Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site
Abstract Background A virus was isolated from diseased turbot Scophthalmus maximus in China. Biophysical and biochemical assays, electron microscopy, and genome electrophoresis revealed that the virus belonged to the genus Aquareovirus, and was named Scophthalmus maximus reovirus (SMReV). To the bes...
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ftdatacite:10.6084/m9.figshare.c.4845483 2023-05-15T18:15:48+02:00 Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site Ke, Fei He, Li-Bo Pei, Chao Zhang, Qi-Ya 2020 https://dx.doi.org/10.6084/m9.figshare.c.4845483 https://springernature.figshare.com/collections/Turbot_reovirus_SMReV_genome_encoding_a_FAST_protein_with_a_non-AUG_start_site/4845483 unknown figshare https://dx.doi.org/10.1186/1471-2164-12-323 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 CC-BY Biochemistry Microbiology FOS Biological sciences Genetics Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences 60506 Virology Collection article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.c.4845483 https://doi.org/10.1186/1471-2164-12-323 2021-11-05T12:55:41Z Abstract Background A virus was isolated from diseased turbot Scophthalmus maximus in China. Biophysical and biochemical assays, electron microscopy, and genome electrophoresis revealed that the virus belonged to the genus Aquareovirus, and was named Scophthalmus maximus reovirus (SMReV). To the best of our knowledge, no complete sequence of an aquareovirus from marine fish has been determined. Therefore, the complete characterization and analysis of the genome of this novel aquareovirus will facilitate further understanding of the taxonomic distribution of aquareovirus species and the molecular mechanism of its pathogenesis. Results The full-length genome sequences of SMReV were determined. It comprises eleven dsRNA segments covering 24,042 base pairs and has the largest S4 genome segment in the sequenced aquareoviruses. Sequence analysis showed that all of the segments contained six conserved nucleotides at the 5' end and five conserved nucleotides at the 3' end (5'-GUUUUA ---- UCAUC-3'). The encoded amino acid sequences share the highest sequence identities with the respective proteins of aquareoviruses in species group Aquareovirus A. Phylogenetic analysis based on the major outer capsid protein VP7 and RNA-dependent RNA polymerase were performed. Members in Aquareovirus were clustered in two groups, one from fresh water fish and the other from marine fish. Furthermore, a fusion associated small transmembrane (FAST) protein NS22, which is translated from a non-AUG start site, was identified in the S7 segment. Conclusions This study has provided the complete genome sequence of a novel isolated aquareovirus from marine fish. Amino acids comparison and phylogenetic analysis suggested that SMReV was a new aquareovirus in the species group Aquareovirus A. Phylogenetic analysis among aquareoviruses revealed that VP7 could be used as a reference to divide the aquareovirus from hosts in fresh water or marine. In addition, a FAST protein with a non-AUG start site was identified, which partially contributed to the cytopathic effect caused by the virus infection. These results provide new insights into the virus-host and virus-environment interactions. Article in Journal/Newspaper Scophthalmus maximus Turbot DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Microbiology FOS Biological sciences Genetics Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences 60506 Virology |
spellingShingle |
Biochemistry Microbiology FOS Biological sciences Genetics Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences 60506 Virology Ke, Fei He, Li-Bo Pei, Chao Zhang, Qi-Ya Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site |
topic_facet |
Biochemistry Microbiology FOS Biological sciences Genetics Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences 60506 Virology |
description |
Abstract Background A virus was isolated from diseased turbot Scophthalmus maximus in China. Biophysical and biochemical assays, electron microscopy, and genome electrophoresis revealed that the virus belonged to the genus Aquareovirus, and was named Scophthalmus maximus reovirus (SMReV). To the best of our knowledge, no complete sequence of an aquareovirus from marine fish has been determined. Therefore, the complete characterization and analysis of the genome of this novel aquareovirus will facilitate further understanding of the taxonomic distribution of aquareovirus species and the molecular mechanism of its pathogenesis. Results The full-length genome sequences of SMReV were determined. It comprises eleven dsRNA segments covering 24,042 base pairs and has the largest S4 genome segment in the sequenced aquareoviruses. Sequence analysis showed that all of the segments contained six conserved nucleotides at the 5' end and five conserved nucleotides at the 3' end (5'-GUUUUA ---- UCAUC-3'). The encoded amino acid sequences share the highest sequence identities with the respective proteins of aquareoviruses in species group Aquareovirus A. Phylogenetic analysis based on the major outer capsid protein VP7 and RNA-dependent RNA polymerase were performed. Members in Aquareovirus were clustered in two groups, one from fresh water fish and the other from marine fish. Furthermore, a fusion associated small transmembrane (FAST) protein NS22, which is translated from a non-AUG start site, was identified in the S7 segment. Conclusions This study has provided the complete genome sequence of a novel isolated aquareovirus from marine fish. Amino acids comparison and phylogenetic analysis suggested that SMReV was a new aquareovirus in the species group Aquareovirus A. Phylogenetic analysis among aquareoviruses revealed that VP7 could be used as a reference to divide the aquareovirus from hosts in fresh water or marine. In addition, a FAST protein with a non-AUG start site was identified, which partially contributed to the cytopathic effect caused by the virus infection. These results provide new insights into the virus-host and virus-environment interactions. |
format |
Article in Journal/Newspaper |
author |
Ke, Fei He, Li-Bo Pei, Chao Zhang, Qi-Ya |
author_facet |
Ke, Fei He, Li-Bo Pei, Chao Zhang, Qi-Ya |
author_sort |
Ke, Fei |
title |
Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site |
title_short |
Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site |
title_full |
Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site |
title_fullStr |
Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site |
title_full_unstemmed |
Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site |
title_sort |
turbot reovirus (smrev) genome encoding a fast protein with a non-aug start site |
publisher |
figshare |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4845483 https://springernature.figshare.com/collections/Turbot_reovirus_SMReV_genome_encoding_a_FAST_protein_with_a_non-AUG_start_site/4845483 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
https://dx.doi.org/10.1186/1471-2164-12-323 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4845483 https://doi.org/10.1186/1471-2164-12-323 |
_version_ |
1766189021306814464 |