Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa
Abstract Background Winter-ulcer Moritella viscosa infections continue to be a significant burden in Atlantic salmon (Salmo salar L.) farming. M. viscosa comprises two main clusters that differ in genetic variation and phenotypes including virulence. Horizontal gene transfer through acquisition and...
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ftdatacite:10.6084/m9.figshare.c.3747611.v1 2023-05-15T15:32:41+02:00 Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa Karlsen, Christian Hjerde, Erik Klemetsen, Terje Willassen, Nils 2017 https://dx.doi.org/10.6084/m9.figshare.c.3747611.v1 https://figshare.com/collections/Pan_genome_and_CRISPR_analyses_of_the_bacterial_fish_pathogen_Moritella_viscosa/3747611/1 unknown Figshare https://dx.doi.org/10.1186/s12864-017-3693-7 https://dx.doi.org/10.6084/m9.figshare.c.3747611 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Medicine Microbiology FOS Biological sciences Genetics Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 60506 Virology Computational Biology Collection article 2017 ftdatacite https://doi.org/10.6084/m9.figshare.c.3747611.v1 https://doi.org/10.1186/s12864-017-3693-7 https://doi.org/10.6084/m9.figshare.c.3747611 2021-11-05T12:55:41Z Abstract Background Winter-ulcer Moritella viscosa infections continue to be a significant burden in Atlantic salmon (Salmo salar L.) farming. M. viscosa comprises two main clusters that differ in genetic variation and phenotypes including virulence. Horizontal gene transfer through acquisition and loss of mobile genetic elements (MGEs) is a major driving force of bacterial diversification. To gain insight into genomic traits that could affect sublineage evolution within this bacterium we examined the genome sequences of twelve M. viscosa strains. Matches between M. viscosa clustered, regularly interspaced, short palindromic, repeats and associated cas genes (CRISPR-Cas) were analysed to correlate CRISPR-Cas with adaptive immunity against MGEs. Results The comparative genomic analysis of M. viscosa isolates from across the North Atlantic region and from different fish species support delineation of M. viscosa into four phylogenetic lineages. The results showed that M. viscosa carries two distinct variants of the CRISPR-Cas subtype I-F systems and that CRISPR features follow the phylogenetic lineages. A subset of the spacer content match prophage and plasmid genes dispersed among the M. viscosa strains. Further analysis revealed that prophage and plasmid-like element distribution were reflected in the content of the CRISPR-spacer profiles. Conclusions Our data suggests that CRISPR-Cas mediated interactions with MGEs impact genome properties among M. viscosa, and that patterns in spacer and MGE distributions are linked to strain relationships. Article in Journal/Newspaper Atlantic salmon North Atlantic Salmo salar DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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language |
unknown |
topic |
Medicine Microbiology FOS Biological sciences Genetics Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 60506 Virology Computational Biology |
spellingShingle |
Medicine Microbiology FOS Biological sciences Genetics Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 60506 Virology Computational Biology Karlsen, Christian Hjerde, Erik Klemetsen, Terje Willassen, Nils Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
topic_facet |
Medicine Microbiology FOS Biological sciences Genetics Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 60506 Virology Computational Biology |
description |
Abstract Background Winter-ulcer Moritella viscosa infections continue to be a significant burden in Atlantic salmon (Salmo salar L.) farming. M. viscosa comprises two main clusters that differ in genetic variation and phenotypes including virulence. Horizontal gene transfer through acquisition and loss of mobile genetic elements (MGEs) is a major driving force of bacterial diversification. To gain insight into genomic traits that could affect sublineage evolution within this bacterium we examined the genome sequences of twelve M. viscosa strains. Matches between M. viscosa clustered, regularly interspaced, short palindromic, repeats and associated cas genes (CRISPR-Cas) were analysed to correlate CRISPR-Cas with adaptive immunity against MGEs. Results The comparative genomic analysis of M. viscosa isolates from across the North Atlantic region and from different fish species support delineation of M. viscosa into four phylogenetic lineages. The results showed that M. viscosa carries two distinct variants of the CRISPR-Cas subtype I-F systems and that CRISPR features follow the phylogenetic lineages. A subset of the spacer content match prophage and plasmid genes dispersed among the M. viscosa strains. Further analysis revealed that prophage and plasmid-like element distribution were reflected in the content of the CRISPR-spacer profiles. Conclusions Our data suggests that CRISPR-Cas mediated interactions with MGEs impact genome properties among M. viscosa, and that patterns in spacer and MGE distributions are linked to strain relationships. |
format |
Article in Journal/Newspaper |
author |
Karlsen, Christian Hjerde, Erik Klemetsen, Terje Willassen, Nils |
author_facet |
Karlsen, Christian Hjerde, Erik Klemetsen, Terje Willassen, Nils |
author_sort |
Karlsen, Christian |
title |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_short |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_full |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_fullStr |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_full_unstemmed |
Pan genome and CRISPR analyses of the bacterial fish pathogen Moritella viscosa |
title_sort |
pan genome and crispr analyses of the bacterial fish pathogen moritella viscosa |
publisher |
Figshare |
publishDate |
2017 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3747611.v1 https://figshare.com/collections/Pan_genome_and_CRISPR_analyses_of_the_bacterial_fish_pathogen_Moritella_viscosa/3747611/1 |
genre |
Atlantic salmon North Atlantic Salmo salar |
genre_facet |
Atlantic salmon North Atlantic Salmo salar |
op_relation |
https://dx.doi.org/10.1186/s12864-017-3693-7 https://dx.doi.org/10.6084/m9.figshare.c.3747611 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3747611.v1 https://doi.org/10.1186/s12864-017-3693-7 https://doi.org/10.6084/m9.figshare.c.3747611 |
_version_ |
1766363176492859392 |