Additional file 3: of Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss ...

• Main Authors: Hernandez, Michael, Jiang, Shan, Cole, Tracy, Chu, Shu-Hui, Fonseca, Maria, Fang, Melody, Hohsfield, Lindsay, Torres, Maria, Green, Kim, Wetsel, Rick, Mortazavi, Ali, Tenner, Andrea
• Format: Text
• Language: unknown
• Published: Figshare 2017
• Subjects: Cell Biology; Genetics; FOS Biological sciences; Neuroscience; Immunology; FOS Clinical medicine; Developmental Biology; Marine Biology; Arctic
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3882769_d3; https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_of_Prevention_of_C5aR1_signaling_delays_microglial_inflammatory_polarization_favors_clearance_pathways_and_suppresses_cognitive_loss/5418142

Aβ plaque load and CD45 expression in Arctic heterozygous for CX3CR1 and CCR2. Brain sections from Arctic, Arctic-CX3CR1+/GFP or Arctic-CCR2+/RFP reporter mice at 6 or 7 months were stained with either thioflavine (A), or an anti-Aß antibody (1536) (C) to assess the plaque load (A, C). CD45 reactivity was probed to investigate microglial activation (B,D). Arctic mice were compared to Arctic mice heterozygous for CCR2-RFP (A, B) or heterozygous for Cx3CR1-GFP (C,D). Scale bar is 100 μm. (E). Bars represent the average Field Area % of 3–4 animals per genotype (2 sections per animal). No statistically significant difference was observed in Arctic compared to Arctic-CCR2+/RFP in thioflavine (p ...