Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco
Abstract Background Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasitesâ transmission cycles. The present study investigates the role of rodent species as potential reservoir...
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ftdatacite:10.6084/m9.figshare.c.3894694 2023-05-15T18:05:39+02:00 Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco Echchakery, Mohamed Chicharro, Carmen Boussaa, Samia Nieto, Javier Carrillo, Eugenia Ortega Sheila Moreno, Javier Boumezzough, Ali 2017 https://dx.doi.org/10.6084/m9.figshare.c.3894694 https://figshare.com/collections/Molecular_detection_of_Leishmania_infantum_and_Leishmania_tropica_in_rodent_species_from_endemic_cutaneous_leishmaniasis_areas_in_Morocco/3894694 unknown Figshare https://dx.doi.org/10.1186/s13071-017-2398-8 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Medicine Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences Sociology FOS Sociology 69999 Biological Sciences not elsewhere classified Cancer Computational Biology Collection article 2017 ftdatacite https://doi.org/10.6084/m9.figshare.c.3894694 https://doi.org/10.1186/s13071-017-2398-8 2021-11-05T12:55:41Z Abstract Background Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasitesâ transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in central Morocco, where both L. tropica and L. infantum have been reported. Methods Rodents were caught from 22 sites in central Morocco, by using Sherman metal traps, and identified morphologically. For each specimen, genomic DNA was extracted from different tissues using the Speed Tools DNA extraction Kit. Then, samples were PCR-analyzed, targeting the SSU rRNA gene to detect Leishmania spp. DNA, followed by amplification of the internal transcribed spacer 1 (ITS1) and its sequencing to identify the species. Results A total of 197 rodents belonging to ten species were captured and identified: Rattus rattus (40.61%), Mus musculus (25.38%), Apodemus sylvaticus (8.63%), Mus spretus (7.11%), Meriones shawi (5.58%), Rattus norvegicus (4.57%), Meriones libycus (3.05%), Mastomys erythroleucus (2.03%), Gerbillus campestris (2.03%) and Lemniscomys barbarus (1.01%). Molecular analysis revealed the presence of Leishmania species in 18 specimens: six R. rattus (out of 80 captured; 7.5%), 11Â M. musculus (out of 50 captured; 22%), and one R. norvegicus (out of 9 captured; 11.11%). Conclusions To the best of our knowledge, L. infantum and L. tropica were identified in rodent species for the first time in Morocco. These findings suggest that rodent species may be involved in L. infantum and L. tropica transmission cycles in this country but that further studies are needed to confirm their role as reservoirs of Leishmania species in Morocco. Article in Journal/Newspaper Rattus rattus DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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Medicine Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences Sociology FOS Sociology 69999 Biological Sciences not elsewhere classified Cancer Computational Biology |
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Medicine Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences Sociology FOS Sociology 69999 Biological Sciences not elsewhere classified Cancer Computational Biology Echchakery, Mohamed Chicharro, Carmen Boussaa, Samia Nieto, Javier Carrillo, Eugenia Ortega Sheila Moreno, Javier Boumezzough, Ali Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco |
topic_facet |
Medicine Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology FOS Biological sciences Sociology FOS Sociology 69999 Biological Sciences not elsewhere classified Cancer Computational Biology |
description |
Abstract Background Leishmaniasis remains a major public health problem in African nations, including Morocco, where little is known about the vertebrate reservoirs involved in the causal parasitesâ transmission cycles. The present study investigates the role of rodent species as potential reservoirs of Leishmania spp. in central Morocco, where both L. tropica and L. infantum have been reported. Methods Rodents were caught from 22 sites in central Morocco, by using Sherman metal traps, and identified morphologically. For each specimen, genomic DNA was extracted from different tissues using the Speed Tools DNA extraction Kit. Then, samples were PCR-analyzed, targeting the SSU rRNA gene to detect Leishmania spp. DNA, followed by amplification of the internal transcribed spacer 1 (ITS1) and its sequencing to identify the species. Results A total of 197 rodents belonging to ten species were captured and identified: Rattus rattus (40.61%), Mus musculus (25.38%), Apodemus sylvaticus (8.63%), Mus spretus (7.11%), Meriones shawi (5.58%), Rattus norvegicus (4.57%), Meriones libycus (3.05%), Mastomys erythroleucus (2.03%), Gerbillus campestris (2.03%) and Lemniscomys barbarus (1.01%). Molecular analysis revealed the presence of Leishmania species in 18 specimens: six R. rattus (out of 80 captured; 7.5%), 11Â M. musculus (out of 50 captured; 22%), and one R. norvegicus (out of 9 captured; 11.11%). Conclusions To the best of our knowledge, L. infantum and L. tropica were identified in rodent species for the first time in Morocco. These findings suggest that rodent species may be involved in L. infantum and L. tropica transmission cycles in this country but that further studies are needed to confirm their role as reservoirs of Leishmania species in Morocco. |
format |
Article in Journal/Newspaper |
author |
Echchakery, Mohamed Chicharro, Carmen Boussaa, Samia Nieto, Javier Carrillo, Eugenia Ortega Sheila Moreno, Javier Boumezzough, Ali |
author_facet |
Echchakery, Mohamed Chicharro, Carmen Boussaa, Samia Nieto, Javier Carrillo, Eugenia Ortega Sheila Moreno, Javier Boumezzough, Ali |
author_sort |
Echchakery, Mohamed |
title |
Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco |
title_short |
Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco |
title_full |
Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco |
title_fullStr |
Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco |
title_full_unstemmed |
Molecular detection of Leishmania infantum and Leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in Morocco |
title_sort |
molecular detection of leishmania infantum and leishmania tropica in rodent species from endemic cutaneous leishmaniasis areas in morocco |
publisher |
Figshare |
publishDate |
2017 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3894694 https://figshare.com/collections/Molecular_detection_of_Leishmania_infantum_and_Leishmania_tropica_in_rodent_species_from_endemic_cutaneous_leishmaniasis_areas_in_Morocco/3894694 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://dx.doi.org/10.1186/s13071-017-2398-8 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3894694 https://doi.org/10.1186/s13071-017-2398-8 |
_version_ |
1766177148834414592 |