribution, antimicrobial susceptibility and the molecular structure of Streptococcus pneumoniae strains before marketing and introduction of PCV-13. Methods A cross-sectional study was conducted on a cluster-randomized sample of children and a self-administrated questionnaire for parents/guardians. Nasopharyngeal samples were collected from 438 children younger than 7 years attending nurseries and kindergartens in the Arkhangelsk region, Russia. Detailed demographic data, as well as information about the child’s health, traveling, exposure to antimicrobials within the last 3 months and anthropometric measurements were collected for all study subjects. Variables extracted from the questionnaire were analysed using statistic regression models to estimate the risk of carriage. All pneumococcal isolates were examined with susceptibility testing, serotyping and multilocus sequence typing. Results The overall prevalence of asymptomatic carriage was high and peaking at 36 months with a rate of 57%. PCV-13 covered 67.3% of the detected strains. High rates of non-susceptibility to penicillin, macrolides and multidrug resistance were associated with specific vaccine serotypes, pandemic clones, and local sequence types. Nine percent of isolates represented three globally disseminated disease-associated pandemic clones; penicillin- and macrolide-resistant clones NorwayNT-42 and Poland6B-20, as well as penicillin- and macrolide-susceptible clone Netherlands3-31. A high level of antimicrobial consumption was noted by the study. According to the parent’s reports, 89.5% of the children used at least one antimicrobial regime since birth. None of the hypothesised predictors of S. pneumoniae carriage were statistically significant in univariable and multivariable logistic models. Conclusions The study identified a high coverage of the PCV-13-vaccine, but serotype replacement and expansion of globally disseminated disease-associated clones with non-vaccine serotypes may be expected. Further surveillance of antimicrobial resistance and serotype distribution is therefore required. |
format |
Article in Journal/Newspaper |
author |
V. Vorobieva S. Jensen A-S Furberg H-C Slotved T. Bazhukova B. Haldorsen D. A. Caugant A. Sundsfjord P. Valentiner-Branth G. S. Simonsen |
author_facet |
V. Vorobieva S. Jensen A-S Furberg H-C Slotved T. Bazhukova B. Haldorsen D. A. Caugant A. Sundsfjord P. Valentiner-Branth G. S. Simonsen |
author_sort |
V. Vorobieva S. Jensen |
title |
Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines |
title_short |
Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines |
title_full |
Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines |
title_fullStr |
Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines |
title_full_unstemmed |
Epidemiological and molecular characterization of Streptococcus pneumoniae carriage strains in pre-school children in Arkhangelsk, northern European Russia, prior to the introduction of conjugate pneumococcal vaccines |
title_sort |
epidemiological and molecular characterization of streptococcus pneumoniae carriage strains in pre-school children in arkhangelsk, northern european russia, prior to the introduction of conjugate pneumococcal vaccines |
publisher |
figshare |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4939974 https://springernature.figshare.com/collections/Epidemiological_and_molecular_characterization_of_Streptococcus_pneumoniae_carriage_strains_in_pre-school_children_in_Arkhangelsk_northern_European_Russia_prior_to_the_introduction_of_conjugate_pneumococcal_vaccines/4939974 |
genre |
Arkhangelsk |
genre_facet |
Arkhangelsk |
op_relation |
https://dx.doi.org/10.1186/s12879-020-04998-5 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4939974 https://doi.org/10.1186/s12879-020-04998-5 |
_version_ |
1766354369182171136 |