The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status
Abstract Background When puberty starts before males reach harvest size, animal welfare and sustainability issues occur in Atlantic salmon (Salmo salar) aquaculture. Hallmarks of male puberty are an increased proliferation activity in the testis and elevated androgen production. Examining transcript...
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ftdatacite:10.6084/m9.figshare.c.4538153 2023-05-15T15:32:21+02:00 The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status Crespo, Diego Bogerd, Jan Sambroni, Elisabeth LeGac, Florence Andersson, Eva Edvardsen, Rolf Bergman, Elisabeth Björnsson, Björn Taranger, Geir Schulz, Rüdiger 2019 https://dx.doi.org/10.6084/m9.figshare.c.4538153 https://springernature.figshare.com/collections/The_initiation_of_puberty_in_Atlantic_salmon_brings_about_large_changes_in_testicular_gene_expression_that_are_modulated_by_the_energy_status/4538153 unknown Figshare https://dx.doi.org/10.1186/s12864-019-5869-9 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Medicine Cell Biology Genetics FOS Biological sciences Molecular Biology Physiology Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Developmental Biology Inorganic Chemistry FOS Chemical sciences 111714 Mental Health FOS Health sciences Computational Biology Collection article 2019 ftdatacite https://doi.org/10.6084/m9.figshare.c.4538153 https://doi.org/10.1186/s12864-019-5869-9 2021-11-05T12:55:41Z Abstract Background When puberty starts before males reach harvest size, animal welfare and sustainability issues occur in Atlantic salmon (Salmo salar) aquaculture. Hallmarks of male puberty are an increased proliferation activity in the testis and elevated androgen production. Examining transcriptional changes in salmon testis during the transition from immature to maturing testes may help understanding the regulation of puberty, potentially leading to procedures to modulate its start. Since differences in body weight influence, via unknown mechanisms, the chances for entering puberty, we used two feed rations to create body weight differences. Results Maturing testes were characterized by an elevated proliferation activity of Sertoli cells and of single undifferentiated spermatogonia. Pituitary gene expression data suggest increased Gnrh receptor and gonadotropin gene expression, potentially responsible for the elevated circulating androgen levels in maturing fish. Transcriptional changes in maturing testes included a broad variety of signaling systems (e.g. Tgfβ, Wnt, insulin/Igf, nuclear receptors), but also, activation of metabolic pathways such as anaerobic metabolism and protection against ROS. Feed restriction lowered the incidence of puberty. In males maturing despite feed restriction, plasma androgen levels were higher than in maturing fish receiving the full ration. A group of 449 genes that were up-regulated in maturing fully fed fish, was up-regulated more prominently in testis from fish maturing under caloric restriction. Moreover, 421 genes were specifically up-regulated in testes from fish maturing under caloric restriction, including carbon metabolism genes, a pathway relevant for nucleotide biosynthesis and for placing epigenetic marks. Conclusions Undifferentiated spermatogonia and Sertoli cell populations increased at the beginning of puberty, which was associated with the up-regulation of metabolic pathways (e.g. anaerobic and ROS pathways) known from other stem cell systems. The higher androgen levels in males maturing under caloric restriction may be responsible for the stronger up-regulation of a common set of (449) maturation-associated genes, and the specific up-regulation of another set of (421) genes. The latter opened regulatory and/or metabolic options for initiating puberty despite feed restriction. As a means to reduce the incidence of male puberty in salmon, however, caloric restriction seems unsuitable. Article in Journal/Newspaper Atlantic salmon Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Medicine Cell Biology Genetics FOS Biological sciences Molecular Biology Physiology Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Developmental Biology Inorganic Chemistry FOS Chemical sciences 111714 Mental Health FOS Health sciences Computational Biology |
spellingShingle |
Biochemistry Medicine Cell Biology Genetics FOS Biological sciences Molecular Biology Physiology Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Developmental Biology Inorganic Chemistry FOS Chemical sciences 111714 Mental Health FOS Health sciences Computational Biology Crespo, Diego Bogerd, Jan Sambroni, Elisabeth LeGac, Florence Andersson, Eva Edvardsen, Rolf Bergman, Elisabeth Björnsson, Björn Taranger, Geir Schulz, Rüdiger The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status |
topic_facet |
Biochemistry Medicine Cell Biology Genetics FOS Biological sciences Molecular Biology Physiology Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Developmental Biology Inorganic Chemistry FOS Chemical sciences 111714 Mental Health FOS Health sciences Computational Biology |
description |
Abstract Background When puberty starts before males reach harvest size, animal welfare and sustainability issues occur in Atlantic salmon (Salmo salar) aquaculture. Hallmarks of male puberty are an increased proliferation activity in the testis and elevated androgen production. Examining transcriptional changes in salmon testis during the transition from immature to maturing testes may help understanding the regulation of puberty, potentially leading to procedures to modulate its start. Since differences in body weight influence, via unknown mechanisms, the chances for entering puberty, we used two feed rations to create body weight differences. Results Maturing testes were characterized by an elevated proliferation activity of Sertoli cells and of single undifferentiated spermatogonia. Pituitary gene expression data suggest increased Gnrh receptor and gonadotropin gene expression, potentially responsible for the elevated circulating androgen levels in maturing fish. Transcriptional changes in maturing testes included a broad variety of signaling systems (e.g. Tgfβ, Wnt, insulin/Igf, nuclear receptors), but also, activation of metabolic pathways such as anaerobic metabolism and protection against ROS. Feed restriction lowered the incidence of puberty. In males maturing despite feed restriction, plasma androgen levels were higher than in maturing fish receiving the full ration. A group of 449 genes that were up-regulated in maturing fully fed fish, was up-regulated more prominently in testis from fish maturing under caloric restriction. Moreover, 421 genes were specifically up-regulated in testes from fish maturing under caloric restriction, including carbon metabolism genes, a pathway relevant for nucleotide biosynthesis and for placing epigenetic marks. Conclusions Undifferentiated spermatogonia and Sertoli cell populations increased at the beginning of puberty, which was associated with the up-regulation of metabolic pathways (e.g. anaerobic and ROS pathways) known from other stem cell systems. The higher androgen levels in males maturing under caloric restriction may be responsible for the stronger up-regulation of a common set of (449) maturation-associated genes, and the specific up-regulation of another set of (421) genes. The latter opened regulatory and/or metabolic options for initiating puberty despite feed restriction. As a means to reduce the incidence of male puberty in salmon, however, caloric restriction seems unsuitable. |
format |
Article in Journal/Newspaper |
author |
Crespo, Diego Bogerd, Jan Sambroni, Elisabeth LeGac, Florence Andersson, Eva Edvardsen, Rolf Bergman, Elisabeth Björnsson, Björn Taranger, Geir Schulz, Rüdiger |
author_facet |
Crespo, Diego Bogerd, Jan Sambroni, Elisabeth LeGac, Florence Andersson, Eva Edvardsen, Rolf Bergman, Elisabeth Björnsson, Björn Taranger, Geir Schulz, Rüdiger |
author_sort |
Crespo, Diego |
title |
The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status |
title_short |
The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status |
title_full |
The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status |
title_fullStr |
The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status |
title_full_unstemmed |
The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status |
title_sort |
initiation of puberty in atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status |
publisher |
Figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4538153 https://springernature.figshare.com/collections/The_initiation_of_puberty_in_Atlantic_salmon_brings_about_large_changes_in_testicular_gene_expression_that_are_modulated_by_the_energy_status/4538153 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://dx.doi.org/10.1186/s12864-019-5869-9 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4538153 https://doi.org/10.1186/s12864-019-5869-9 |
_version_ |
1766362865267113984 |