Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology
Abstract Background Collecting and studying live sperm is central to many important fields of biology. Yet, a simple method to collect live sperm is lacking in wild seabird species. Here, we describe a non-invasive method to collect viable sperm samples based on a simple massage technique applied to...
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ftdatacite:10.6084/m9.figshare.c.4165712 2023-05-15T17:05:14+02:00 Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology Humann-Guilleminot, Ségolène Blévin, Pierre Azou-Barré, Antonin Yacoumas, Agathe Gabrielsen, Geir Chastel, Olivier Helfenstein, Fabrice 2018 https://dx.doi.org/10.6084/m9.figshare.c.4165712 https://figshare.com/collections/Sperm_collection_in_Black-legged_Kittiwakes_and_characterization_of_sperm_velocity_and_morphology/4165712 unknown Figshare https://dx.doi.org/10.1186/s40657-018-0117-6 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Cell Biology Molecular Biology Physiology FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Developmental Biology Cancer Inorganic Chemistry FOS Chemical sciences 60506 Virology Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4165712 https://doi.org/10.1186/s40657-018-0117-6 2021-11-05T12:55:41Z Abstract Background Collecting and studying live sperm is central to many important fields of biology. Yet, a simple method to collect live sperm is lacking in wild seabird species. Here, we describe a non-invasive method to collect viable sperm samples based on a simple massage technique applied to male Black-legged Kittiwakes (Rissa tridactyla). Methods We studied a colony breeding at Kongsfjorden, Svalbard and successfully obtained sperm samples from 32 males. With a subset of samples (n = 12 males), we compared the suitability of several extenders (0.9% NaCl, PBS, Earle’s balance salt solution, Dulbecco’s modified Eagle medium) in maintaining sperm alive long enough for analyses. With another 18 ejaculates, we conducted computer assisted sperm analyses using the CASA plugin for ImageJ. We provide details about the settings to be used for such analyses. Lastly, droplets from 20 ejaculates were smeared on glass slides and preserved with formalin to characterize sperm morphology in terms of total sperm length, sperm head length, midpiece length and flagellum length, and percentage of abnormal sperm. Results With this method and under field conditions, we were able to obtain sufficient amounts of live sperm to assess traits related to sperm quality (e.g. sperm morphology, percentage of motile sperm, sperm velocity). We found that two extenders, Earle’s balanced salt solution and Dulbecco modified Eagle’s medium, yielded similarly good results. Additionally, we investigated whether specific behaviours were associated with successful sperm collection and whether sperm collection success depended on how long before laying sperm collection was attempted. Finally, we provide mean values for sperm morphology, sperm swimming ability and percentage of motile sperm, which may prove useful for future comparative analyses, and we report high levels of sperm abnormality and within-ejaculate variation in sperm morphology. Conclusions We discuss the high percentage of abnormal sperm and high within-ejaculate variation in sperm morphology in light of sperm competition theory and conclude that these figures are likely due to relaxed post-copulatory sexual selection, kittiwakes being strictly monogamous. Finally, we suggest that this method could be applied to other seabird species sharing similar ecology. Article in Journal/Newspaper Kongsfjord* Kongsfjorden rissa tridactyla Svalbard DataCite Metadata Store (German National Library of Science and Technology) Svalbard |
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collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Cell Biology Molecular Biology Physiology FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Developmental Biology Cancer Inorganic Chemistry FOS Chemical sciences 60506 Virology |
spellingShingle |
Cell Biology Molecular Biology Physiology FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Developmental Biology Cancer Inorganic Chemistry FOS Chemical sciences 60506 Virology Humann-Guilleminot, Ségolène Blévin, Pierre Azou-Barré, Antonin Yacoumas, Agathe Gabrielsen, Geir Chastel, Olivier Helfenstein, Fabrice Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology |
topic_facet |
Cell Biology Molecular Biology Physiology FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Developmental Biology Cancer Inorganic Chemistry FOS Chemical sciences 60506 Virology |
description |
Abstract Background Collecting and studying live sperm is central to many important fields of biology. Yet, a simple method to collect live sperm is lacking in wild seabird species. Here, we describe a non-invasive method to collect viable sperm samples based on a simple massage technique applied to male Black-legged Kittiwakes (Rissa tridactyla). Methods We studied a colony breeding at Kongsfjorden, Svalbard and successfully obtained sperm samples from 32 males. With a subset of samples (n = 12 males), we compared the suitability of several extenders (0.9% NaCl, PBS, Earle’s balance salt solution, Dulbecco’s modified Eagle medium) in maintaining sperm alive long enough for analyses. With another 18 ejaculates, we conducted computer assisted sperm analyses using the CASA plugin for ImageJ. We provide details about the settings to be used for such analyses. Lastly, droplets from 20 ejaculates were smeared on glass slides and preserved with formalin to characterize sperm morphology in terms of total sperm length, sperm head length, midpiece length and flagellum length, and percentage of abnormal sperm. Results With this method and under field conditions, we were able to obtain sufficient amounts of live sperm to assess traits related to sperm quality (e.g. sperm morphology, percentage of motile sperm, sperm velocity). We found that two extenders, Earle’s balanced salt solution and Dulbecco modified Eagle’s medium, yielded similarly good results. Additionally, we investigated whether specific behaviours were associated with successful sperm collection and whether sperm collection success depended on how long before laying sperm collection was attempted. Finally, we provide mean values for sperm morphology, sperm swimming ability and percentage of motile sperm, which may prove useful for future comparative analyses, and we report high levels of sperm abnormality and within-ejaculate variation in sperm morphology. Conclusions We discuss the high percentage of abnormal sperm and high within-ejaculate variation in sperm morphology in light of sperm competition theory and conclude that these figures are likely due to relaxed post-copulatory sexual selection, kittiwakes being strictly monogamous. Finally, we suggest that this method could be applied to other seabird species sharing similar ecology. |
format |
Article in Journal/Newspaper |
author |
Humann-Guilleminot, Ségolène Blévin, Pierre Azou-Barré, Antonin Yacoumas, Agathe Gabrielsen, Geir Chastel, Olivier Helfenstein, Fabrice |
author_facet |
Humann-Guilleminot, Ségolène Blévin, Pierre Azou-Barré, Antonin Yacoumas, Agathe Gabrielsen, Geir Chastel, Olivier Helfenstein, Fabrice |
author_sort |
Humann-Guilleminot, Ségolène |
title |
Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology |
title_short |
Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology |
title_full |
Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology |
title_fullStr |
Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology |
title_full_unstemmed |
Sperm collection in Black-legged Kittiwakes and characterization of sperm velocity and morphology |
title_sort |
sperm collection in black-legged kittiwakes and characterization of sperm velocity and morphology |
publisher |
Figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4165712 https://figshare.com/collections/Sperm_collection_in_Black-legged_Kittiwakes_and_characterization_of_sperm_velocity_and_morphology/4165712 |
geographic |
Svalbard |
geographic_facet |
Svalbard |
genre |
Kongsfjord* Kongsfjorden rissa tridactyla Svalbard |
genre_facet |
Kongsfjord* Kongsfjorden rissa tridactyla Svalbard |
op_relation |
https://dx.doi.org/10.1186/s40657-018-0117-6 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4165712 https://doi.org/10.1186/s40657-018-0117-6 |
_version_ |
1766059641451577344 |