Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers
Abstract Background Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are...
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ftdatacite:10.6084/m9.figshare.c.3735463 2023-05-15T18:34:48+02:00 Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers Anne-Merethe Hanssen Kindlund, Bert Stenklev, Niels Anne-Sofie Furberg Fismen, Silje Olsen, Renate Johannessen, Mona Sollid, Johanna 2017 https://dx.doi.org/10.6084/m9.figshare.c.3735463 https://figshare.com/collections/Localization_of_Staphylococcus_aureus_in_tissue_from_the_nasal_vestibule_in_healthy_carriers/3735463 unknown Figshare https://dx.doi.org/10.1186/s12866-017-0997-3 CC BY https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine Cancer Infectious Diseases FOS Health sciences Virology Collection article 2017 ftdatacite https://doi.org/10.6084/m9.figshare.c.3735463 https://doi.org/10.1186/s12866-017-0997-3 2021-11-05T12:55:41Z Abstract Background Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are found in vestibulum nasi. Our aim was to describe the localization of S. aureus in nasal tissue from healthy carriers. Methods Punch skin biopsies were taken from vestibulum nasi from healthy volunteers (S. aureus carriers and non−/intermittent carriers, n = 39) attending the population-based Tromsø 6 study. The tissue samples were processed as frozen sections before immunostaining with a specific S. aureus antibody, and finally evaluated by a confocal laser-scanning microscope. Results Our results suggest that S. aureus colonize both the upper and lower layers of the epidermis within the nasal epithelium of healthy individuals. The number of S. aureus in epidermis was surprisingly low. Intracellular localization of S. aureus in nasal tissue from healthy individuals was also detected. Conclusions Knowledge of the exact localization of S. aureus in nasal tissue is important for the understanding of the host responses against S. aureus. Our results may have consequences for the eradication strategy of S. aureus in carriers, and further work can provide us with tools for targeted prevention of S. aureus colonisation and infection. Article in Journal/Newspaper Tromsø DataCite Metadata Store (German National Library of Science and Technology) Nares ENVELOPE(158.167,158.167,-81.450,-81.450) Nasi ENVELOPE(-23.036,-23.036,66.336,66.336) Tromsø |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
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unknown |
topic |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine Cancer Infectious Diseases FOS Health sciences Virology |
spellingShingle |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine Cancer Infectious Diseases FOS Health sciences Virology Anne-Merethe Hanssen Kindlund, Bert Stenklev, Niels Anne-Sofie Furberg Fismen, Silje Olsen, Renate Johannessen, Mona Sollid, Johanna Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
topic_facet |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences Ecology Immunology FOS Clinical medicine Cancer Infectious Diseases FOS Health sciences Virology |
description |
Abstract Background Colonization of the body is an important step in Staphylococcus aureus infection. S. aureus colonizes skin and mucous membranes in humans and several animal species. One important ecological niche of S. aureus is the anterior nares. More than 60% of the S. aureus in the nose are found in vestibulum nasi. Our aim was to describe the localization of S. aureus in nasal tissue from healthy carriers. Methods Punch skin biopsies were taken from vestibulum nasi from healthy volunteers (S. aureus carriers and non−/intermittent carriers, n = 39) attending the population-based Tromsø 6 study. The tissue samples were processed as frozen sections before immunostaining with a specific S. aureus antibody, and finally evaluated by a confocal laser-scanning microscope. Results Our results suggest that S. aureus colonize both the upper and lower layers of the epidermis within the nasal epithelium of healthy individuals. The number of S. aureus in epidermis was surprisingly low. Intracellular localization of S. aureus in nasal tissue from healthy individuals was also detected. Conclusions Knowledge of the exact localization of S. aureus in nasal tissue is important for the understanding of the host responses against S. aureus. Our results may have consequences for the eradication strategy of S. aureus in carriers, and further work can provide us with tools for targeted prevention of S. aureus colonisation and infection. |
format |
Article in Journal/Newspaper |
author |
Anne-Merethe Hanssen Kindlund, Bert Stenklev, Niels Anne-Sofie Furberg Fismen, Silje Olsen, Renate Johannessen, Mona Sollid, Johanna |
author_facet |
Anne-Merethe Hanssen Kindlund, Bert Stenklev, Niels Anne-Sofie Furberg Fismen, Silje Olsen, Renate Johannessen, Mona Sollid, Johanna |
author_sort |
Anne-Merethe Hanssen |
title |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_short |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_full |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_fullStr |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_full_unstemmed |
Localization of Staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
title_sort |
localization of staphylococcus aureus in tissue from the nasal vestibule in healthy carriers |
publisher |
Figshare |
publishDate |
2017 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3735463 https://figshare.com/collections/Localization_of_Staphylococcus_aureus_in_tissue_from_the_nasal_vestibule_in_healthy_carriers/3735463 |
long_lat |
ENVELOPE(158.167,158.167,-81.450,-81.450) ENVELOPE(-23.036,-23.036,66.336,66.336) |
geographic |
Nares Nasi Tromsø |
geographic_facet |
Nares Nasi Tromsø |
genre |
Tromsø |
genre_facet |
Tromsø |
op_relation |
https://dx.doi.org/10.1186/s12866-017-0997-3 |
op_rights |
CC BY https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3735463 https://doi.org/10.1186/s12866-017-0997-3 |
_version_ |
1766219718512869376 |