Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections
Abstract Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genu...
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ftdatacite:10.6084/m9.figshare.c.3638876 2023-05-15T15:58:18+02:00 Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections Green, Timothy Vergnes, Agnes Montagnani, Caroline Lorgeril, Julien 2016 https://dx.doi.org/10.6084/m9.figshare.c.3638876 https://figshare.com/collections/Distinct_immune_responses_of_juvenile_and_adult_oysters_Crassostrea_gigas_to_viral_and_bacterial_infections/3638876 unknown Figshare https://dx.doi.org/10.1186/s13567-016-0356-7 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Medicine Microbiology FOS Biological sciences Genetics Physiology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Science Policy Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Cancer 111714 Mental Health FOS Health sciences 110309 Infectious Diseases Computational Biology Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3638876 https://doi.org/10.1186/s13567-016-0356-7 2021-11-05T12:55:41Z Abstract Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oysterâ s microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host. Article in Journal/Newspaper Crassostrea gigas DataCite Metadata Store (German National Library of Science and Technology) Pacific |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Medicine Microbiology FOS Biological sciences Genetics Physiology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Science Policy Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Cancer 111714 Mental Health FOS Health sciences 110309 Infectious Diseases Computational Biology |
spellingShingle |
Medicine Microbiology FOS Biological sciences Genetics Physiology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Science Policy Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Cancer 111714 Mental Health FOS Health sciences 110309 Infectious Diseases Computational Biology Green, Timothy Vergnes, Agnes Montagnani, Caroline Lorgeril, Julien Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
topic_facet |
Medicine Microbiology FOS Biological sciences Genetics Physiology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Science Policy Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Cancer 111714 Mental Health FOS Health sciences 110309 Infectious Diseases Computational Biology |
description |
Abstract Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oysterâ s microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host. |
format |
Article in Journal/Newspaper |
author |
Green, Timothy Vergnes, Agnes Montagnani, Caroline Lorgeril, Julien |
author_facet |
Green, Timothy Vergnes, Agnes Montagnani, Caroline Lorgeril, Julien |
author_sort |
Green, Timothy |
title |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_short |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_full |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_fullStr |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_full_unstemmed |
Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections |
title_sort |
distinct immune responses of juvenile and adult oysters (crassostrea gigas) to viral and bacterial infections |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3638876 https://figshare.com/collections/Distinct_immune_responses_of_juvenile_and_adult_oysters_Crassostrea_gigas_to_viral_and_bacterial_infections/3638876 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
https://dx.doi.org/10.1186/s13567-016-0356-7 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3638876 https://doi.org/10.1186/s13567-016-0356-7 |
_version_ |
1766394032233119744 |