Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss)
Abstract Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in different salmonid species. PRV-1 causes heart and skeletal muscle inflammation (HSMI) in Atlantic...
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ftdatacite:10.6084/m9.figshare.c.4406000 2023-05-15T15:31:44+02:00 Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) NiccolĂł Vendramin Dhamotharan Kannimuthu Olsen, Anne Argelia Cuenca Teige, Lena ĂYstein Wessel Iburg, Tine Dahle, Maria Rimstad, Espen Olesen, Niels 2019 https://dx.doi.org/10.6084/m9.figshare.c.4406000 https://springernature.figshare.com/collections/Piscine_orthoreovirus_subtype_3_PRV-3_causes_heart_inflammation_in_rainbow_trout_Oncorhynchus_mykiss_/4406000 unknown Figshare https://dx.doi.org/10.1186/s13567-019-0632-4 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Medicine 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified 60506 Virology Collection article 2019 ftdatacite https://doi.org/10.6084/m9.figshare.c.4406000 https://doi.org/10.1186/s13567-019-0632-4 2021-11-05T12:55:41Z Abstract Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in different salmonid species. PRV-1 causes heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar) and is associated with jaundice syndrome in farmed chinook salmon (Oncorhynchus tshawytscha). PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon in Japan. PRV-3 has recently been associated with a disease in rainbow trout (Oncorhynchus mykiss) characterized by anaemia, heart and red muscle pathology; to jaundice syndrome in coho salmon (Oncorhynchus kisutch). In this study, we conducted a 10-week long experimental infection trial in rainbow trout with purified PRV-3 particles to assess the causal relationship between the virus and development of heart inflammation. The monitoring the PRV-3 load in heart and spleen by RT-qPCR shows a progressive increase of viral RNA to a peak, followed by clearance without a measurable change in haematocrit. The development of characteristic cardiac histopathological findings occurred in the late phase of the trial and was associated with increased expression of CD8+, indicating cytotoxic T cell proliferation. The findings indicate that, under these experimental conditions, PRV-3 infection in rainbow trout act similarly to PRV-1 infection in Atlantic salmon with regards to immunological responses and development of heart pathology, but not in the ability to establish a persistent infection. Article in Journal/Newspaper Atlantic salmon Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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Biochemistry Medicine 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified 60506 Virology |
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Biochemistry Medicine 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified 60506 Virology NiccolĂł Vendramin Dhamotharan Kannimuthu Olsen, Anne Argelia Cuenca Teige, Lena ĂYstein Wessel Iburg, Tine Dahle, Maria Rimstad, Espen Olesen, Niels Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
topic_facet |
Biochemistry Medicine 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified 60506 Virology |
description |
Abstract Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in different salmonid species. PRV-1 causes heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar) and is associated with jaundice syndrome in farmed chinook salmon (Oncorhynchus tshawytscha). PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon in Japan. PRV-3 has recently been associated with a disease in rainbow trout (Oncorhynchus mykiss) characterized by anaemia, heart and red muscle pathology; to jaundice syndrome in coho salmon (Oncorhynchus kisutch). In this study, we conducted a 10-week long experimental infection trial in rainbow trout with purified PRV-3 particles to assess the causal relationship between the virus and development of heart inflammation. The monitoring the PRV-3 load in heart and spleen by RT-qPCR shows a progressive increase of viral RNA to a peak, followed by clearance without a measurable change in haematocrit. The development of characteristic cardiac histopathological findings occurred in the late phase of the trial and was associated with increased expression of CD8+, indicating cytotoxic T cell proliferation. The findings indicate that, under these experimental conditions, PRV-3 infection in rainbow trout act similarly to PRV-1 infection in Atlantic salmon with regards to immunological responses and development of heart pathology, but not in the ability to establish a persistent infection. |
format |
Article in Journal/Newspaper |
author |
NiccolĂł Vendramin Dhamotharan Kannimuthu Olsen, Anne Argelia Cuenca Teige, Lena ĂYstein Wessel Iburg, Tine Dahle, Maria Rimstad, Espen Olesen, Niels |
author_facet |
NiccolĂł Vendramin Dhamotharan Kannimuthu Olsen, Anne Argelia Cuenca Teige, Lena ĂYstein Wessel Iburg, Tine Dahle, Maria Rimstad, Espen Olesen, Niels |
author_sort |
NiccolĂł Vendramin |
title |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_short |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_full |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_fullStr |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_full_unstemmed |
Piscine orthoreovirus subtype 3 (PRV-3) causes heart inflammation in rainbow trout (Oncorhynchus mykiss) |
title_sort |
piscine orthoreovirus subtype 3 (prv-3) causes heart inflammation in rainbow trout (oncorhynchus mykiss) |
publisher |
Figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4406000 https://springernature.figshare.com/collections/Piscine_orthoreovirus_subtype_3_PRV-3_causes_heart_inflammation_in_rainbow_trout_Oncorhynchus_mykiss_/4406000 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://dx.doi.org/10.1186/s13567-019-0632-4 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4406000 https://doi.org/10.1186/s13567-019-0632-4 |
_version_ |
1766362253021413376 |