High species diversity of trichostrongyle parasite communities within and between Western Canadian commercial and conservation bison herds revealed by nemabiome metabarcoding
Abstract Background Many trichostrongylid nematode species are reported to infect bison, some of which are major causes of disase and production loss in North American bison herds. However, there is little information on the species distribution and relative abundance of these parasites in either co...
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Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.4101596 https://figshare.com/collections/High_species_diversity_of_trichostrongyle_parasite_communities_within_and_between_Western_Canadian_commercial_and_conservation_bison_herds_revealed_by_nemabiome_metabarcoding/4101596 |
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ftdatacite:10.6084/m9.figshare.c.4101596 2023-05-15T15:44:48+02:00 High species diversity of trichostrongyle parasite communities within and between Western Canadian commercial and conservation bison herds revealed by nemabiome metabarcoding Avramenko, Russell Bras, Ana Redman, Elizabeth Woodbury, Murray Wagner, Brent Shury, Todd Liccioli, Stefano M. Windeyer Gilleard, John 2018 https://dx.doi.org/10.6084/m9.figshare.c.4101596 https://figshare.com/collections/High_species_diversity_of_trichostrongyle_parasite_communities_within_and_between_Western_Canadian_commercial_and_conservation_bison_herds_revealed_by_nemabiome_metabarcoding/4101596 unknown Figshare https://dx.doi.org/10.1186/s13071-018-2880-y CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Medicine Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4101596 https://doi.org/10.1186/s13071-018-2880-y 2021-11-05T12:55:41Z Abstract Background Many trichostrongylid nematode species are reported to infect bison, some of which are major causes of disase and production loss in North American bison herds. However, there is little information on the species distribution and relative abundance of these parasites in either commercial or conservation herds. This is largely because trichostrongylid nematode species cannot be distinguished by visual microscopic examination of eggs present in feces. Consequently, we have applied ITS2 rDNA nemabiome metabarcoding to describe the trichostrongyle parasite species diversity in 58 bison production groups derived from 38 commercial North American plains bison (Bison bison bison) herds from across western Canada, and two bison conservation herds located in Elk Island National Park (EINP) [plains bison and wood bison (Bison bison athabascae)] and one in Grasslands National Park (GNP) (plains bison). Results We report much higher infection intensities and parasite species diversity in commercial bison herds than previously reported in beef cattle herds grazing similar latitudes. Predominant trichostrongyle parasite species in western Canadian commercial bison herds are those commonly associated with Canadian cattle, with Ostertagia ostertagi being the most abundant followed by Cooperia oncophora. Combined with high fecal egg counts in many herds, this is consistent with significant clinical and production-limiting gastrointestinal parasitism in western Canadian bison herds. However, Haemonchus placei was the most abundant species in five of the production groups. This is both surprising and important, as this highly pathogenic blood-feeding parasite has not been reported at such abundance, in any livestock species, at such northerly latitudes. The presence of Trichostrongylus axei as the most abundant parasite in four herds is also unusual, relative to cattle. There were striking differences in parasite communities between the EINP and commercial bison herds. Most notably, Orloffia bisonis was the predominant species in the wood bison herd despite being found at only low levels in all other herds surveyed. Conclusions This study represents the most comprehensive description of parasite communities in North American bison to date and illustrates the power of deep amplicon sequencing as a tool to study species diversity in gastrointestinal nematode communities. Article in Journal/Newspaper Bison bison athabascae Wood Bison Bison bison bison Plains Bison DataCite Metadata Store (German National Library of Science and Technology) Canada |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Medicine Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology |
spellingShingle |
Medicine Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Avramenko, Russell Bras, Ana Redman, Elizabeth Woodbury, Murray Wagner, Brent Shury, Todd Liccioli, Stefano M. Windeyer Gilleard, John High species diversity of trichostrongyle parasite communities within and between Western Canadian commercial and conservation bison herds revealed by nemabiome metabarcoding |
