Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered european eel
Abstract Background Once highly abundant, the European eel (Anguilla anguilla L.; Anguillidae; Teleostei) is considered to be critically endangered and on the verge of extinction, as the stock has declined by 90-99% since the 1980s. Yet, the species is poorly characterized at molecular level with li...
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ftdatacite:10.6084/m9.figshare.c.4872897 2023-05-15T13:27:15+02:00 Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered european eel Coppe, Alessandro Pujolar, Jose Martin Maes, Gregory E Larsen, Peter F Hansen, Michael M Bernatchez, Louis Zane, Lorenzo Bortoluzzi, Stefania 2021 https://dx.doi.org/10.6084/m9.figshare.c.4872897 https://springernature.figshare.com/collections/Sequencing_de_novo_annotation_and_analysis_of_the_first_Anguilla_anguilla_transcriptome_EeelBase_opens_new_perspectives_for_the_study_of_the_critically_endangered_european_eel/4872897 unknown figshare https://dx.doi.org/10.1186/1471-2164-11-635 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Genetics FOS Biological sciences Collection article 2021 ftdatacite https://doi.org/10.6084/m9.figshare.c.4872897 https://doi.org/10.1186/1471-2164-11-635 2021-11-05T12:55:41Z Abstract Background Once highly abundant, the European eel (Anguilla anguilla L.; Anguillidae; Teleostei) is considered to be critically endangered and on the verge of extinction, as the stock has declined by 90-99% since the 1980s. Yet, the species is poorly characterized at molecular level with little sequence information available in public databases. Results The first European eel transcriptome was obtained by 454 FLX Titanium sequencing of a normalized cDNA library, produced from a pool of 18 glass eels (juveniles) from the French Atlantic coast and two sites in the Mediterranean coast. Over 310,000 reads were assembled in a total of 19,631 transcribed contigs, with an average length of 531 nucleotides. Overall 36% of the contigs were annotated to known protein/nucleotide sequences and 35 putative miRNA identified. Conclusions This study represents the first transcriptome analysis for a critically endangered species. EeelBase, a dedicated database of annotated transcriptome sequences of the European eel is freely available at http://compgen.bio.unipd.it/eeelbase . Considering the multiple factors potentially involved in the decline of the European eel, including anthropogenic factors such as pollution and human-introduced diseases, our results will provide a rich source of data to discover and identify new genes, characterize gene expression, as well as for identification of genetic markers scattered across the genome to be used in various applications. Article in Journal/Newspaper Anguilla anguilla European eel DataCite Metadata Store (German National Library of Science and Technology) |
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collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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language |
unknown |
topic |
Genetics FOS Biological sciences |
spellingShingle |
Genetics FOS Biological sciences Coppe, Alessandro Pujolar, Jose Martin Maes, Gregory E Larsen, Peter F Hansen, Michael M Bernatchez, Louis Zane, Lorenzo Bortoluzzi, Stefania Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered european eel |
topic_facet |
Genetics FOS Biological sciences |
description |
Abstract Background Once highly abundant, the European eel (Anguilla anguilla L.; Anguillidae; Teleostei) is considered to be critically endangered and on the verge of extinction, as the stock has declined by 90-99% since the 1980s. Yet, the species is poorly characterized at molecular level with little sequence information available in public databases. Results The first European eel transcriptome was obtained by 454 FLX Titanium sequencing of a normalized cDNA library, produced from a pool of 18 glass eels (juveniles) from the French Atlantic coast and two sites in the Mediterranean coast. Over 310,000 reads were assembled in a total of 19,631 transcribed contigs, with an average length of 531 nucleotides. Overall 36% of the contigs were annotated to known protein/nucleotide sequences and 35 putative miRNA identified. Conclusions This study represents the first transcriptome analysis for a critically endangered species. EeelBase, a dedicated database of annotated transcriptome sequences of the European eel is freely available at http://compgen.bio.unipd.it/eeelbase . Considering the multiple factors potentially involved in the decline of the European eel, including anthropogenic factors such as pollution and human-introduced diseases, our results will provide a rich source of data to discover and identify new genes, characterize gene expression, as well as for identification of genetic markers scattered across the genome to be used in various applications. |
format |
Article in Journal/Newspaper |
author |
Coppe, Alessandro Pujolar, Jose Martin Maes, Gregory E Larsen, Peter F Hansen, Michael M Bernatchez, Louis Zane, Lorenzo Bortoluzzi, Stefania |
author_facet |
Coppe, Alessandro Pujolar, Jose Martin Maes, Gregory E Larsen, Peter F Hansen, Michael M Bernatchez, Louis Zane, Lorenzo Bortoluzzi, Stefania |
author_sort |
Coppe, Alessandro |
title |
Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered european eel |
title_short |
Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered european eel |
title_full |
Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered european eel |
title_fullStr |
Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered european eel |
title_full_unstemmed |
Sequencing, de novo annotation and analysis of the first Anguilla anguilla transcriptome: EeelBase opens new perspectives for the study of the critically endangered european eel |
title_sort |
sequencing, de novo annotation and analysis of the first anguilla anguilla transcriptome: eeelbase opens new perspectives for the study of the critically endangered european eel |
publisher |
figshare |
publishDate |
2021 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4872897 https://springernature.figshare.com/collections/Sequencing_de_novo_annotation_and_analysis_of_the_first_Anguilla_anguilla_transcriptome_EeelBase_opens_new_perspectives_for_the_study_of_the_critically_endangered_european_eel/4872897 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_relation |
https://dx.doi.org/10.1186/1471-2164-11-635 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4872897 https://doi.org/10.1186/1471-2164-11-635 |
_version_ |
1766397285086789632 |