Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil

Abstract Background The expansion of offshore oil exploration increases the risk of marine species being exposed to oil pollution in currently pristine areas. The adverse effects of oil exposure through toxic properties of polycyclic aromatic hydrocarbons (PAHs) have been well studied in Atlantic co...

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Main Authors: Bagi, Andrea, Riiser, Even, Molland, Hilde, Star, Bastiaan, Haverkamp, Thomas, Sydnes, Magne, Pampanin, Daniela
Format: Article in Journal/Newspaper
Language:unknown
Published: Figshare 2018
Subjects:
Online Access:https://dx.doi.org/10.6084/m9.figshare.c.4056218.v1
https://figshare.com/collections/Gastrointestinal_microbial_community_changes_in_Atlantic_cod_Gadus_morhua_exposed_to_crude_oil/4056218/1
id ftdatacite:10.6084/m9.figshare.c.4056218.v1
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spelling ftdatacite:10.6084/m9.figshare.c.4056218.v1 2023-05-15T15:27:15+02:00 Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil Bagi, Andrea Riiser, Even Molland, Hilde Star, Bastiaan Haverkamp, Thomas Sydnes, Magne Pampanin, Daniela 2018 https://dx.doi.org/10.6084/m9.figshare.c.4056218.v1 https://figshare.com/collections/Gastrointestinal_microbial_community_changes_in_Atlantic_cod_Gadus_morhua_exposed_to_crude_oil/4056218/1 unknown Figshare https://dx.doi.org/10.1186/s12866-018-1171-2 https://dx.doi.org/10.6084/m9.figshare.c.4056218 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology FOS Biological sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Collection article 2018 ftdatacite https://doi.org/10.6084/m9.figshare.c.4056218.v1 https://doi.org/10.1186/s12866-018-1171-2 https://doi.org/10.6084/m9.figshare.c.4056218 2021-11-05T12:55:41Z Abstract Background The expansion of offshore oil exploration increases the risk of marine species being exposed to oil pollution in currently pristine areas. The adverse effects of oil exposure through toxic properties of polycyclic aromatic hydrocarbons (PAHs) have been well studied in Atlantic cod (Gadus morhua). Nevertheless, the fate of conjugated metabolites in the intestinal tract and their effect on the diversity of intestinal microbial community in fish is less understood. Here, we investigated the intestinal microbial community composition of Atlantic cod after 28 days of exposure to crude oil (concentration range 0.0–0.1 mg/L). Results Analysis of PAH metabolites in bile samples confirmed that uptake and biotransformation of oil compounds occurred as a result of the exposure. Various evidence for altered microbial communities was found in fish exposed to high (0.1 mg/L) and medium (0.05 mg/L) concentrations of oil when compared to fish exposed to low oil concentration (0.01 mg/L) or no oil (control). First, altered banding patterns were observed on denaturing gradient gel electrophoresis for samples pooled from each treatment group. Secondly, based on 16S rRNA sequences, higher levels of oil exposure were associated with a loss of overall diversity of the gut microbial communities. Furthermore, 8 operational taxonomic units (OTUs) were found to have significantly different relative abundances in samples from fishes exposed to high and medium oil concentrations when compared to samples from the control group and low oil concentration. Among these, only one OTU, a Deferribacterales, had increased relative abundance in samples from fish exposed to high oil concentration. Conclusions The results presented herein contribute to a better understanding of the effects of oil contamination on the gut microbial community changes in fish and highlight the importance of further studies into the area. Our findings suggest that increased relative abundance of bacteria belonging to the order Deferribacterales may be indicative of exposure to oil at concentrations higher than 0.05 mg/L. Article in Journal/Newspaper atlantic cod Gadus morhua DataCite Metadata Store (German National Library of Science and Technology)
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language unknown
topic Biotechnology
59999 Environmental Sciences not elsewhere classified
FOS Earth and related environmental sciences
39999 Chemical Sciences not elsewhere classified
FOS Chemical sciences
Ecology
FOS Biological sciences
69999 Biological Sciences not elsewhere classified
