Supplementary material from "Functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish"
A successful spawning migration in salmon depends on their athletic ability, and thus, on efficient cardiovascular oxygen (O 2 ) transport. Most teleost fishes have highly pH-sensitive haemoglobins (Hb) that can release large amounts of O 2 when the blood is acidified at the tissues. We hypothesized...
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ftdatacite:10.6084/m9.figshare.c.4508273.v1 2023-05-15T15:32:33+02:00 Supplementary material from "Functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish" T. S. Harter F. S. Zanuzzo C. T. Supuran A. K. Gamperl C. J. Brauner 2019 https://dx.doi.org/10.6084/m9.figshare.c.4508273.v1 https://rs.figshare.com/collections/Supplementary_material_from_Functional_support_for_a_novel_mechanism_that_enhances_tissue_oxygen_extraction_in_a_teleost_fish_/4508273/1 unknown Figshare https://dx.doi.org/10.1098/rspb.2019.0339 https://dx.doi.org/10.6084/m9.figshare.c.4508273 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Physiology FOS Biological sciences Collection article 2019 ftdatacite https://doi.org/10.6084/m9.figshare.c.4508273.v1 https://doi.org/10.1098/rspb.2019.0339 https://doi.org/10.6084/m9.figshare.c.4508273 2021-11-05T12:55:41Z A successful spawning migration in salmon depends on their athletic ability, and thus, on efficient cardiovascular oxygen (O 2 ) transport. Most teleost fishes have highly pH-sensitive haemoglobins (Hb) that can release large amounts of O 2 when the blood is acidified at the tissues. We hypothesized that plasma-accessible carbonic anhydrase (paCA; the enzyme that catalyses proton production from CO 2 ) is required to acidify the blood at the tissues and promote tissue O 2 extraction. Previous studies have reported an elevated tissue O 2 extraction in hypoxia-acclimated teleosts that may also be facilitated by paCA. Thus, to create experimental contrasts in tissue O 2 extraction, Atlantic salmon were acclimated to normoxia or hypoxia (40% air saturation, greater than six weeks), and the role of paCA in enhancing tissue O 2 extraction was tested by inhibiting paCA at rest and during submaximal exercise. Our results show that: (i) in both acclimation groups, the inhibition of paCA increased cardiac output by one-third, indicating a role of paCA in promoting tissue O 2 extraction during exercise, recovery and at rest; (ii) the recruitment of paCA was plastic and increased following hypoxic acclimation; and (iii) maximal exercise performance in salmon, and thus a successful spawning migration, may not be possible without paCA. Article in Journal/Newspaper Atlantic salmon DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Physiology FOS Biological sciences |
spellingShingle |
Biochemistry Physiology FOS Biological sciences T. S. Harter F. S. Zanuzzo C. T. Supuran A. K. Gamperl C. J. Brauner Supplementary material from "Functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish" |
topic_facet |
Biochemistry Physiology FOS Biological sciences |
description |
A successful spawning migration in salmon depends on their athletic ability, and thus, on efficient cardiovascular oxygen (O 2 ) transport. Most teleost fishes have highly pH-sensitive haemoglobins (Hb) that can release large amounts of O 2 when the blood is acidified at the tissues. We hypothesized that plasma-accessible carbonic anhydrase (paCA; the enzyme that catalyses proton production from CO 2 ) is required to acidify the blood at the tissues and promote tissue O 2 extraction. Previous studies have reported an elevated tissue O 2 extraction in hypoxia-acclimated teleosts that may also be facilitated by paCA. Thus, to create experimental contrasts in tissue O 2 extraction, Atlantic salmon were acclimated to normoxia or hypoxia (40% air saturation, greater than six weeks), and the role of paCA in enhancing tissue O 2 extraction was tested by inhibiting paCA at rest and during submaximal exercise. Our results show that: (i) in both acclimation groups, the inhibition of paCA increased cardiac output by one-third, indicating a role of paCA in promoting tissue O 2 extraction during exercise, recovery and at rest; (ii) the recruitment of paCA was plastic and increased following hypoxic acclimation; and (iii) maximal exercise performance in salmon, and thus a successful spawning migration, may not be possible without paCA. |
format |
Article in Journal/Newspaper |
author |
T. S. Harter F. S. Zanuzzo C. T. Supuran A. K. Gamperl C. J. Brauner |
author_facet |
T. S. Harter F. S. Zanuzzo C. T. Supuran A. K. Gamperl C. J. Brauner |
author_sort |
T. S. Harter |
title |
Supplementary material from "Functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish" |
title_short |
Supplementary material from "Functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish" |
title_full |
Supplementary material from "Functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish" |
title_fullStr |
Supplementary material from "Functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish" |
title_full_unstemmed |
Supplementary material from "Functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish" |
title_sort |
supplementary material from "functional support for a novel mechanism that enhances tissue oxygen extraction in a teleost fish" |
publisher |
Figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4508273.v1 https://rs.figshare.com/collections/Supplementary_material_from_Functional_support_for_a_novel_mechanism_that_enhances_tissue_oxygen_extraction_in_a_teleost_fish_/4508273/1 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://dx.doi.org/10.1098/rspb.2019.0339 https://dx.doi.org/10.6084/m9.figshare.c.4508273 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4508273.v1 https://doi.org/10.1098/rspb.2019.0339 https://doi.org/10.6084/m9.figshare.c.4508273 |
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