Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure
Abstract Background Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM10 exposure. Methods Whole genome gene expression was measured in peripheral blood of 9...
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ftdatacite:10.6084/m9.figshare.c.3857953.v1 2023-05-15T15:52:56+02:00 Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure Winckelmans, Ellen Nawrot, Tim Tsamou, Maria Hond, Elly Den Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Larebeke, Nicolas Van Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte Kok, Theo De Vrijens, Karen 2017 https://dx.doi.org/10.6084/m9.figshare.c.3857953.v1 https://figshare.com/collections/Transcriptome-wide_analyses_indicate_mitochondrial_responses_to_particulate_air_pollution_exposure/3857953/1 unknown Figshare https://dx.doi.org/10.1186/s12940-017-0292-7 https://dx.doi.org/10.6084/m9.figshare.c.3857953 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer Collection article 2017 ftdatacite https://doi.org/10.6084/m9.figshare.c.3857953.v1 https://doi.org/10.1186/s12940-017-0292-7 https://doi.org/10.6084/m9.figshare.c.3857953 2021-11-05T12:55:41Z Abstract Background Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM10 exposure. Methods Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. Article in Journal/Newspaper Carbonic acid DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer |
spellingShingle |
Biochemistry Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer Winckelmans, Ellen Nawrot, Tim Tsamou, Maria Hond, Elly Den Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Larebeke, Nicolas Van Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte Kok, Theo De Vrijens, Karen Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
topic_facet |
Biochemistry Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Cancer |
description |
Abstract Background Due to their lack of repair capacity mitochondria are critical targets for environmental toxicants. We studied genes and pathways reflecting mitochondrial responses to short- and medium-term PM10 exposure. Methods Whole genome gene expression was measured in peripheral blood of 98 adults (49% women). We performed linear regression analyses stratified by sex and adjusted for individual and temporal characteristics to investigate alterations in gene expression induced by short-term (week before blood sampling) and medium-term (month before blood sampling) PM10 exposure. Overrepresentation analyses (ConsensusPathDB) were performed to identify enriched mitochondrial associated pathways and gene ontology sets. Thirteen Human MitoCarta genes were measured by means of quantitative real-time polymerase chain reaction (qPCR) along with mitochondrial DNA (mtDNA) content in an independent validation cohort (n = 169, 55.6% women). Results Overrepresentation analyses revealed significant pathways (p-value <0.05) related to mitochondrial genome maintenance and apoptosis for short-term exposure and to the electron transport chain (ETC) for medium-term exposure in women. For men, medium-term PM10 exposure was associated with the Tri Carbonic Acid cycle. In an independent study population, we validated several ETC genes, including UQCRH and COX7C (q-value <0.05), and some genes crucial for the maintenance of the mitochondrial genome, including LONP1 (q-value: 0.07) and POLG (q-value: 0.04) in women. Conclusions In this exploratory study, we identified mitochondrial genes and pathways associated with particulate air pollution indicating upregulation of energy producing pathways as a potential mechanism to compensate for PM-induced mitochondrial damage. |
format |
Article in Journal/Newspaper |
author |
Winckelmans, Ellen Nawrot, Tim Tsamou, Maria Hond, Elly Den Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Larebeke, Nicolas Van Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte Kok, Theo De Vrijens, Karen |
author_facet |
Winckelmans, Ellen Nawrot, Tim Tsamou, Maria Hond, Elly Den Baeyens, Willy Kleinjans, Jos Lefebvre, Wouter Larebeke, Nicolas Van Peusens, Martien Plusquin, Michelle Reynders, Hans Schoeters, Greet Vanpoucke, Charlotte Kok, Theo De Vrijens, Karen |
author_sort |
Winckelmans, Ellen |
title |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_short |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_fullStr |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_full_unstemmed |
Transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
title_sort |
transcriptome-wide analyses indicate mitochondrial responses to particulate air pollution exposure |
publisher |
Figshare |
publishDate |
2017 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3857953.v1 https://figshare.com/collections/Transcriptome-wide_analyses_indicate_mitochondrial_responses_to_particulate_air_pollution_exposure/3857953/1 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_relation |
https://dx.doi.org/10.1186/s12940-017-0292-7 https://dx.doi.org/10.6084/m9.figshare.c.3857953 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3857953.v1 https://doi.org/10.1186/s12940-017-0292-7 https://doi.org/10.6084/m9.figshare.c.3857953 |
_version_ |
1766388022815752192 |