Inorganic Chemistry
spellingShingle Biotechnology
59999 Environmental Sciences not elsewhere classified
FOS Earth and related environmental sciences
39999 Chemical Sciences not elsewhere classified
FOS Chemical sciences
Ecology
FOS Biological sciences
69999 Biological Sciences not elsewhere classified
Inorganic Chemistry
Bagi, Andrea
Riiser, Even
Molland, Hilde
Star, Bastiaan
Haverkamp, Thomas
Sydnes, Magne
Pampanin, Daniela
Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil
topic_facet Biotechnology
59999 Environmental Sciences not elsewhere classified
FOS Earth and related environmental sciences
39999 Chemical Sciences not elsewhere classified
FOS Chemical sciences
Ecology
FOS Biological sciences
69999 Biological Sciences not elsewhere classified
Inorganic Chemistry
description Abstract Background The expansion of offshore oil exploration increases the risk of marine species being exposed to oil pollution in currently pristine areas. The adverse effects of oil exposure through toxic properties of polycyclic aromatic hydrocarbons (PAHs) have been well studied in Atlantic cod (Gadus morhua). Nevertheless, the fate of conjugated metabolites in the intestinal tract and their effect on the diversity of intestinal microbial community in fish is less understood. Here, we investigated the intestinal microbial community composition of Atlantic cod after 28 days of exposure to crude oil (concentration range 0.0–0.1 mg/L). Results Analysis of PAH metabolites in bile samples confirmed that uptake and biotransformation of oil compounds occurred as a result of the exposure. Various evidence for altered microbial communities was found in fish exposed to high (0.1 mg/L) and medium (0.05 mg/L) concentrations of oil when compared to fish exposed to low oil concentration (0.01 mg/L) or no oil (control). First, altered banding patterns were observed on denaturing gradient gel electrophoresis for samples pooled from each treatment group. Secondly, based on 16S rRNA sequences, higher levels of oil exposure were associated with a loss of overall diversity of the gut microbial communities. Furthermore, 8 operational taxonomic units (OTUs) were found to have significantly different relative abundances in samples from fishes exposed to high and medium oil concentrations when compared to samples from the control group and low oil concentration. Among these, only one OTU, a Deferribacterales, had increased relative abundance in samples from fish exposed to high oil concentration. Conclusions The results presented herein contribute to a better understanding of the effects of oil contamination on the gut microbial community changes in fish and highlight the importance of further studies into the area. Our findings suggest that increased relative abundance of bacteria belonging to the order Deferribacterales may be indicative of exposure to oil at concentrations higher than 0.05 mg/L.
format Article in Journal/Newspaper
author Bagi, Andrea
Riiser, Even
Molland, Hilde
Star, Bastiaan
Haverkamp, Thomas
Sydnes, Magne
Pampanin, Daniela
author_facet Bagi, Andrea
Riiser, Even
Molland, Hilde
Star, Bastiaan
Haverkamp, Thomas
Sydnes, Magne
Pampanin, Daniela
author_sort Bagi, Andrea
title Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil
title_short Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil
title_full Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil
title_fullStr Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil
title_full_unstemmed Gastrointestinal microbial community changes in Atlantic cod (Gadus morhua) exposed to crude oil
title_sort gastrointestinal microbial community changes in atlantic cod (gadus morhua) exposed to crude oil
publisher Figshare
publishDate 2018
url https://dx.doi.org/10.6084/m9.figshare.c.4056218.v1
https://figshare.com/collections/Gastrointestinal_microbial_community_changes_in_Atlantic_cod_Gadus_morhua_exposed_to_crude_oil/4056218/1
genre atlantic cod
Gadus morhua
genre_facet atlantic cod
Gadus morhua
op_relation https://dx.doi.org/10.1186/s12866-018-1171-2
https://dx.doi.org/10.6084/m9.figshare.c.4056218
op_rights CC BY 4.0
https://creativecommons.org/licenses/by/4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.6084/m9.figshare.c.4056218.v1
https://doi.org/10.1186/s12866-018-1171-2
https://doi.org/10.6084/m9.figshare.c.4056218
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