Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China
Abstract Background Papillomaviruses (PVs) and polyomaviruses (PyVs) infect diverse vertebrates including human and cause a broad spectrum of outcomes from asymptomatic infection to severe disease. There has been no PV and only one PyV detected in tree shrews, though the genomic properties of tree s...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Figshare
2019
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.4438595 https://springernature.figshare.com/collections/Detection_and_genome_characterization_of_two_novel_papillomaviruses_and_a_novel_polyomavirus_in_tree_shrew_Tupaia_belangeri_chinensis_in_China/4438595 |
id |
ftdatacite:10.6084/m9.figshare.c.4438595 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6084/m9.figshare.c.4438595 2023-05-15T18:42:26+02:00 Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China Liu, Ping Qiu, Ye Xing, Cheng Zhou, Ji-Hua Yang, Wei-Hong Wang, Qiong Li, Jin-Yan Han, Xi Zhang, Yun-Zhi Xing-Yi Ge 2019 https://dx.doi.org/10.6084/m9.figshare.c.4438595 https://springernature.figshare.com/collections/Detection_and_genome_characterization_of_two_novel_papillomaviruses_and_a_novel_polyomavirus_in_tree_shrew_Tupaia_belangeri_chinensis_in_China/4438595 unknown Figshare https://dx.doi.org/10.1186/s12985-019-1141-9 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Genetics FOS Biological sciences Biotechnology Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Cancer 110309 Infectious Diseases FOS Health sciences Plant Biology 60506 Virology Collection article 2019 ftdatacite https://doi.org/10.6084/m9.figshare.c.4438595 https://doi.org/10.1186/s12985-019-1141-9 2021-11-05T12:55:41Z Abstract Background Papillomaviruses (PVs) and polyomaviruses (PyVs) infect diverse vertebrates including human and cause a broad spectrum of outcomes from asymptomatic infection to severe disease. There has been no PV and only one PyV detected in tree shrews, though the genomic properties of tree shrews are highly similar to those of the primates. Methods Swab and organ samples of tree shrews collected in the Yunnan Province of China, were tested by viral metagenomic analysis and random PCR to detect the presence of PVs and PyVs. By PCR amplification using specific primers, cloning, sequencing and assembling, genomes of two PVs and one PyV were identified in the samples. Results Two novel PVs and a novel PyV, named tree shrew papillomavirus 1 and 2 (TbelPV1 and TbelPV2) and polyomavirus 1 (TbelPyV1) were characterized in the Chinese tree shrew (Tupaia belangeri chinensis). The genomes of TbelPV1, TbelPV2, and TbelPyV1 are 7410â bp, 7526â bp, and 4982â bp in size, respectively. The TbelPV1 genome contains 7 putative open-reading frames (ORFs) coding for viral proteins E1, E2, E4, E6, E7, L1, and L2; the TbelPV2 genome contains 6 ORFs coding for viral proteins E1, E2, E6, E7, L1, and L2; and the TbelPyV1 genome codes for the typical small and large T antigens of PyV, as well as the VP1, VP2, and VP3 capsid proteins. Genomic comparison and phylogenetic analysis indicated that TbelPV1 and TbelPV2 represented 2 novel PV genera of Papillomaviridae, and TbelPyV1 represented a new species of genus Alphapolyomavirus. Our epidemiologic study indicated that TbelPV1 and TbelPV2 were both detected in oral swabs, while TbelPyV1 was detected in oral swabs and spleens. Conclusion Two novel PVs (TbelPV1 and TbelPV2) and a novel PyV (TbelPyV) were discovered in tree shrews and their genomes were characterized. TbelPV1, TbelPV2, and TbelPyV1 have the highest similarity to Human papillomavirus type 63, Ursus maritimus papillomavirus 1, and Human polyomavirus 9, respectively. TbelPV1 and TbelPV2 only showed oral tropism, while TbelPyV1 showed oral and spleen tropism. Article in Journal/Newspaper Ursus maritimus DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Genetics FOS Biological sciences Biotechnology Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Cancer 110309 Infectious Diseases FOS Health sciences Plant Biology 60506 Virology |
spellingShingle |
