Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic
Abstract Intertidal macroalgae are exposed to many abiotic stress factors, and they must regularly react to changes in their environment. We used RNA-seq to describe how Porphyra umbilicalis (Rhodophyta) changes gene expression patterns to interact with different habitats. Tissue samples were taken...
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Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.4207796.v1 https://figshare.com/collections/Organism-environment_interactions_and_differential_gene_expression_patterns_among_open-coastal_and_estuarine_populations_of_Porphyra_umbilicalis_K_tzing_Rhodophyta_in_the_Northwest_Atlantic/4207796/1 |
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ftdatacite:10.6084/m9.figshare.c.4207796.v1 2023-05-15T17:45:33+02:00 Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic Eriksen, Renée Klein, Anita 2018 https://dx.doi.org/10.6084/m9.figshare.c.4207796.v1 https://figshare.com/collections/Organism-environment_interactions_and_differential_gene_expression_patterns_among_open-coastal_and_estuarine_populations_of_Porphyra_umbilicalis_K_tzing_Rhodophyta_in_the_Northwest_Atlantic/4207796/1 unknown Figshare https://dx.doi.org/10.1186/s41240-018-0103-2 https://dx.doi.org/10.6084/m9.figshare.c.4207796 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4207796.v1 https://doi.org/10.1186/s41240-018-0103-2 https://doi.org/10.6084/m9.figshare.c.4207796 2021-11-05T12:55:41Z Abstract Intertidal macroalgae are exposed to many abiotic stress factors, and they must regularly react to changes in their environment. We used RNA-seq to describe how Porphyra umbilicalis (Rhodophyta) changes gene expression patterns to interact with different habitats. Tissue samples were taken from a typical habitat along the open-coast of the Northwest Atlantic, as well as from a rare, atypical habitat in an estuarine tidal rapid environment. Differential gene expression analyses suggest that pathogic bacteria and viruses may be a significant factor influencing the transcriptome in the human-impacted estuarine environment, but the atypical habitat does not necessarily induce more stress in Porphyra umbilicalis growing there. We found genes related to nitrogen transport are over-expressed in tissue from the open-coastal site compared to those from the estuarine site, where environmental N levels approach hypertrophic levels. Low N levels impede growth, but high levels are toxic to cells, and we use qPCR to show this species regulates expression of a putative high-affinity NH4+ transporter under low and high N conditions. Differences in expression of this transporter in these habitats appear to be inherited from parent to offspring and have general implications for adaptation to habitat in other species that are capable of asexual reproduction, as well as more specific implications for this species’ use in aquaculture. Article in Journal/Newspaper Northwest Atlantic DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
spellingShingle |
Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Eriksen, Renée Klein, Anita Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic |
topic_facet |
Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
description |
Abstract Intertidal macroalgae are exposed to many abiotic stress factors, and they must regularly react to changes in their environment. We used RNA-seq to describe how Porphyra umbilicalis (Rhodophyta) changes gene expression patterns to interact with different habitats. Tissue samples were taken from a typical habitat along the open-coast of the Northwest Atlantic, as well as from a rare, atypical habitat in an estuarine tidal rapid environment. Differential gene expression analyses suggest that pathogic bacteria and viruses may be a significant factor influencing the transcriptome in the human-impacted estuarine environment, but the atypical habitat does not necessarily induce more stress in Porphyra umbilicalis growing there. We found genes related to nitrogen transport are over-expressed in tissue from the open-coastal site compared to those from the estuarine site, where environmental N levels approach hypertrophic levels. Low N levels impede growth, but high levels are toxic to cells, and we use qPCR to show this species regulates expression of a putative high-affinity NH4+ transporter under low and high N conditions. Differences in expression of this transporter in these habitats appear to be inherited from parent to offspring and have general implications for adaptation to habitat in other species that are capable of asexual reproduction, as well as more specific implications for this species’ use in aquaculture. |
format |
Article in Journal/Newspaper |
author |
Eriksen, Renée Klein, Anita |
author_facet |
Eriksen, Renée Klein, Anita |
author_sort |
Eriksen, Renée |
title |
Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic |
title_short |
Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic |
title_full |
Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic |
title_fullStr |
Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic |
title_full_unstemmed |
Organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of Porphyra umbilicalis Kützing (Rhodophyta) in the Northwest Atlantic |
title_sort |
organism-environment interactions and differential gene expression patterns among open-coastal and estuarine populations of porphyra umbilicalis kützing (rhodophyta) in the northwest atlantic |
publisher |
Figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4207796.v1 https://figshare.com/collections/Organism-environment_interactions_and_differential_gene_expression_patterns_among_open-coastal_and_estuarine_populations_of_Porphyra_umbilicalis_K_tzing_Rhodophyta_in_the_Northwest_Atlantic/4207796/1 |
genre |
Northwest Atlantic |
genre_facet |
Northwest Atlantic |
op_relation |
https://dx.doi.org/10.1186/s41240-018-0103-2 https://dx.doi.org/10.6084/m9.figshare.c.4207796 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4207796.v1 https://doi.org/10.1186/s41240-018-0103-2 https://doi.org/10.6084/m9.figshare.c.4207796 |
_version_ |
1766148653891715072 |