Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome
Abstract Background As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotyp...
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ftdatacite:10.6084/m9.figshare.c.4823850 2023-05-15T15:58:50+02:00 Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Pierre-Louis Stenger Dégremont, Lionel Montagnani, Caroline Jean-Michel Escoubas Haffner, Philippe Jean-François Allienne Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jérémie Gueguen, Yannick Mitta, Guillaume 2020 https://dx.doi.org/10.6084/m9.figshare.c.4823850 https://springernature.figshare.com/collections/Differential_basal_expression_of_immune_genes_confers_Crassostrea_gigas_resistance_to_Pacific_oyster_mortality_syndrome/4823850 unknown figshare https://dx.doi.org/10.1186/s12864-020-6471-x CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Genetics FOS Biological sciences Evolutionary Biology Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer 111714 Mental Health FOS Health sciences 110309 Infectious Diseases Collection article 2020 ftdatacite https://doi.org/10.6084/m9.figshare.c.4823850 https://doi.org/10.1186/s12864-020-6471-x 2021-11-05T12:55:41Z Abstract Background As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. Conclusions We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function. Article in Journal/Newspaper Crassostrea gigas Pacific oyster DataCite Metadata Store (German National Library of Science and Technology) Pacific |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Genetics FOS Biological sciences Evolutionary Biology Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer 111714 Mental Health FOS Health sciences 110309 Infectious Diseases |
spellingShingle |
Genetics FOS Biological sciences Evolutionary Biology Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer 111714 Mental Health FOS Health sciences 110309 Infectious Diseases Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Pierre-Louis Stenger Dégremont, Lionel Montagnani, Caroline Jean-Michel Escoubas Haffner, Philippe Jean-François Allienne Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jérémie Gueguen, Yannick Mitta, Guillaume Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome |
topic_facet |
Genetics FOS Biological sciences Evolutionary Biology Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer 111714 Mental Health FOS Health sciences 110309 Infectious Diseases |
description |
Abstract Background As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. Conclusions We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function. |
format |
Article in Journal/Newspaper |
author |
Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Pierre-Louis Stenger Dégremont, Lionel Montagnani, Caroline Jean-Michel Escoubas Haffner, Philippe Jean-François Allienne Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jérémie Gueguen, Yannick Mitta, Guillaume |
author_facet |
Lorgeril, Julien Petton, Bruno Lucasson, Aude Perez, Valérie Pierre-Louis Stenger Dégremont, Lionel Montagnani, Caroline Jean-Michel Escoubas Haffner, Philippe Jean-François Allienne Leroy, Marc Lagarde, Franck Vidal-Dupiol, Jérémie Gueguen, Yannick Mitta, Guillaume |
author_sort |
Lorgeril, Julien |
title |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome |
title_short |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome |
title_full |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome |
title_fullStr |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome |
title_full_unstemmed |
Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome |
title_sort |
differential basal expression of immune genes confers crassostrea gigas resistance to pacific oyster mortality syndrome |
publisher |
figshare |
publishDate |
2020 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4823850 https://springernature.figshare.com/collections/Differential_basal_expression_of_immune_genes_confers_Crassostrea_gigas_resistance_to_Pacific_oyster_mortality_syndrome/4823850 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://dx.doi.org/10.1186/s12864-020-6471-x |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4823850 https://doi.org/10.1186/s12864-020-6471-x |
_version_ |
1766394595477815296 |