Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil
Abstract Background The expansion of offshore oil exploration increases the risk of marine species being exposed to oil pollution in currently pristine areas. The adverse effects of oil exposure through toxic properties of polycyclic aromatic hydrocarbons (PAHs) have been well studied in Atlantic co...
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ftdatacite:10.6084/m9.figshare.c.4056218.v1 2023-05-15T15:27:15+02:00 Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil Bagi, Andrea Riiser, Even Molland, Hilde Star, Bastiaan Haverkamp, Thomas Sydnes, Magne Pampanin, Daniela 2018 https://dx.doi.org/10.6084/m9.figshare.c.4056218.v1 https://figshare.com/collections/Gastrointestinal_microbial_community_changes_in_Atlantic_cod_Gadus_morhua_exposed_to_crude_oil/4056218/1 unknown Figshare https://dx.doi.org/10.1186/s12866-018-1171-2 https://dx.doi.org/10.6084/m9.figshare.c.4056218 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4056218.v1 https://doi.org/10.1186/s12866-018-1171-2 https://doi.org/10.6084/m9.figshare.c.4056218 2021-11-05T12:55:41Z Abstract Background The expansion of offshore oil exploration increases the risk of marine species being exposed to oil pollution in currently pristine areas. The adverse effects of oil exposure through toxic properties of polycyclic aromatic hydrocarbons (PAHs) have been well studied in Atlantic cod (Gadus morhua). Nevertheless, the fate of conjugated metabolites in the intestinal tract and their effect on the diversity of intestinal microbial community in fish is less understood. Here, we investigated the intestinal microbial community composition of Atlantic cod after 28 days of exposure to crude oil (concentration range 0.0–0.1 mg/L). Results Analysis of PAH metabolites in bile samples confirmed that uptake and biotransformation of oil compounds occurred as a result of the exposure. Various evidence for altered microbial communities was found in fish exposed to high (0.1 mg/L) and medium (0.05 mg/L) concentrations of oil when compared to fish exposed to low oil concentration (0.01 mg/L) or no oil (control). First, altered banding patterns were observed on denaturing gradient gel electrophoresis for samples pooled from each treatment group. Secondly, based on 16S rRNA sequences, higher levels of oil exposure were associated with a loss of overall diversity of the gut microbial communities. Furthermore, 8 operational taxonomic units (OTUs) were found to have significantly different relative abundances in samples from fishes exposed to high and medium oil concentrations when compared to samples from the control group and low oil concentration. Among these, only one OTU, a Deferribacterales, had increased relative abundance in samples from fish exposed to high oil concentration. Conclusions The results presented herein contribute to a better understanding of the effects of oil contamination on the gut microbial community changes in fish and highlight the importance of further studies into the area. Our findings suggest that increased relative abundance of bacteria belonging to the order Deferribacterales may be indicative of exposure to oil at concentrations higher than 0.05 mg/L. Article in Journal/Newspaper atlantic cod Gadus morhua DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry |
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Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Bagi, Andrea Riiser, Even Molland, Hilde Star, Bastiaan Haverkamp, Thomas Sydnes, Magne Pampanin, Daniela Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil |
topic_facet |
Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry |
description |
Abstract Background The expansion of offshore oil exploration increases the risk of marine species being exposed to oil pollution in currently pristine areas. The adverse effects of oil exposure through toxic properties of polycyclic aromatic hydrocarbons (PAHs) have been well studied in Atlantic cod (Gadus morhua). Nevertheless, the fate of conjugated metabolites in the intestinal tract and their effect on the diversity of intestinal microbial community in fish is less understood. Here, we investigated the intestinal microbial community composition of Atlantic cod after 28 days of exposure to crude oil (concentration range 0.0–0.1 mg/L). Results Analysis of PAH metabolites in bile samples confirmed that uptake and biotransformation of oil compounds occurred as a result of the exposure. Various evidence for altered microbial communities was found in fish exposed to high (0.1 mg/L) and medium (0.05 mg/L) concentrations of oil when compared to fish exposed to low oil concentration (0.01 mg/L) or no oil (control). First, altered banding patterns were observed on denaturing gradient gel electrophoresis for samples pooled from each treatment group. Secondly, based on 16S rRNA sequences, higher levels of oil exposure were associated with a loss of overall diversity of the gut microbial communities. Furthermore, 8 operational taxonomic units (OTUs) were found to have significantly different relative abundances in samples from fishes exposed to high and medium oil concentrations when compared to samples from the control group and low oil concentration. Among these, only one OTU, a Deferribacterales, had increased relative abundance in samples from fish exposed to high oil concentration. Conclusions The results presented herein contribute to a better understanding of the effects of oil contamination on the gut microbial community changes in fish and highlight the importance of further studies into the area. Our findings suggest that increased relative abundance of bacteria belonging to the order Deferribacterales may be indicative of exposure to oil at concentrations higher than 0.05 mg/L. |
format |
Article in Journal/Newspaper |
author |
Bagi, Andrea Riiser, Even Molland, Hilde Star, Bastiaan Haverkamp, Thomas Sydnes, Magne Pampanin, Daniela |
author_facet |
Bagi, Andrea Riiser, Even Molland, Hilde Star, Bastiaan Haverkamp, Thomas Sydnes, Magne Pampanin, Daniela |
author_sort |
Bagi, Andrea |
title |
Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil |
title_short |
Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil |
title_full |
Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil |
title_fullStr |
Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil |
title_full_unstemmed |
Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil |
title_sort |
gastrointestinal microbial community changes in atlantic cod (gadus morhua) exposed to crude oil |
publisher |
Figshare |
publishDate |
2018 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.4056218.v1 https://figshare.com/collections/Gastrointestinal_microbial_community_changes_in_Atlantic_cod_Gadus_morhua_exposed_to_crude_oil/4056218/1 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
https://dx.doi.org/10.1186/s12866-018-1171-2 https://dx.doi.org/10.6084/m9.figshare.c.4056218 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.4056218.v1 https://doi.org/10.1186/s12866-018-1171-2 https://doi.org/10.6084/m9.figshare.c.4056218 |
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1766357703111737344 |