Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism
Abstract Background Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liv...
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ftdatacite:10.6084/m9.figshare.c.3645989.v1 2023-05-15T15:27:23+02:00 Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism Fekadu Yadetie Bjørneklett, Silje Garberg, Hilde Oveland, Eystein Berven, Frode Goksøyr, Anders Karlsen, Odd 2016 https://dx.doi.org/10.6084/m9.figshare.c.3645989.v1 https://figshare.com/collections/Quantitative_analyses_of_the_hepatic_proteome_of_methylmercury-exposed_Atlantic_cod_Gadus_morhua_suggest_oxidative_stress-mediated_effects_on_cellular_energy_metabolism/3645989/1 unknown Figshare https://dx.doi.org/10.1186/s12864-016-2864-2 https://dx.doi.org/10.6084/m9.figshare.c.3645989 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Genetics FOS Biological sciences Molecular Biology Physiology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Computational Biology Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3645989.v1 https://doi.org/10.1186/s12864-016-2864-2 https://doi.org/10.6084/m9.figshare.c.3645989 2021-11-05T12:55:41Z Abstract Background Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver proteome of Atlantic cod (Gadus morhua) exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. Results Out of a toltal of 1143 proteins quantified, 125 proteins were differentially regulated between MeHg-treated samples and controls. Using various bioinformatics tools, we performed gene ontology, pathway and network enrichment analysis, which indicated that proteins and pathways mainly related to energy metabolism, antioxidant defense, cytoskeleton remodeling, and protein synthesis were regulated in the hepatic proteome after MeHg exposure. Comparison with previous gene expression data strengthened these results, and further supported that MeHg predominantly affects many energy metabolism pathways, presumably through its strong induction of oxidative stress. Some enzymes known to have functionally important oxidation-sensitive cysteine residues in other animals are among the differentially regulated proteins, suggesting their modulations by MeHg-induced oxidative stress. Integrated analysis of the proteomics dataset combined with previous gene expression dataset showed a more pronounced effect of MeHg on amino acid, glucose and fatty acid metabolic pathways, and suggested possible interactions of the cellular energy metabolism and antioxidant defense pathways. Conclusions MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver. The energy pathways appear to be modulated through MeHg-induced oxidative stress, possibly mediated by oxidation sensitive enzymes. Article in Journal/Newspaper atlantic cod Gadus morhua DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Genetics FOS Biological sciences Molecular Biology Physiology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Computational Biology |
spellingShingle |
Biochemistry Genetics FOS Biological sciences Molecular Biology Physiology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Computational Biology Fekadu Yadetie Bjørneklett, Silje Garberg, Hilde Oveland, Eystein Berven, Frode Goksøyr, Anders Karlsen, Odd Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
topic_facet |
Biochemistry Genetics FOS Biological sciences Molecular Biology Physiology 39999 Chemical Sciences not elsewhere classified FOS Chemical sciences 69999 Biological Sciences not elsewhere classified Inorganic Chemistry Computational Biology |
description |
Abstract Background Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver proteome of Atlantic cod (Gadus morhua) exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. Results Out of a toltal of 1143 proteins quantified, 125 proteins were differentially regulated between MeHg-treated samples and controls. Using various bioinformatics tools, we performed gene ontology, pathway and network enrichment analysis, which indicated that proteins and pathways mainly related to energy metabolism, antioxidant defense, cytoskeleton remodeling, and protein synthesis were regulated in the hepatic proteome after MeHg exposure. Comparison with previous gene expression data strengthened these results, and further supported that MeHg predominantly affects many energy metabolism pathways, presumably through its strong induction of oxidative stress. Some enzymes known to have functionally important oxidation-sensitive cysteine residues in other animals are among the differentially regulated proteins, suggesting their modulations by MeHg-induced oxidative stress. Integrated analysis of the proteomics dataset combined with previous gene expression dataset showed a more pronounced effect of MeHg on amino acid, glucose and fatty acid metabolic pathways, and suggested possible interactions of the cellular energy metabolism and antioxidant defense pathways. Conclusions MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver. The energy pathways appear to be modulated through MeHg-induced oxidative stress, possibly mediated by oxidation sensitive enzymes. |
format |
Article in Journal/Newspaper |
author |
Fekadu Yadetie Bjørneklett, Silje Garberg, Hilde Oveland, Eystein Berven, Frode Goksøyr, Anders Karlsen, Odd |
author_facet |
Fekadu Yadetie Bjørneklett, Silje Garberg, Hilde Oveland, Eystein Berven, Frode Goksøyr, Anders Karlsen, Odd |
author_sort |
Fekadu Yadetie |
title |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_short |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_full |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_fullStr |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_full_unstemmed |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_sort |
quantitative analyses of the hepatic proteome of methylmercury-exposed atlantic cod (gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3645989.v1 https://figshare.com/collections/Quantitative_analyses_of_the_hepatic_proteome_of_methylmercury-exposed_Atlantic_cod_Gadus_morhua_suggest_oxidative_stress-mediated_effects_on_cellular_energy_metabolism/3645989/1 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
https://dx.doi.org/10.1186/s12864-016-2864-2 https://dx.doi.org/10.6084/m9.figshare.c.3645989 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3645989.v1 https://doi.org/10.1186/s12864-016-2864-2 https://doi.org/10.6084/m9.figshare.c.3645989 |
_version_ |
1766357822570758144 |