Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification
Abstract Background Life in the ocean will increasingly have to contend with a complex matrix of concurrent shifts in environmental properties that impact their physiology and control their life histories. Rhodoliths are coralline red algae (Corallinales, Rhodophyta) that are photosynthesizers, calc...
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ftdatacite:10.6084/m9.figshare.c.4242977 2023-05-15T17:50:49+02:00 Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification Cavalcanti, Giselle Shukla, Priya Morris, Megan Bรกrbara Ribeiro Foley, Mariah Doane, Michael Cristiane Thompson Edwards, Matthew Dinsdale, Elizabeth Thompson, Fabiano 2018 https://dx.doi.org/10.6084/m9.figshare.c.4242977 https://figshare.com/collections/Rhodoliths_holobionts_in_a_changing_ocean_host-microbes_interactions_mediate_coralline_algae_resilience_under_ocean_acidification/4242977 unknown Figshare https://dx.doi.org/10.1186/s12864-018-5064-4 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4242977 https://doi.org/10.1186/s12864-018-5064-4 2021-11-05T12:55:41Z Abstract Background Life in the ocean will increasingly have to contend with a complex matrix of concurrent shifts in environmental properties that impact their physiology and control their life histories. Rhodoliths are coralline red algae (Corallinales, Rhodophyta) that are photosynthesizers, calcifiers, and ecosystem engineers and therefore represent important targets for ocean acidification (OA) research. Here, we exposed live rhodoliths to near-future OA conditions to investigate responses in their photosynthetic capacity, calcium carbonate production, and associated microbiome using carbon uptake, decalcification assays, and whole genome shotgun sequencing metagenomic analysis, respectively. The results from our live rhodolith assays were compared to similar manipulations on dead rhodolith (calcareous skeleton) biofilms and water column microbial communities, thereby enabling the assessment of host-microbiome interaction under climate-driven environmental perturbations. Results Under high pCO2 conditions, live rhodoliths exhibited positive physiological responses, i.e. increased photosynthetic activity, and no calcium carbonate biomass loss over time. Further, whereas the microbiome associated with live rhodoliths remained stable and resembled a healthy holobiont, the microbial community associated with the water column changed after exposure to elevated pCO2. Conclusions Our results suggest that a tightly regulated microbial-host interaction, as evidenced by the stability of the rhodolith microbiome recorded here under OA-like conditions, is important for host resilience to environmental stress. This study extends the scarce comprehension of microbes associated with rhodolith beds and their reaction to increased pCO2, providing a more comprehensive approach to OA studies by assessing the host holobiont. Article in Journal/Newspaper Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry |
spellingShingle |
Biochemistry Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Cavalcanti, Giselle Shukla, Priya Morris, Megan Bรกrbara Ribeiro Foley, Mariah Doane, Michael Cristiane Thompson Edwards, Matthew Dinsdale, Elizabeth Thompson, Fabiano Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
topic_facet |
Biochemistry Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry |
description |
Abstract Background Life in the ocean will increasingly have to contend with a complex matrix of concurrent shifts in environmental properties that impact their physiology and control their life histories. Rhodoliths are coralline red algae (Corallinales, Rhodophyta) that are photosynthesizers, calcifiers, and ecosystem engineers and therefore represent important targets for ocean acidification (OA) research. Here, we exposed live rhodoliths to near-future OA conditions to investigate responses in their photosynthetic capacity, calcium carbonate production, and associated microbiome using carbon uptake, decalcification assays, and whole genome shotgun sequencing metagenomic analysis, respectively. The results from our live rhodolith assays were compared to similar manipulations on dead rhodolith (calcareous skeleton) biofilms and water column microbial communities, thereby enabling the assessment of host-microbiome interaction under climate-driven environmental perturbations. Results Under high pCO2 conditions, live rhodoliths exhibited positive physiological responses, i.e. increased photosynthetic activity, and no calcium carbonate biomass loss over time. Further, whereas the microbiome associated with live rhodoliths remained stable and resembled a healthy holobiont, the microbial community associated with the water column changed after exposure to elevated pCO2. Conclusions Our results suggest that a tightly regulated microbial-host interaction, as evidenced by the stability of the rhodolith microbiome recorded here under OA-like conditions, is important for host resilience to environmental stress. This study extends the scarce comprehension of microbes associated with rhodolith beds and their reaction to increased pCO2, providing a more comprehensive approach to OA studies by assessing the host holobiont. |
format |
Article in Journal/Newspaper |
author |
Cavalcanti, Giselle Shukla, Priya Morris, Megan Bรกrbara Ribeiro Foley, Mariah Doane, Michael Cristiane Thompson Edwards, Matthew Dinsdale, Elizabeth Thompson, Fabiano |
author_facet |
Cavalcanti, Giselle Shukla, Priya Morris, Megan Bรกrbara Ribeiro Foley, Mariah Doane, Michael Cristiane Thompson Edwards, Matthew Dinsdale, Elizabeth Thompson, Fabiano |
author_sort |
Cavalcanti, Giselle |
title |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_short |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_full |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_fullStr |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_full_unstemmed |
Rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
title_sort |
rhodoliths holobionts in a changing ocean: host-microbes interactions mediate coralline algae resilience under ocean acidification |
publisher |
Figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4242977 https://figshare.com/collections/Rhodoliths_holobionts_in_a_changing_ocean_host-microbes_interactions_mediate_coralline_algae_resilience_under_ocean_acidification/4242977 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1186/s12864-018-5064-4 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4242977 https://doi.org/10.1186/s12864-018-5064-4 |
_version_ |
1766157720076943360 |