Supplementary material from "Towards population genomics in non-model species with large genomes; a case study of the marine zooplankton Calanus finmarchicus "
Advances in next-generation sequencing technologies and the development of genome-reduced representation protocols have opened the way to genome-wide population studies in non-model species. However, species with large genomes remain challenging, hampering the development of genomic resources for a...
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ftdatacite:10.6084/m9.figshare.c.4382240 2023-05-15T15:47:55+02:00 Supplementary material from "Towards population genomics in non-model species with large genomes; a case study of the marine zooplankton Calanus finmarchicus " Choquet, Marvin Smolina, Irina Anusha K. S. Dhanasiri Blanco-Bercial, Leocadio Kopp, Martina Jueterbock, Alexander Sundaram, Arvind Y. M. Galice Hoarau 2019 https://dx.doi.org/10.6084/m9.figshare.c.4382240 https://rs.figshare.com/collections/Supplementary_material_from_Towards_population_genomics_in_non-model_species_with_large_genomes_a_case_study_of_the_marine_zooplankton_i_Calanus_finmarchicus_i_/4382240 unknown Figshare https://dx.doi.org/10.1098/rsos.180608 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY 60102 Bioinformatics FOS Computer and information sciences 60408 Genomics FOS Biological sciences Collection article 2019 ftdatacite https://doi.org/10.6084/m9.figshare.c.4382240 https://doi.org/10.1098/rsos.180608 2021-11-05T12:55:41Z Advances in next-generation sequencing technologies and the development of genome-reduced representation protocols have opened the way to genome-wide population studies in non-model species. However, species with large genomes remain challenging, hampering the development of genomic resources for a number of taxa including marine arthropods. Here, we developed a genome-reduced representation method for the ecologically important marine copepod Calanus finmarchicus (haploid genome size of 6.34 Gbp). We optimized a capture enrichment-based protocol based on 2656 single-copy genes, yielding a total of 154 087 high-quality SNPs in C. finmarchicus including 62 372 in common among the three locations tested. The set of capture probes was also successfully applied to the congeneric C. glacialis . Preliminary analyses of these markers revealed similar levels of genetic diversity between the two Calanus species, while populations of C. glacialis showed stronger genetic structure compared to C. finmarchicus . Using this powerful set of markers, we did not detect any evidence of hybridization between C. finmarchicus and C. glacialis . Finally, we propose a shortened version of our protocol, offering a promising solution for population genomics studies in non-model species with large genomes. Article in Journal/Newspaper Calanus finmarchicus DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
60102 Bioinformatics FOS Computer and information sciences 60408 Genomics FOS Biological sciences |
spellingShingle |
60102 Bioinformatics FOS Computer and information sciences 60408 Genomics FOS Biological sciences Choquet, Marvin Smolina, Irina Anusha K. S. Dhanasiri Blanco-Bercial, Leocadio Kopp, Martina Jueterbock, Alexander Sundaram, Arvind Y. M. Galice Hoarau Supplementary material from "Towards population genomics in non-model species with large genomes; a case study of the marine zooplankton Calanus finmarchicus " |
topic_facet |
60102 Bioinformatics FOS Computer and information sciences 60408 Genomics FOS Biological sciences |
description |
Advances in next-generation sequencing technologies and the development of genome-reduced representation protocols have opened the way to genome-wide population studies in non-model species. However, species with large genomes remain challenging, hampering the development of genomic resources for a number of taxa including marine arthropods. Here, we developed a genome-reduced representation method for the ecologically important marine copepod Calanus finmarchicus (haploid genome size of 6.34 Gbp). We optimized a capture enrichment-based protocol based on 2656 single-copy genes, yielding a total of 154 087 high-quality SNPs in C. finmarchicus including 62 372 in common among the three locations tested. The set of capture probes was also successfully applied to the congeneric C. glacialis . Preliminary analyses of these markers revealed similar levels of genetic diversity between the two Calanus species, while populations of C. glacialis showed stronger genetic structure compared to C. finmarchicus . Using this powerful set of markers, we did not detect any evidence of hybridization between C. finmarchicus and C. glacialis . Finally, we propose a shortened version of our protocol, offering a promising solution for population genomics studies in non-model species with large genomes. |
format |
Article in Journal/Newspaper |
author |
Choquet, Marvin Smolina, Irina Anusha K. S. Dhanasiri Blanco-Bercial, Leocadio Kopp, Martina Jueterbock, Alexander Sundaram, Arvind Y. M. Galice Hoarau |
author_facet |
Choquet, Marvin Smolina, Irina Anusha K. S. Dhanasiri Blanco-Bercial, Leocadio Kopp, Martina Jueterbock, Alexander Sundaram, Arvind Y. M. Galice Hoarau |
author_sort |
Choquet, Marvin |
title |
Supplementary material from "Towards population genomics in non-model species with large genomes; a case study of the marine zooplankton Calanus finmarchicus " |
title_short |
Supplementary material from "Towards population genomics in non-model species with large genomes; a case study of the marine zooplankton Calanus finmarchicus " |
title_full |
Supplementary material from "Towards population genomics in non-model species with large genomes; a case study of the marine zooplankton Calanus finmarchicus " |
title_fullStr |
Supplementary material from "Towards population genomics in non-model species with large genomes; a case study of the marine zooplankton Calanus finmarchicus " |
title_full_unstemmed |
Supplementary material from "Towards population genomics in non-model species with large genomes; a case study of the marine zooplankton Calanus finmarchicus " |
title_sort |
supplementary material from "towards population genomics in non-model species with large genomes; a case study of the marine zooplankton calanus finmarchicus " |
publisher |
Figshare |
publishDate |
2019 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4382240 https://rs.figshare.com/collections/Supplementary_material_from_Towards_population_genomics_in_non-model_species_with_large_genomes_a_case_study_of_the_marine_zooplankton_i_Calanus_finmarchicus_i_/4382240 |
genre |
Calanus finmarchicus |
genre_facet |
Calanus finmarchicus |
op_relation |
https://dx.doi.org/10.1098/rsos.180608 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4382240 https://doi.org/10.1098/rsos.180608 |
_version_ |
1766382886789840896 |