Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

• Main Authors: Eslamloo, Khalil, Xue, Xi, Hall, Jennifer, Smith, Nicole, Caballero-Solares, Albert, Parrish, Christopher, Taylor, Richard, Rise, Matthew
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Figshare 2017
• Subjects: Genetics; FOS Biological sciences; Molecular Biology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; Ecology; Immunology; FOS Clinical medicine; 69999 Biological Sciences not elsewhere classified; Inorganic Chemistry; FOS Chemical sciences; Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3875785.v1; https://figshare.com/collections/Transcriptome_profiling_of_antiviral_immune_and_dietary_fatty_acid_dependent_responses_of_Atlantic_salmon_macrophage-like_cells/3875785/1

Abstract Background Due to the limited availability and high cost of fish oil in the face of increasing aquaculture production, there is a need to reduce usage of fish oil in aquafeeds without compromising farm fish health. Therefore, the present study was conducted to determine if different levels of vegetable and fish oils can alter antiviral responses of salmon macrophage-like cells (MLCs). Atlantic salmon (Salmo salar) were fed diets containing 7.4% (FO7) or 5.1% (FO5) fish oil. These diets were designed to be relatively low in EPA + DHA (i.e. FO7: 1.41% and FO5: 1%), but near the requirement level, and resulting in comparable growth. Vegetable oil (i.e. rapeseed oil) was used to balance fish oil in experimental diets. After a 16-week feeding trial, MLCs isolated from fish in these dietary groups were stimulated by a viral mimic (dsRNA: pIC) for 6 h (qPCR assay) and 24 h (microarray and qPCR assays). Results The fatty acid composition of head kidney leukocytes varied between the two dietary groups (e.g. higher 20:5n-3 in the FO7 group). Following microarray assays using a 44K salmonid platform, Rank Products (RP) analysis showed 14 and 54 differentially expressed probes (DEP) (PFP