Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ...

• Main Authors: Wu, Xiaoling, Yang, Cheng, Ge, Jun
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2017
• Subjects: Biochemistry; Microbiology; FOS Biological sciences; Biotechnology; Chemical Sciences not elsewhere classified; Astronomical and Space Sciences not elsewhere classified; Biological Sciences not elsewhere classified; Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3784241; https://springernature.figshare.com/collections/Green_synthesis_of_enzyme_metal-organic_framework_composites_with_high_stability_in_protein_denaturing_solvents/3784241

Abstract Objectives Enzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study. Results Encapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validated by using cytochrome c, horseradish peroxidase, and Candida antarctica lipase B as model enzymes. The stability of encapsulated enzyme was greatly increased after immobilization on MOFs. Remarkably, when exposed to protein denaturing solvents including dimethyl sulfoxide, dimethyl formamide, methanol, and ethanol, the enzyme/MOF composites still preserved almost 100% of activity. In contrast, free enzymes retained no more than 20% of their original activities at the same condition. This study shows the extraordinary protecting effect of MOF shell on increasing enzyme stability at extremely harsh conditions. Conclusion The enzyme immobilized in MOF exhibited enhanced thermal stability and high tolerance towards ...