De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica
Abstract Background FapR protein from the psychrotrophic species Exiguobacterium antarcticum B7 was expressed and purified, and subsequently evaluated for its capacity to bind to the promoter regions of the fabH1-fabF and fapR-plsX-fabD-fabG operons, using electrophoretic mobility shift assay. The g...
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ftdatacite:10.6084/m9.figshare.c.3633086.v1 2023-05-15T14:02:57+02:00 De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica Baraúna, Rafael Graças, Diego Das Nunes, Catarina Schneider, Maria Silva, Artur Carepo, Marta 2016 https://dx.doi.org/10.6084/m9.figshare.c.3633086.v1 https://figshare.com/collections/De_novo_synthesis_of_fatty_acids_is_regulated_by_FapR_protein_in_Exiguobacterium_antarcticum_B7_a_psychrotrophic_bacterium_isolated_from_Antarctica/3633086/1 unknown Figshare https://dx.doi.org/10.1186/s13104-016-2250-9 https://dx.doi.org/10.6084/m9.figshare.c.3633086 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biophysics Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology Evolutionary Biology Immunology FOS Clinical medicine Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Computational Biology Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3633086.v1 https://doi.org/10.1186/s13104-016-2250-9 https://doi.org/10.6084/m9.figshare.c.3633086 2021-11-05T12:55:41Z Abstract Background FapR protein from the psychrotrophic species Exiguobacterium antarcticum B7 was expressed and purified, and subsequently evaluated for its capacity to bind to the promoter regions of the fabH1-fabF and fapR-plsX-fabD-fabG operons, using electrophoretic mobility shift assay. The genes that compose these operons encode for enzymes involved in the de novo synthesis of fatty acids molecules. In Bacillus subtilis, FapR regulates the expression of these operons, and consequently has influence in the synthesis of long or short-chain fatty acids. To analyze the bacterial cold adaptation, this is an important metabolic pathway because psychrotrophic microrganisms tend to synthesize short and branched-chain unsaturated fatty acids at cold to maintain cell membrane fluidity. Results In this work, it was observed that recombinant protein was able to bind to the promoter of the fully amplified fabH1-fabF and fapR-plsX-fabD-fabG operons. However, FapR was unable to bind to the promoter of fapR-plsX-fabD-fabG operon when synthesized only up to the protein-binding palindrome 5′-TTAGTACCAGATACTAA-3′, thus showing the importance of the entire promoter sequence for the correct protein-DNA interaction. Conclusions Through this observation, we demonstrate that the FapR protein possibly regulates the same operons as described for other species, which emphasizes its importance to cold adaptation process of E. antarcticum B7, a psychrotrophic bacterium isolated at Antarctica. Article in Journal/Newspaper Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biophysics Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology Evolutionary Biology Immunology FOS Clinical medicine Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Computational Biology |
spellingShingle |
Biophysics Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology Evolutionary Biology Immunology FOS Clinical medicine Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Computational Biology Baraúna, Rafael Graças, Diego Das Nunes, Catarina Schneider, Maria Silva, Artur Carepo, Marta De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica |
topic_facet |
Biophysics Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Genetics Molecular Biology Evolutionary Biology Immunology FOS Clinical medicine Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Computational Biology |
description |
Abstract Background FapR protein from the psychrotrophic species Exiguobacterium antarcticum B7 was expressed and purified, and subsequently evaluated for its capacity to bind to the promoter regions of the fabH1-fabF and fapR-plsX-fabD-fabG operons, using electrophoretic mobility shift assay. The genes that compose these operons encode for enzymes involved in the de novo synthesis of fatty acids molecules. In Bacillus subtilis, FapR regulates the expression of these operons, and consequently has influence in the synthesis of long or short-chain fatty acids. To analyze the bacterial cold adaptation, this is an important metabolic pathway because psychrotrophic microrganisms tend to synthesize short and branched-chain unsaturated fatty acids at cold to maintain cell membrane fluidity. Results In this work, it was observed that recombinant protein was able to bind to the promoter of the fully amplified fabH1-fabF and fapR-plsX-fabD-fabG operons. However, FapR was unable to bind to the promoter of fapR-plsX-fabD-fabG operon when synthesized only up to the protein-binding palindrome 5′-TTAGTACCAGATACTAA-3′, thus showing the importance of the entire promoter sequence for the correct protein-DNA interaction. Conclusions Through this observation, we demonstrate that the FapR protein possibly regulates the same operons as described for other species, which emphasizes its importance to cold adaptation process of E. antarcticum B7, a psychrotrophic bacterium isolated at Antarctica. |
format |
Article in Journal/Newspaper |
author |
Baraúna, Rafael Graças, Diego Das Nunes, Catarina Schneider, Maria Silva, Artur Carepo, Marta |
author_facet |
Baraúna, Rafael Graças, Diego Das Nunes, Catarina Schneider, Maria Silva, Artur Carepo, Marta |
author_sort |
Baraúna, Rafael |
title |
De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica |
title_short |
De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica |
title_full |
De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica |
title_fullStr |
De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica |
title_full_unstemmed |
De novo synthesis of fatty acids is regulated by FapR protein in Exiguobacterium antarcticum B7, a psychrotrophic bacterium isolated from Antarctica |
title_sort |
de novo synthesis of fatty acids is regulated by fapr protein in exiguobacterium antarcticum b7, a psychrotrophic bacterium isolated from antarctica |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3633086.v1 https://figshare.com/collections/De_novo_synthesis_of_fatty_acids_is_regulated_by_FapR_protein_in_Exiguobacterium_antarcticum_B7_a_psychrotrophic_bacterium_isolated_from_Antarctica/3633086/1 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://dx.doi.org/10.1186/s13104-016-2250-9 https://dx.doi.org/10.6084/m9.figshare.c.3633086 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3633086.v1 https://doi.org/10.1186/s13104-016-2250-9 https://doi.org/10.6084/m9.figshare.c.3633086 |
_version_ |
1766273398434955264 |