Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ...
Abstract Objectives Enzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study. Results Encapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validate...
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ftdatacite:10.6084/m9.figshare.c.3784241.v1 2024-04-28T08:01:47+00:00 Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ... Wu, Xiaoling Yang, Cheng Ge, Jun 2017 https://dx.doi.org/10.6084/m9.figshare.c.3784241.v1 https://springernature.figshare.com/collections/Green_synthesis_of_enzyme_metal-organic_framework_composites_with_high_stability_in_protein_denaturing_solvents/3784241/1 unknown figshare https://dx.doi.org/10.6084/m9.figshare.c.3784241 Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Biochemistry Microbiology FOS Biological sciences Biotechnology Chemical Sciences not elsewhere classified Astronomical and Space Sciences not elsewhere classified Biological Sciences not elsewhere classified Collection article 2017 ftdatacite https://doi.org/10.6084/m9.figshare.c.3784241.v110.6084/m9.figshare.c.3784241 2024-04-02T11:54:48Z Abstract Objectives Enzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study. Results Encapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validated by using cytochrome c, horseradish peroxidase, and Candida antarctica lipase B as model enzymes. The stability of encapsulated enzyme was greatly increased after immobilization on MOFs. Remarkably, when exposed to protein denaturing solvents including dimethyl sulfoxide, dimethyl formamide, methanol, and ethanol, the enzyme/MOF composites still preserved almost 100% of activity. In contrast, free enzymes retained no more than 20% of their original activities at the same condition. This study shows the extraordinary protecting effect of MOF shell on increasing enzyme stability at extremely harsh conditions. Conclusion The enzyme immobilized in MOF exhibited enhanced thermal stability and high tolerance towards ... Article in Journal/Newspaper Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
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Biochemistry Microbiology FOS Biological sciences Biotechnology Chemical Sciences not elsewhere classified Astronomical and Space Sciences not elsewhere classified Biological Sciences not elsewhere classified |
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Biochemistry Microbiology FOS Biological sciences Biotechnology Chemical Sciences not elsewhere classified Astronomical and Space Sciences not elsewhere classified Biological Sciences not elsewhere classified Wu, Xiaoling Yang, Cheng Ge, Jun Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ... |
topic_facet |
Biochemistry Microbiology FOS Biological sciences Biotechnology Chemical Sciences not elsewhere classified Astronomical and Space Sciences not elsewhere classified Biological Sciences not elsewhere classified |
description |
Abstract Objectives Enzyme/metal-organic framework composites with high stability in protein denaturing solvents were reported in this study. Results Encapsulation of enzyme in metal-organic frameworks (MOFs) via co-precipitation process was realized, and the generality of the synthesis was validated by using cytochrome c, horseradish peroxidase, and Candida antarctica lipase B as model enzymes. The stability of encapsulated enzyme was greatly increased after immobilization on MOFs. Remarkably, when exposed to protein denaturing solvents including dimethyl sulfoxide, dimethyl formamide, methanol, and ethanol, the enzyme/MOF composites still preserved almost 100% of activity. In contrast, free enzymes retained no more than 20% of their original activities at the same condition. This study shows the extraordinary protecting effect of MOF shell on increasing enzyme stability at extremely harsh conditions. Conclusion The enzyme immobilized in MOF exhibited enhanced thermal stability and high tolerance towards ... |
format |
Article in Journal/Newspaper |
author |
Wu, Xiaoling Yang, Cheng Ge, Jun |
author_facet |
Wu, Xiaoling Yang, Cheng Ge, Jun |
author_sort |
Wu, Xiaoling |
title |
Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ... |
title_short |
Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ... |
title_full |
Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ... |
title_fullStr |
Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ... |
title_full_unstemmed |
Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ... |
title_sort |
green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents ... |
publisher |
figshare |
publishDate |
2017 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3784241.v1 https://springernature.figshare.com/collections/Green_synthesis_of_enzyme_metal-organic_framework_composites_with_high_stability_in_protein_denaturing_solvents/3784241/1 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://dx.doi.org/10.6084/m9.figshare.c.3784241 |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3784241.v110.6084/m9.figshare.c.3784241 |
_version_ |
1797573374204968960 |