Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon
Abstract Background Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ ce...
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ftdatacite:10.6084/m9.figshare.c.4837938.v1 2023-05-15T15:32:21+02:00 Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon Kleppe, Lene Edvardsen, Rolf Furmanek, Tomasz Andersson, Eva Skaftnesmo, Kai Segafredo, Frida Thyri Wargelius, Anna 2020 https://dx.doi.org/10.6084/m9.figshare.c.4837938.v1 https://springernature.figshare.com/collections/Transcriptomic_analysis_of_dead_end_knockout_testis_reveals_germ_cell_and_gonadal_somatic_factors_in_Atlantic_salmon/4837938/1 unknown figshare https://dx.doi.org/10.1186/s12864-020-6513-4 https://dx.doi.org/10.6084/m9.figshare.c.4837938 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Cell Biology Genetics FOS Biological sciences Molecular Biology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry Computational Biology Collection article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.c.4837938.v1 https://doi.org/10.1186/s12864-020-6513-4 https://doi.org/10.6084/m9.figshare.c.4837938 2021-11-05T12:55:41Z Abstract Background Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell- and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells. Article in Journal/Newspaper Atlantic salmon DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
unknown |
topic |
Cell Biology Genetics FOS Biological sciences Molecular Biology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry Computational Biology |
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Cell Biology Genetics FOS Biological sciences Molecular Biology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry Computational Biology Kleppe, Lene Edvardsen, Rolf Furmanek, Tomasz Andersson, Eva Skaftnesmo, Kai Segafredo, Frida Thyri Wargelius, Anna Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
topic_facet |
Cell Biology Genetics FOS Biological sciences Molecular Biology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry Computational Biology |
description |
Abstract Background Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell- and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells. |
format |
Article in Journal/Newspaper |
author |
Kleppe, Lene Edvardsen, Rolf Furmanek, Tomasz Andersson, Eva Skaftnesmo, Kai Segafredo, Frida Thyri Wargelius, Anna |
author_facet |
Kleppe, Lene Edvardsen, Rolf Furmanek, Tomasz Andersson, Eva Skaftnesmo, Kai Segafredo, Frida Thyri Wargelius, Anna |
author_sort |
Kleppe, Lene |
title |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_short |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_full |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_fullStr |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_full_unstemmed |
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon |
title_sort |
transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in atlantic salmon |
publisher |
figshare |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4837938.v1 https://springernature.figshare.com/collections/Transcriptomic_analysis_of_dead_end_knockout_testis_reveals_germ_cell_and_gonadal_somatic_factors_in_Atlantic_salmon/4837938/1 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://dx.doi.org/10.1186/s12864-020-6513-4 https://dx.doi.org/10.6084/m9.figshare.c.4837938 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4837938.v1 https://doi.org/10.1186/s12864-020-6513-4 https://doi.org/10.6084/m9.figshare.c.4837938 |
_version_ |
1766362866438373376 |