Supplementary material from "Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress"
The intestinal epithelium is a selectively permeable barrier for nutrients, electrolytes and water, while maintaining an effective protection against pathogens. Combinations of stressors throughout an animal's life, especially in agriculture and aquaculture settings may affect the regular opera...
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ftdatacite:10.6084/m9.figshare.c.4860600 2023-05-15T15:31:04+02:00 Supplementary material from "Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress" Løvmo, Signe Dille Madaro, Angelico Whatmore, Paul Bardal, Tora Mari-Ann Ostensen Sandve, Simen R. Olsen, Rolf Erik 2020 https://dx.doi.org/10.6084/m9.figshare.c.4860600 https://rs.figshare.com/collections/Supplementary_material_from_Mid_and_hindgut_transcriptome_profiling_analysis_of_Atlantic_salmon_i_Salmon_salar_i_under_unpredictable_chronic_stress_/4860600 unknown The Royal Society https://dx.doi.org/10.1098/rsos.191480 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Molecular Biology Physiology FOS Biological sciences Collection article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.c.4860600 https://doi.org/10.1098/rsos.191480 2021-11-05T12:55:41Z The intestinal epithelium is a selectively permeable barrier for nutrients, electrolytes and water, while maintaining an effective protection against pathogens. Combinations of stressors throughout an animal's life, especially in agriculture and aquaculture settings may affect the regular operativity of this organ with negative consequences for animal welfare. In the current study, we report the effects of a three-week unpredictable chronic stress (UCS) period on the intestinal morphology and transcriptome response of Atlantic salmon ( Salmo salar ) parr midgut and hindgut. Midgut and hindgut from both control and UCS fish were collected for histology and RNA-sequencing analysis to identify respective changes in the membrane structures and putative genes and pathways responding to UCS. Histological analysis did not show any significant effect on morphometric parameters. In the midgut, 1030 genes were differentially expressed following UCS, resulting in 279 genes which were involved in 13 metabolic pathways, including tissue repair pathways. In the hindgut, following UCS, 591 differentially expressed genes were detected with 426 downregulated and 165 upregulated. A total of 53 genes were related to three pathways. Downregulated genes include cellular senescence pathways, p53 signalling and cytokine–cytokine receptor pathways. The overall results corroborate that salmon parr were at least partly habituating to the UCS treatment. In midgut, the main upregulation was related to cell growth and repair, while in the hindgut there were indications of the activated apoptotic pathway, reduced cell repair and inhibited immune/anti-inflammatory capacity. This may be the trade-off between habituating to UCS and health resilience. This study suggests possible integrated genetic regulatory mechanisms that are tuned when farmed Atlantic salmon parr attempt to cope with UCS. Article in Journal/Newspaper Atlantic salmon Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
Molecular Biology Physiology FOS Biological sciences |
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Molecular Biology Physiology FOS Biological sciences Løvmo, Signe Dille Madaro, Angelico Whatmore, Paul Bardal, Tora Mari-Ann Ostensen Sandve, Simen R. Olsen, Rolf Erik Supplementary material from "Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress" |
topic_facet |
Molecular Biology Physiology FOS Biological sciences |
description |
The intestinal epithelium is a selectively permeable barrier for nutrients, electrolytes and water, while maintaining an effective protection against pathogens. Combinations of stressors throughout an animal's life, especially in agriculture and aquaculture settings may affect the regular operativity of this organ with negative consequences for animal welfare. In the current study, we report the effects of a three-week unpredictable chronic stress (UCS) period on the intestinal morphology and transcriptome response of Atlantic salmon ( Salmo salar ) parr midgut and hindgut. Midgut and hindgut from both control and UCS fish were collected for histology and RNA-sequencing analysis to identify respective changes in the membrane structures and putative genes and pathways responding to UCS. Histological analysis did not show any significant effect on morphometric parameters. In the midgut, 1030 genes were differentially expressed following UCS, resulting in 279 genes which were involved in 13 metabolic pathways, including tissue repair pathways. In the hindgut, following UCS, 591 differentially expressed genes were detected with 426 downregulated and 165 upregulated. A total of 53 genes were related to three pathways. Downregulated genes include cellular senescence pathways, p53 signalling and cytokine–cytokine receptor pathways. The overall results corroborate that salmon parr were at least partly habituating to the UCS treatment. In midgut, the main upregulation was related to cell growth and repair, while in the hindgut there were indications of the activated apoptotic pathway, reduced cell repair and inhibited immune/anti-inflammatory capacity. This may be the trade-off between habituating to UCS and health resilience. This study suggests possible integrated genetic regulatory mechanisms that are tuned when farmed Atlantic salmon parr attempt to cope with UCS. |
format |
Article in Journal/Newspaper |
author |
Løvmo, Signe Dille Madaro, Angelico Whatmore, Paul Bardal, Tora Mari-Ann Ostensen Sandve, Simen R. Olsen, Rolf Erik |
author_facet |
Løvmo, Signe Dille Madaro, Angelico Whatmore, Paul Bardal, Tora Mari-Ann Ostensen Sandve, Simen R. Olsen, Rolf Erik |
author_sort |
Løvmo, Signe Dille |
title |
Supplementary material from "Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress" |
title_short |
Supplementary material from "Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress" |
title_full |
Supplementary material from "Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress" |
title_fullStr |
Supplementary material from "Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress" |
title_full_unstemmed |
Supplementary material from "Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress" |
title_sort |
supplementary material from "mid and hindgut transcriptome profiling analysis of atlantic salmon ( salmon salar ) under unpredictable chronic stress" |
publisher |
The Royal Society |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4860600 https://rs.figshare.com/collections/Supplementary_material_from_Mid_and_hindgut_transcriptome_profiling_analysis_of_Atlantic_salmon_i_Salmon_salar_i_under_unpredictable_chronic_stress_/4860600 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://dx.doi.org/10.1098/rsos.191480 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4860600 https://doi.org/10.1098/rsos.191480 |
_version_ |
1766361560120295424 |