Integrated application of transcriptomics and metabolomics provides insights into glycogen content regulation in the Pacific oyster Crassostrea gigas ...

• Main Authors: Li, Busu, Song, Kai, Meng, Jie, Li, Li, Zhang, Guofan
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2017
• Subjects: Biochemistry; Genetics; FOS Biological sciences; Molecular Biology; Ecology; Biological Sciences not elsewhere classified; Inorganic Chemistry; FOS Chemical sciences; Computational Biology; Crassostrea gigas; Pacific oyster
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3877033; https://springernature.figshare.com/collections/Integrated_application_of_transcriptomics_and_metabolomics_provides_insights_into_glycogen_content_regulation_in_the_Pacific_oyster_Crassostrea_gigas/3877033

Abstract Background The Pacific oyster Crassostrea gigas is an important marine fishery resource, which contains high levels of glycogen that contributes to the flavor and the quality of the oyster. However, little is known about the molecular and chemical mechanisms underlying glycogen content differences in Pacific oysters. Using a homogeneous cultured Pacific oyster family, we explored these regulatory networks at the level of the metabolome and the transcriptome. Results Oysters with the highest and lowest natural glycogen content were selected for differential transcriptome and metabolome analysis. We identified 1888 differentially-expressed genes, seventy-five differentially-abundant metabolites, which are part of twenty-seven signaling pathways that were enriched using an integrated analysis of the interaction between the differentially-expressed genes and the differentially-abundant metabolites. Based on these results, we found that a high expression of carnitine O-palmitoyltransferase 2 (CPT2), ...