When the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain
Abstract Background During long dives, the brain of whales and seals experiences a reduced supply of oxygen (hypoxia). The brain neurons of the hooded seal (Cystophora cristata) are more tolerant towards low-oxygen conditions than those of mice, and also better survive other hypoxia-related stress c...
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ftdatacite:10.6084/m9.figshare.c.3636611.v1 2023-05-15T15:59:54+02:00 When the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain Fabrizius, Andrej Hoff, Mariana Engler, Gerhard Folkow, Lars Burmester, Thorsten 2016 https://dx.doi.org/10.6084/m9.figshare.c.3636611.v1 https://figshare.com/collections/When_the_brain_goes_diving_transcriptome_analysis_reveals_a_reduced_aerobic_energy_metabolism_and_increased_stress_proteins_in_the_seal_brain/3636611/1 unknown Figshare https://dx.doi.org/10.1186/s12864-016-2892-y https://dx.doi.org/10.6084/m9.figshare.c.3636611 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Cell Biology Molecular Biology Neuroscience Physiology FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 111714 Mental Health FOS Health sciences Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3636611.v1 https://doi.org/10.1186/s12864-016-2892-y https://doi.org/10.6084/m9.figshare.c.3636611 2021-11-05T12:55:41Z Abstract Background During long dives, the brain of whales and seals experiences a reduced supply of oxygen (hypoxia). The brain neurons of the hooded seal (Cystophora cristata) are more tolerant towards low-oxygen conditions than those of mice, and also better survive other hypoxia-related stress conditions like a reduction in glucose supply and high concentrations of lactate. Little is known about the molecular mechanisms that support the hypoxia tolerance of the diving brain. Results Here we employed RNA-seq to approach the molecular basis of the unusual stress tolerance of the seal brain. An Illumina-generated transcriptome of the visual cortex of the hooded seal was compared with that of the ferret (Mustela putorius furo), which served as a terrestrial relative. Gene ontology analyses showed a significant enrichment of transcripts related to translation and aerobic energy production in the ferret but not in the seal brain. Clusterin, an extracellular chaperone, is the most highly expressed gene in the seal brain and fourfold higher than in the ferret or any other mammalian brain transcriptome. The largest difference was found for S100B, a calcium-binding stress protein with pleiotropic function, which was 38-fold enriched in the seal brain. Notably, significant enrichment of S100B mRNA was also found in the transcriptomes of whale brains, but not in the brains of terrestrial mammals. Conclusion Comparative transcriptomics indicates a lower aerobic capacity of the seal brain, which may be interpreted as a general energy saving strategy. Elevated expression of stress-related genes, such as clusterin and S100B, possibly contributes to the remarkable hypoxia tolerance of the brain of the hooded seal. Moreover, high levels of S100B that possibly protect the brain appear to be the result of the convergent adaptation of diving mammals. Article in Journal/Newspaper Cystophora cristata hooded seal DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Cell Biology Molecular Biology Neuroscience Physiology FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 111714 Mental Health FOS Health sciences |
spellingShingle |
Cell Biology Molecular Biology Neuroscience Physiology FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 111714 Mental Health FOS Health sciences Fabrizius, Andrej Hoff, Mariana Engler, Gerhard Folkow, Lars Burmester, Thorsten When the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain |
topic_facet |
Cell Biology Molecular Biology Neuroscience Physiology FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 111714 Mental Health FOS Health sciences |
description |
Abstract Background During long dives, the brain of whales and seals experiences a reduced supply of oxygen (hypoxia). The brain neurons of the hooded seal (Cystophora cristata) are more tolerant towards low-oxygen conditions than those of mice, and also better survive other hypoxia-related stress conditions like a reduction in glucose supply and high concentrations of lactate. Little is known about the molecular mechanisms that support the hypoxia tolerance of the diving brain. Results Here we employed RNA-seq to approach the molecular basis of the unusual stress tolerance of the seal brain. An Illumina-generated transcriptome of the visual cortex of the hooded seal was compared with that of the ferret (Mustela putorius furo), which served as a terrestrial relative. Gene ontology analyses showed a significant enrichment of transcripts related to translation and aerobic energy production in the ferret but not in the seal brain. Clusterin, an extracellular chaperone, is the most highly expressed gene in the seal brain and fourfold higher than in the ferret or any other mammalian brain transcriptome. The largest difference was found for S100B, a calcium-binding stress protein with pleiotropic function, which was 38-fold enriched in the seal brain. Notably, significant enrichment of S100B mRNA was also found in the transcriptomes of whale brains, but not in the brains of terrestrial mammals. Conclusion Comparative transcriptomics indicates a lower aerobic capacity of the seal brain, which may be interpreted as a general energy saving strategy. Elevated expression of stress-related genes, such as clusterin and S100B, possibly contributes to the remarkable hypoxia tolerance of the brain of the hooded seal. Moreover, high levels of S100B that possibly protect the brain appear to be the result of the convergent adaptation of diving mammals. |
format |
Article in Journal/Newspaper |
author |
Fabrizius, Andrej Hoff, Mariana Engler, Gerhard Folkow, Lars Burmester, Thorsten |
author_facet |
Fabrizius, Andrej Hoff, Mariana Engler, Gerhard Folkow, Lars Burmester, Thorsten |
author_sort |
Fabrizius, Andrej |
title |
When the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain |
title_short |
When the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain |
title_full |
When the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain |
title_fullStr |
When the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain |
title_full_unstemmed |
When the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain |
title_sort |
when the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3636611.v1 https://figshare.com/collections/When_the_brain_goes_diving_transcriptome_analysis_reveals_a_reduced_aerobic_energy_metabolism_and_increased_stress_proteins_in_the_seal_brain/3636611/1 |
genre |
Cystophora cristata hooded seal |
genre_facet |
Cystophora cristata hooded seal |
op_relation |
https://dx.doi.org/10.1186/s12864-016-2892-y https://dx.doi.org/10.6084/m9.figshare.c.3636611 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3636611.v1 https://doi.org/10.1186/s12864-016-2892-y https://doi.org/10.6084/m9.figshare.c.3636611 |
_version_ |
1766395795727187968 |