The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses
Abstract Biological nitrogen fixation is an important source of bioavailable nitrogen in Sphagnum dominated peatlands. Sphagnum mosses harbor a diverse microbiome including nitrogen-fixing and methane (CH4) oxidizing bacteria. The inhibitory effect of oxygen on microbial nitrogen fixation is documen...
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ftdatacite:10.6084/m9.figshare.c.4091075 2023-05-15T18:28:17+02:00 The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses Kox, Martine Aalto, Sanni Penttilä, Timo Ettwig, Katharina Jetten, Mike Kessel, Maartje 2018 https://dx.doi.org/10.6084/m9.figshare.c.4091075 https://figshare.com/collections/The_influence_of_oxygen_and_methane_on_nitrogen_fixation_in_subarctic_Sphagnum_mosses/4091075 unknown Figshare https://dx.doi.org/10.1186/s13568-018-0607-2 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Plant Biology 60506 Virology Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4091075 https://doi.org/10.1186/s13568-018-0607-2 2021-11-05T12:55:41Z Abstract Biological nitrogen fixation is an important source of bioavailable nitrogen in Sphagnum dominated peatlands. Sphagnum mosses harbor a diverse microbiome including nitrogen-fixing and methane (CH4) oxidizing bacteria. The inhibitory effect of oxygen on microbial nitrogen fixation is documented for many bacteria. However, the role of nitrogen-fixing methanotrophs in nitrogen supply to Sphagnum peat mosses is not well explored. Here, we investigated the role of both oxygen and methane on nitrogen fixation in subarctic Sphagnum peat mosses. Five species of Sphagnum mosses were sampled from two mesotrophic and three oligotrophic sites within the Lakkasuo peatland in Orivesi, central Finland. Mosses were incubated under either ambient or low oxygen conditions in the presence or absence of methane. Stable isotope activity assays revealed considerable nitrogen-fixing and methane-assimilating rates at all sites (1.4 ± 0.2 µmol 15N–N2 g−1 DW day−1 and 12.0 ± 1.1 µmol 13C–CH4 g−1 DW day−1, respectively). Addition of methane did not stimulate incorporation of 15N-nitrogen into biomass, whereas oxygen depletion increased the activity of the nitrogen-fixing community. Analysis of the 16S rRNA genes at the bacterial community level showed a very diverse microbiome that was dominated by Alphaproteobacteria in all sites. Bona fide methane-oxidizing taxa were not very abundant (relative abundance less than 0.1%). Based on our results we conclude that methanotrophs did not contribute significantly to nitrogen fixation in the investigated peatlands. Article in Journal/Newspaper Subarctic DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Plant Biology 60506 Virology |
spellingShingle |
Biochemistry Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Plant Biology 60506 Virology Kox, Martine Aalto, Sanni Penttilä, Timo Ettwig, Katharina Jetten, Mike Kessel, Maartje The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
topic_facet |
Biochemistry Microbiology FOS Biological sciences 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Plant Biology 60506 Virology |
description |
Abstract Biological nitrogen fixation is an important source of bioavailable nitrogen in Sphagnum dominated peatlands. Sphagnum mosses harbor a diverse microbiome including nitrogen-fixing and methane (CH4) oxidizing bacteria. The inhibitory effect of oxygen on microbial nitrogen fixation is documented for many bacteria. However, the role of nitrogen-fixing methanotrophs in nitrogen supply to Sphagnum peat mosses is not well explored. Here, we investigated the role of both oxygen and methane on nitrogen fixation in subarctic Sphagnum peat mosses. Five species of Sphagnum mosses were sampled from two mesotrophic and three oligotrophic sites within the Lakkasuo peatland in Orivesi, central Finland. Mosses were incubated under either ambient or low oxygen conditions in the presence or absence of methane. Stable isotope activity assays revealed considerable nitrogen-fixing and methane-assimilating rates at all sites (1.4 ± 0.2 µmol 15N–N2 g−1 DW day−1 and 12.0 ± 1.1 µmol 13C–CH4 g−1 DW day−1, respectively). Addition of methane did not stimulate incorporation of 15N-nitrogen into biomass, whereas oxygen depletion increased the activity of the nitrogen-fixing community. Analysis of the 16S rRNA genes at the bacterial community level showed a very diverse microbiome that was dominated by Alphaproteobacteria in all sites. Bona fide methane-oxidizing taxa were not very abundant (relative abundance less than 0.1%). Based on our results we conclude that methanotrophs did not contribute significantly to nitrogen fixation in the investigated peatlands. |
format |
Article in Journal/Newspaper |
author |
Kox, Martine Aalto, Sanni Penttilä, Timo Ettwig, Katharina Jetten, Mike Kessel, Maartje |
author_facet |
Kox, Martine Aalto, Sanni Penttilä, Timo Ettwig, Katharina Jetten, Mike Kessel, Maartje |
author_sort |
Kox, Martine |
title |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_short |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_full |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_fullStr |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_full_unstemmed |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses |
title_sort |
influence of oxygen and methane on nitrogen fixation in subarctic sphagnum mosses |
publisher |
Figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4091075 https://figshare.com/collections/The_influence_of_oxygen_and_methane_on_nitrogen_fixation_in_subarctic_Sphagnum_mosses/4091075 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_relation |
https://dx.doi.org/10.1186/s13568-018-0607-2 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4091075 https://doi.org/10.1186/s13568-018-0607-2 |
_version_ |
1766210702365687808 |