topic_facet |
Medicine Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology |
description |
Abstract Background Many trichostrongylid nematode species are reported to infect bison, some of which are major causes of disase and production loss in North American bison herds. However, there is little information on the species distribution and relative abundance of these parasites in either commercial or conservation herds. This is largely because trichostrongylid nematode species cannot be distinguished by visual microscopic examination of eggs present in feces. Consequently, we have applied ITS2 rDNA nemabiome metabarcoding to describe the trichostrongyle parasite species diversity in 58 bison production groups derived from 38 commercial North American plains bison (Bison bison bison) herds from across western Canada, and two bison conservation herds located in Elk Island National Park (EINP) [plains bison and wood bison (Bison bison athabascae)] and one in Grasslands National Park (GNP) (plains bison). Results We report much higher infection intensities and parasite species diversity in commercial bison herds than previously reported in beef cattle herds grazing similar latitudes. Predominant trichostrongyle parasite species in western Canadian commercial bison herds are those commonly associated with Canadian cattle, with Ostertagia ostertagi being the most abundant followed by Cooperia oncophora. Combined with high fecal egg counts in many herds, this is consistent with significant clinical and production-limiting gastrointestinal parasitism in western Canadian bison herds. However, Haemonchus placei was the most abundant species in five of the production groups. This is both surprising and important, as this highly pathogenic blood-feeding parasite has not been reported at such abundance, in any livestock species, at such northerly latitudes. The presence of Trichostrongylus axei as the most abundant parasite in four herds is also unusual, relative to cattle. There were striking differences in parasite communities between the EINP and commercial bison herds. Most notably, Orloffia bisonis was the predominant species in the wood bison herd despite being found at only low levels in all other herds surveyed. Conclusions This study represents the most comprehensive description of parasite communities in North American bison to date and illustrates the power of deep amplicon sequencing as a tool to study species diversity in gastrointestinal nematode communities. |
format |
Article in Journal/Newspaper |
author |
Avramenko, Russell Bras, Ana Redman, Elizabeth Woodbury, Murray Wagner, Brent Shury, Todd Liccioli, Stefano M. Windeyer Gilleard, John |
author_facet |
Avramenko, Russell Bras, Ana Redman, Elizabeth Woodbury, Murray Wagner, Brent Shury, Todd Liccioli, Stefano M. Windeyer Gilleard, John |
author_sort |
Avramenko, Russell |
title |
High species diversity of trichostrongyle parasite communities within and between Western Canadian commercial and conservation bison herds revealed by nemabiome metabarcoding |
title_short |
High species diversity of trichostrongyle parasite communities within and between Western Canadian commercial and conservation bison herds revealed by nemabiome metabarcoding |
title_full |
High species diversity of trichostrongyle parasite communities within and between Western Canadian commercial and conservation bison herds revealed by nemabiome metabarcoding |
title_fullStr |
High species diversity of trichostrongyle parasite communities within and between Western Canadian commercial and conservation bison herds revealed by nemabiome metabarcoding |
title_full_unstemmed |
High species diversity of trichostrongyle parasite communities within and between Western Canadian commercial and conservation bison herds revealed by nemabiome metabarcoding |
title_sort |
high species diversity of trichostrongyle parasite communities within and between western canadian commercial and conservation bison herds revealed by nemabiome metabarcoding |
publisher |
Figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4101596 https://figshare.com/collections/High_species_diversity_of_trichostrongyle_parasite_communities_within_and_between_Western_Canadian_commercial_and_conservation_bison_herds_revealed_by_nemabiome_metabarcoding/4101596 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Bison bison athabascae Wood Bison Bison bison bison Plains Bison |
genre_facet |
Bison bison athabascae Wood Bison Bison bison bison Plains Bison |
op_relation |
https://dx.doi.org/10.1186/s13071-018-2880-y |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4101596 https://doi.org/10.1186/s13071-018-2880-y |
_version_ |
1766379165461774336 |