Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima?
Abstract Background Kelps (Laminariales, Phaeophyceae) are brown macroalgae of utmost ecological, and increasingly economic, importance on temperate to polar rocky shores. Omics approaches in brown algae are still scarce and knowledge of their acclimation mechanisms to the changing conditions experi...
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ftdatacite:10.6084/m9.figshare.c.4762556 2023-05-15T15:18:48+02:00 Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima? Monteiro, Cátia Machado Huiru Li Bischof, Kai Bartsch, Inka Valentin, Klaus Corre, Erwan Collén, Jonas Harms, Lars Glöckner, Gernot Heinrich, Sandra 2019 https://dx.doi.org/10.6084/m9.figshare.c.4762556 https://springernature.figshare.com/collections/Is_geographical_variation_driving_the_transcriptomic_responses_to_multiple_stressors_in_the_kelp_Saccharina_latissima_/4762556 unknown figshare https://dx.doi.org/10.1186/s12870-019-2124-0 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Genetics FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Collection article 2019 ftdatacite https://doi.org/10.6084/m9.figshare.c.4762556 https://doi.org/10.1186/s12870-019-2124-0 2021-11-05T12:55:41Z Abstract Background Kelps (Laminariales, Phaeophyceae) are brown macroalgae of utmost ecological, and increasingly economic, importance on temperate to polar rocky shores. Omics approaches in brown algae are still scarce and knowledge of their acclimation mechanisms to the changing conditions experienced in coastal environments can benefit from the application of RNA-sequencing. Despite evidence of ecotypic differentiation, transcriptomic responses from distinct geographical locations have, to our knowledge, never been studied in the sugar kelp Saccharina latissima so far. Results In this study we investigated gene expression responses using RNA-sequencing of S. latissima from environments with contrasting temperature and salinity conditions – Roscoff, in temperate eastern Atlantic, and Spitsbergen in the Arctic. Juvenile sporophytes derived from uniparental stock cultures from both locations were pre-cultivated at 8 °C and SA 30. Sporophytes acclimated to 0 °C, 8 °C and 15 °C were exposed to a low salinity treatment (SA 20) for 24 h. Hyposalinity had a greater impact at the transcriptomic level than the temperature alone, and its effects were modulated by temperature. Namely, photosynthesis and pigment synthesis were extensively repressed by low salinity at low temperatures. Although some responses were shared among sporophytes from the different sites, marked differences were revealed by principal component analysis, differential expression and GO enrichment. The interaction between low temperature and low salinity drove the largest changes in gene expression in sporophytes from Roscoff while specimens from Spitsbergen required more metabolic adjustment at higher temperatures. Moreover, genes related to cell wall adjustment were differentially expressed between Spitsbergen and Roscoff control samples. Conclusions Our study reveals interactive effects of temperature and salinity on transcriptomic profiles in S. latissima. Moreover, our data suggest that under identical culture conditions sporophytes from different locations diverge in their transcriptomic responses. This is probably connected to variations in temperature and salinity in their respective environment of origin. The current transcriptomic results support the plastic response pattern in sugar kelp which is a species with several reported ecotypes. Our data provide the baseline for a better understanding of the underlying processes of physiological plasticity and may help in the future to identify strains adapted to specific environments and its genetic control. Article in Journal/Newspaper Arctic Spitsbergen DataCite Metadata Store (German National Library of Science and Technology) Arctic |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Genetics FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
spellingShingle |
Genetics FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Monteiro, Cátia Machado Huiru Li Bischof, Kai Bartsch, Inka Valentin, Klaus Corre, Erwan Collén, Jonas Harms, Lars Glöckner, Gernot Heinrich, Sandra Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima? |
topic_facet |
Genetics FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
description |
Abstract Background Kelps (Laminariales, Phaeophyceae) are brown macroalgae of utmost ecological, and increasingly economic, importance on temperate to polar rocky shores. Omics approaches in brown algae are still scarce and knowledge of their acclimation mechanisms to the changing conditions experienced in coastal environments can benefit from the application of RNA-sequencing. Despite evidence of ecotypic differentiation, transcriptomic responses from distinct geographical locations have, to our knowledge, never been studied in the sugar kelp Saccharina latissima so far. Results In this study we investigated gene expression responses using RNA-sequencing of S. latissima from environments with contrasting temperature and salinity conditions – Roscoff, in temperate eastern Atlantic, and Spitsbergen in the Arctic. Juvenile sporophytes derived from uniparental stock cultures from both locations were pre-cultivated at 8 °C and SA 30. Sporophytes acclimated to 0 °C, 8 °C and 15 °C were exposed to a low salinity treatment (SA 20) for 24 h. Hyposalinity had a greater impact at the transcriptomic level than the temperature alone, and its effects were modulated by temperature. Namely, photosynthesis and pigment synthesis were extensively repressed by low salinity at low temperatures. Although some responses were shared among sporophytes from the different sites, marked differences were revealed by principal component analysis, differential expression and GO enrichment. The interaction between low temperature and low salinity drove the largest changes in gene expression in sporophytes from Roscoff while specimens from Spitsbergen required more metabolic adjustment at higher temperatures. Moreover, genes related to cell wall adjustment were differentially expressed between Spitsbergen and Roscoff control samples. Conclusions Our study reveals interactive effects of temperature and salinity on transcriptomic profiles in S. latissima. Moreover, our data suggest that under identical culture conditions sporophytes from different locations diverge in their transcriptomic responses. This is probably connected to variations in temperature and salinity in their respective environment of origin. The current transcriptomic results support the plastic response pattern in sugar kelp which is a species with several reported ecotypes. Our data provide the baseline for a better understanding of the underlying processes of physiological plasticity and may help in the future to identify strains adapted to specific environments and its genetic control. |
format |
Article in Journal/Newspaper |
author |
Monteiro, Cátia Machado Huiru Li Bischof, Kai Bartsch, Inka Valentin, Klaus Corre, Erwan Collén, Jonas Harms, Lars Glöckner, Gernot Heinrich, Sandra |
author_facet |
Monteiro, Cátia Machado Huiru Li Bischof, Kai Bartsch, Inka Valentin, Klaus Corre, Erwan Collén, Jonas Harms, Lars Glöckner, Gernot Heinrich, Sandra |
author_sort |
Monteiro, Cátia Machado |
title |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima? |
title_short |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima? |
title_full |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima? |
title_fullStr |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima? |
title_full_unstemmed |
Is geographical variation driving the transcriptomic responses to multiple stressors in the kelp Saccharina latissima? |
title_sort |
is geographical variation driving the transcriptomic responses to multiple stressors in the kelp saccharina latissima? |
publisher |
figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4762556 https://springernature.figshare.com/collections/Is_geographical_variation_driving_the_transcriptomic_responses_to_multiple_stressors_in_the_kelp_Saccharina_latissima_/4762556 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_relation |
https://dx.doi.org/10.1186/s12870-019-2124-0 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4762556 https://doi.org/10.1186/s12870-019-2124-0 |
_version_ |
1766348982232023040 |