Supplementary material from "Ocean pH fluctuations affect mussel larvae at key developmental transitions"
Coastal marine ecosystems experience dynamic fluctuations in seawater carbonate chemistry. The importance of this variation in the context of ocean acidification requires knowing what aspect of variability biological processes respond to. We conducted four experiments (ranging from 3 to 22 days) wit...
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ftdatacite:10.6084/m9.figshare.c.4325828.v1 2023-05-15T17:49:54+02:00 Supplementary material from "Ocean pH fluctuations affect mussel larvae at key developmental transitions" L. Kapsenberg A. Miglioli M. C. Bitter E. Tambutté R. Dumollard J.-P. Gattuso 2018 https://dx.doi.org/10.6084/m9.figshare.c.4325828.v1 https://figshare.com/collections/Supplementary_material_from_Ocean_pH_fluctuations_affect_mussel_larvae_at_key_developmental_transitions_/4325828/1 unknown Figshare https://dx.doi.org/10.1098/rspb.2018.2381 https://dx.doi.org/10.6084/m9.figshare.c.4325828 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Developmental Biology Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4325828.v1 https://doi.org/10.1098/rspb.2018.2381 https://doi.org/10.6084/m9.figshare.c.4325828 2021-11-05T12:55:41Z Coastal marine ecosystems experience dynamic fluctuations in seawater carbonate chemistry. The importance of this variation in the context of ocean acidification requires knowing what aspect of variability biological processes respond to. We conducted four experiments (ranging from 3 to 22 days) with different variability regimes (pH T 7.4–8.1) assessing the impact of diel fluctuations in carbonate chemistry on the early development of the mussel Mytilus galloprovincialis . Larval shell growth was consistently correlated to mean exposures, regardless of variability regimes, indicating that calcification responds instantaneously to seawater chemistry. Larval development was impacted by timing of exposure, revealing sensitivity of two developmental processes: development of the shell field, and transition from the first to the second larval shell. Fluorescent staining revealed developmental delay of the shell field at low pH, and abnormal development thereof was correlated to hinge defects in D-veligers. This study shows, for the first time, that ocean acidification affects larval soft-tissue development, independent from calcification. Multiple developmental processes additively underpin the teratogenic effect of ocean acidification on bivalve larvae. These results explain why trochophores are the most sensitive life-history stage in marine bivalves and suggest that short-term variability in carbonate chemistry can impact early larval development. Article in Journal/Newspaper Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
Developmental Biology |
spellingShingle |
Developmental Biology L. Kapsenberg A. Miglioli M. C. Bitter E. Tambutté R. Dumollard J.-P. Gattuso Supplementary material from "Ocean pH fluctuations affect mussel larvae at key developmental transitions" |
topic_facet |
Developmental Biology |
description |
Coastal marine ecosystems experience dynamic fluctuations in seawater carbonate chemistry. The importance of this variation in the context of ocean acidification requires knowing what aspect of variability biological processes respond to. We conducted four experiments (ranging from 3 to 22 days) with different variability regimes (pH T 7.4–8.1) assessing the impact of diel fluctuations in carbonate chemistry on the early development of the mussel Mytilus galloprovincialis . Larval shell growth was consistently correlated to mean exposures, regardless of variability regimes, indicating that calcification responds instantaneously to seawater chemistry. Larval development was impacted by timing of exposure, revealing sensitivity of two developmental processes: development of the shell field, and transition from the first to the second larval shell. Fluorescent staining revealed developmental delay of the shell field at low pH, and abnormal development thereof was correlated to hinge defects in D-veligers. This study shows, for the first time, that ocean acidification affects larval soft-tissue development, independent from calcification. Multiple developmental processes additively underpin the teratogenic effect of ocean acidification on bivalve larvae. These results explain why trochophores are the most sensitive life-history stage in marine bivalves and suggest that short-term variability in carbonate chemistry can impact early larval development. |
format |
Article in Journal/Newspaper |
author |
L. Kapsenberg A. Miglioli M. C. Bitter E. Tambutté R. Dumollard J.-P. Gattuso |
author_facet |
L. Kapsenberg A. Miglioli M. C. Bitter E. Tambutté R. Dumollard J.-P. Gattuso |
author_sort |
L. Kapsenberg |
title |
Supplementary material from "Ocean pH fluctuations affect mussel larvae at key developmental transitions" |
title_short |
Supplementary material from "Ocean pH fluctuations affect mussel larvae at key developmental transitions" |
title_full |
Supplementary material from "Ocean pH fluctuations affect mussel larvae at key developmental transitions" |
title_fullStr |
Supplementary material from "Ocean pH fluctuations affect mussel larvae at key developmental transitions" |
title_full_unstemmed |
Supplementary material from "Ocean pH fluctuations affect mussel larvae at key developmental transitions" |
title_sort |
supplementary material from "ocean ph fluctuations affect mussel larvae at key developmental transitions" |
publisher |
Figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4325828.v1 https://figshare.com/collections/Supplementary_material_from_Ocean_pH_fluctuations_affect_mussel_larvae_at_key_developmental_transitions_/4325828/1 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1098/rspb.2018.2381 https://dx.doi.org/10.6084/m9.figshare.c.4325828 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4325828.v1 https://doi.org/10.1098/rspb.2018.2381 https://doi.org/10.6084/m9.figshare.c.4325828 |
_version_ |
1766156423603945472 |