Metagenomic analysis of basal ice from an Alaskan glacier ...
Abstract Background Glaciers cover ~â 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is v...
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ftdatacite:10.6084/m9.figshare.c.4155590.v1 2024-04-28T08:19:43+00:00 Metagenomic analysis of basal ice from an Alaskan glacier ... Kayani, Masood ur Rehman Doyle, Shawn Sangwan, Naseer Wang, Guanqun Gilbert, Jack Christner, Brent Zhu, Ting 2018 https://dx.doi.org/10.6084/m9.figshare.c.4155590.v1 https://springernature.figshare.com/collections/Metagenomic_analysis_of_basal_ice_from_an_Alaskan_glacier/4155590/1 unknown figshare https://dx.doi.org/10.6084/m9.figshare.c.4155590 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Microbiology FOS Biological sciences Genetics Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4155590.v110.6084/m9.figshare.c.4155590 2024-04-02T11:55:14Z Abstract Background Glaciers cover ~â 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is very limited. Here, we provide insights into the microbial lifestyle present at the base of the Matanuska Glacier, Alaska. Results DNA and RNA were extracted from samples of the Matanuska Glacier basal ice. Using Illumina MiSeq and HiSeq sequencing, we investigated the microbial diversity with the metagenomic shotgun reads and 16S ribosomal RNA data. We further assembled 9 partial and draft bacterial genomes from the metagenomic assembly, and identified key metabolic pathways such as sulfur oxidation and nitrification. Collectively, our analyses suggest a prevalence of lithotrophic and heterotrophic metabolisms in the subglacial microbiome. Conclusion Our results present the first metagenomic assembly and bacterial ... Article in Journal/Newspaper glacier glaciers Alaska DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
unknown |
topic |
Microbiology FOS Biological sciences Genetics Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
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Microbiology FOS Biological sciences Genetics Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Kayani, Masood ur Rehman Doyle, Shawn Sangwan, Naseer Wang, Guanqun Gilbert, Jack Christner, Brent Zhu, Ting Metagenomic analysis of basal ice from an Alaskan glacier ... |
topic_facet |
Microbiology FOS Biological sciences Genetics Environmental Sciences not elsewhere classified Ecology Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences |
description |
Abstract Background Glaciers cover ~â 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is very limited. Here, we provide insights into the microbial lifestyle present at the base of the Matanuska Glacier, Alaska. Results DNA and RNA were extracted from samples of the Matanuska Glacier basal ice. Using Illumina MiSeq and HiSeq sequencing, we investigated the microbial diversity with the metagenomic shotgun reads and 16S ribosomal RNA data. We further assembled 9 partial and draft bacterial genomes from the metagenomic assembly, and identified key metabolic pathways such as sulfur oxidation and nitrification. Collectively, our analyses suggest a prevalence of lithotrophic and heterotrophic metabolisms in the subglacial microbiome. Conclusion Our results present the first metagenomic assembly and bacterial ... |
format |
Article in Journal/Newspaper |
author |
Kayani, Masood ur Rehman Doyle, Shawn Sangwan, Naseer Wang, Guanqun Gilbert, Jack Christner, Brent Zhu, Ting |
author_facet |
Kayani, Masood ur Rehman Doyle, Shawn Sangwan, Naseer Wang, Guanqun Gilbert, Jack Christner, Brent Zhu, Ting |
author_sort |
Kayani, Masood ur Rehman |
title |
Metagenomic analysis of basal ice from an Alaskan glacier ... |
title_short |
Metagenomic analysis of basal ice from an Alaskan glacier ... |
title_full |
Metagenomic analysis of basal ice from an Alaskan glacier ... |
title_fullStr |
Metagenomic analysis of basal ice from an Alaskan glacier ... |
title_full_unstemmed |
Metagenomic analysis of basal ice from an Alaskan glacier ... |
title_sort |
metagenomic analysis of basal ice from an alaskan glacier ... |
publisher |
figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4155590.v1 https://springernature.figshare.com/collections/Metagenomic_analysis_of_basal_ice_from_an_Alaskan_glacier/4155590/1 |
genre |
glacier glaciers Alaska |
genre_facet |
glacier glaciers Alaska |
op_relation |
https://dx.doi.org/10.6084/m9.figshare.c.4155590 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4155590.v110.6084/m9.figshare.c.4155590 |
_version_ |
1797583072771702784 |