Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas
Abstract This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding fo...
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ftdatacite:10.6084/m9.figshare.c.3789493 2023-05-15T15:58:49+02:00 Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas Marie-Agnès Travers Tourbiez, Delphine Leïla Parizadeh Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie 2017 https://dx.doi.org/10.6084/m9.figshare.c.3789493 https://figshare.com/collections/Several_strains_one_disease_experimental_investigation_of_Vibrio_aestuarianus_infection_parameters_in_the_Pacific_oyster_Crassostrea_gigas/3789493 unknown Figshare https://dx.doi.org/10.1186/s13567-017-0438-1 CC BY https://creativecommons.org/licenses/by/4.0 CC-BY Microbiology FOS Biological sciences Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Infectious Diseases FOS Health sciences Computational Biology Collection article 2017 ftdatacite https://doi.org/10.6084/m9.figshare.c.3789493 https://doi.org/10.1186/s13567-017-0438-1 2021-11-05T12:55:41Z Abstract This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. Article in Journal/Newspaper Crassostrea gigas Pacific oyster DataCite Metadata Store (German National Library of Science and Technology) Pacific |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Microbiology FOS Biological sciences Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Infectious Diseases FOS Health sciences Computational Biology |
spellingShingle |
Microbiology FOS Biological sciences Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Infectious Diseases FOS Health sciences Computational Biology Marie-Agnès Travers Tourbiez, Delphine Leïla Parizadeh Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
topic_facet |
Microbiology FOS Biological sciences Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Infectious Diseases FOS Health sciences Computational Biology |
description |
Abstract This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by using seawater containing freshly shed bacteria. These results indicate that, at least with these criteria, despite V. aestuarianus strains genetic diversity, the disease process is similar. The strains isolated after the apparent re-emergence of the bacteria in 2011, do not present a more acute virulence phenotype than the reference strains isolated between 2002 and 2007. Finally, our study provides original and noteworthy data indicating that infected oysters shed bacteria at a level above the threshold of LD50 a few days before they die, meaning that infection is expected to spread in a susceptible population. |
format |
Article in Journal/Newspaper |
author |
Marie-Agnès Travers Tourbiez, Delphine Leïla Parizadeh Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie |
author_facet |
Marie-Agnès Travers Tourbiez, Delphine Leïla Parizadeh Haffner, Philippe Kozic-Djellouli, Angélique Aboubaker, Mohamed Koken, Marcel Dégremont, Lionel Lupo, Coralie |
author_sort |
Marie-Agnès Travers |
title |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_short |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas |
title_sort |
several strains, one disease: experimental investigation of vibrio aestuarianus infection parameters in the pacific oyster, crassostrea gigas |
publisher |
Figshare |
publishDate |
2017 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3789493 https://figshare.com/collections/Several_strains_one_disease_experimental_investigation_of_Vibrio_aestuarianus_infection_parameters_in_the_Pacific_oyster_Crassostrea_gigas/3789493 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://dx.doi.org/10.1186/s13567-017-0438-1 |
op_rights |
CC BY https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3789493 https://doi.org/10.1186/s13567-017-0438-1 |
_version_ |
1766394592922435584 |