Infectious salmon anaemia virus (ISAV) mucosal infection in Atlantic salmon

• Main Authors: Aamelfot, Maria, McBeath, Alastair, Debes Christiansen, Matejusova, Iveta, Falk, Knut
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Figshare 2015
• Subjects: Medicine; Microbiology; FOS Biological sciences; Biotechnology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; Ecology; Immunology; FOS Clinical medicine; 69999 Biological Sciences not elsewhere classified; 110309 Infectious Diseases; FOS Health sciences; 60506 Virology; Computational Biology; Norway; Atlantic salmon; Salmo salar
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3644606; https://figshare.com/collections/Infectious_salmon_anaemia_virus_ISAV_mucosal_infection_in_Atlantic_salmon/3644606

Abstract All viruses infecting fish must cross the surface mucosal barrier to successfully enter a host. Infectious salmon anaemia virus (ISAV), the causative agent of the economically important infectious salmon anaemia (ISA) in Atlantic salmon, Salmo salar L., has been shown to use the gills as its entry point. However, other entry ports have not been investigated despite the expression of virus receptors on the surface of epithelial cells in the skin, the gastrointestinal (GI) tract and the conjunctiva. Here we investigate the ISAV mucosal infection in Atlantic salmon after experimental immersion (bath) challenge and in farmed fish collected from a confirmed outbreak of ISA in Norway. We show for the first time evidence of early replication in several mucosal surfaces in addition to the gills, including the pectoral fin, skin and GI tract suggesting several potential entry points for the virus. Initially, the infection is localized and primarily infecting epithelial cells, however at later stages it becomes systemic, infecting the endothelial cells lining the circulatory system. Viruses of low and high virulence used in the challenge revealed possible variation in virus progression during infection at the mucosal surfaces.