Genetics FOS Biological sciences Biotechnology Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Cancer 110309 Infectious Diseases FOS Health sciences Plant Biology 60506 Virology Liu, Ping Qiu, Ye Xing, Cheng Zhou, Ji-Hua Yang, Wei-Hong Wang, Qiong Li, Jin-Yan Han, Xi Zhang, Yun-Zhi Xing-Yi Ge Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China |
topic_facet |
Genetics FOS Biological sciences Biotechnology Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Cancer 110309 Infectious Diseases FOS Health sciences Plant Biology 60506 Virology |
description |
Abstract Background Papillomaviruses (PVs) and polyomaviruses (PyVs) infect diverse vertebrates including human and cause a broad spectrum of outcomes from asymptomatic infection to severe disease. There has been no PV and only one PyV detected in tree shrews, though the genomic properties of tree shrews are highly similar to those of the primates. Methods Swab and organ samples of tree shrews collected in the Yunnan Province of China, were tested by viral metagenomic analysis and random PCR to detect the presence of PVs and PyVs. By PCR amplification using specific primers, cloning, sequencing and assembling, genomes of two PVs and one PyV were identified in the samples. Results Two novel PVs and a novel PyV, named tree shrew papillomavirus 1 and 2 (TbelPV1 and TbelPV2) and polyomavirus 1 (TbelPyV1) were characterized in the Chinese tree shrew (Tupaia belangeri chinensis). The genomes of TbelPV1, TbelPV2, and TbelPyV1 are 7410â bp, 7526â bp, and 4982â bp in size, respectively. The TbelPV1 genome contains 7 putative open-reading frames (ORFs) coding for viral proteins E1, E2, E4, E6, E7, L1, and L2; the TbelPV2 genome contains 6 ORFs coding for viral proteins E1, E2, E6, E7, L1, and L2; and the TbelPyV1 genome codes for the typical small and large T antigens of PyV, as well as the VP1, VP2, and VP3 capsid proteins. Genomic comparison and phylogenetic analysis indicated that TbelPV1 and TbelPV2 represented 2 novel PV genera of Papillomaviridae, and TbelPyV1 represented a new species of genus Alphapolyomavirus. Our epidemiologic study indicated that TbelPV1 and TbelPV2 were both detected in oral swabs, while TbelPyV1 was detected in oral swabs and spleens. Conclusion Two novel PVs (TbelPV1 and TbelPV2) and a novel PyV (TbelPyV) were discovered in tree shrews and their genomes were characterized. TbelPV1, TbelPV2, and TbelPyV1 have the highest similarity to Human papillomavirus type 63, Ursus maritimus papillomavirus 1, and Human polyomavirus 9, respectively. TbelPV1 and TbelPV2 only showed oral tropism, while TbelPyV1 showed oral and spleen tropism. |
format |
Article in Journal/Newspaper |
author |
Liu, Ping Qiu, Ye Xing, Cheng Zhou, Ji-Hua Yang, Wei-Hong Wang, Qiong Li, Jin-Yan Han, Xi Zhang, Yun-Zhi Xing-Yi Ge |
author_facet |
Liu, Ping Qiu, Ye Xing, Cheng Zhou, Ji-Hua Yang, Wei-Hong Wang, Qiong Li, Jin-Yan Han, Xi Zhang, Yun-Zhi Xing-Yi Ge |
author_sort |
Liu, Ping |
title |
Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China |
title_short |
Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China |
title_full |
Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China |
title_fullStr |
Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China |
title_full_unstemmed |
Detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (Tupaia belangeri chinensis) in China |
title_sort |
detection and genome characterization of two novel papillomaviruses and a novel polyomavirus in tree shrew (tupaia belangeri chinensis) in china |
publisher |
Figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4438595 https://springernature.figshare.com/collections/Detection_and_genome_characterization_of_two_novel_papillomaviruses_and_a_novel_polyomavirus_in_tree_shrew_Tupaia_belangeri_chinensis_in_China/4438595 |
genre |
Ursus maritimus |
genre_facet |
Ursus maritimus |
op_relation |
https://dx.doi.org/10.1186/s12985-019-1141-9 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4438595 https://doi.org/10.1186/s12985-019-1141-9 |
_version_ |
1766232098427895808 |