Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids
Abstract Background The study presents the phenotypic and genetic characterization of selected P. salmonis isolates from Atlantic salmon and rainbow trout suffering from SRS (salmonid rickettsial septicemia) in Chile and in Canada. The phenotypic characterization of the P. salmonis isolates were bas...
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ftdatacite:10.6084/m9.figshare.c.3628286 2023-05-15T15:32:36+02:00 Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids Otterlei, Alexander ĂYvind Brevik Jensen, Daniel Duesund, Henrik Sommerset, Ingunn Frost, Petter Mendoza, Julio McKenzie, Peter Nylund, Are Apablaza, Patricia 2016 https://dx.doi.org/10.6084/m9.figshare.c.3628286 https://figshare.com/collections/Phenotypic_and_genetic_characterization_of_Piscirickettsia_salmonis_from_Chilean_and_Canadian_salmonids/3628286 unknown Figshare https://dx.doi.org/10.1186/s12917-016-0681-0 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Medicine Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3628286 https://doi.org/10.1186/s12917-016-0681-0 2021-11-05T12:55:41Z Abstract Background The study presents the phenotypic and genetic characterization of selected P. salmonis isolates from Atlantic salmon and rainbow trout suffering from SRS (salmonid rickettsial septicemia) in Chile and in Canada. The phenotypic characterization of the P. salmonis isolates were based on growth on different agar media (including a newly developed medium), different growth temperatures, antibiotics susceptibility and biochemical tests. Results This is the first study differentiating Chilean P. salmonis isolates into two separate genetic groups. Genotyping, based on 16S rRNA-ITS and concatenated housekeeping genes grouped the selected isolates into two clades, constituted by the Chilean strains, while the Canadian isolates form a branch in the phylogenetic tree. The latter consisted of two isolates that were different in both genetic and phenotypic characteristics. The phylogenies and the MLST do not reflect the origin of the isolates with respect to host species. The isolates included were heterogeneous in phenotypic tests. Conclusions The genotyping methods developed in this study provided a tool for separation of P. salmonis isolates into distinct clades. The SRS outbreaks in Chile are caused by minimum two different genetic groups of P. salmonis. This heterogeneity should be considered in future development of vaccines against this bacterium in Chile. Two different strains of P. salmonis, in regards to genetic and phenotypic characteristics, can occur in the same contemporary outbreak of SRS. Article in Journal/Newspaper Atlantic salmon DataCite Metadata Store (German National Library of Science and Technology) Canada |
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DataCite Metadata Store (German National Library of Science and Technology) |
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language |
unknown |
topic |
Medicine Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified |
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Medicine Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Otterlei, Alexander ĂYvind Brevik Jensen, Daniel Duesund, Henrik Sommerset, Ingunn Frost, Petter Mendoza, Julio McKenzie, Peter Nylund, Are Apablaza, Patricia Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids |
topic_facet |
Medicine Genetics FOS Biological sciences Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified |
description |
Abstract Background The study presents the phenotypic and genetic characterization of selected P. salmonis isolates from Atlantic salmon and rainbow trout suffering from SRS (salmonid rickettsial septicemia) in Chile and in Canada. The phenotypic characterization of the P. salmonis isolates were based on growth on different agar media (including a newly developed medium), different growth temperatures, antibiotics susceptibility and biochemical tests. Results This is the first study differentiating Chilean P. salmonis isolates into two separate genetic groups. Genotyping, based on 16S rRNA-ITS and concatenated housekeeping genes grouped the selected isolates into two clades, constituted by the Chilean strains, while the Canadian isolates form a branch in the phylogenetic tree. The latter consisted of two isolates that were different in both genetic and phenotypic characteristics. The phylogenies and the MLST do not reflect the origin of the isolates with respect to host species. The isolates included were heterogeneous in phenotypic tests. Conclusions The genotyping methods developed in this study provided a tool for separation of P. salmonis isolates into distinct clades. The SRS outbreaks in Chile are caused by minimum two different genetic groups of P. salmonis. This heterogeneity should be considered in future development of vaccines against this bacterium in Chile. Two different strains of P. salmonis, in regards to genetic and phenotypic characteristics, can occur in the same contemporary outbreak of SRS. |
format |
Article in Journal/Newspaper |
author |
Otterlei, Alexander ĂYvind Brevik Jensen, Daniel Duesund, Henrik Sommerset, Ingunn Frost, Petter Mendoza, Julio McKenzie, Peter Nylund, Are Apablaza, Patricia |
author_facet |
Otterlei, Alexander ĂYvind Brevik Jensen, Daniel Duesund, Henrik Sommerset, Ingunn Frost, Petter Mendoza, Julio McKenzie, Peter Nylund, Are Apablaza, Patricia |
author_sort |
Otterlei, Alexander |
title |
Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids |
title_short |
Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids |
title_full |
Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids |
title_fullStr |
Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids |
title_full_unstemmed |
Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids |
title_sort |
phenotypic and genetic characterization of piscirickettsia salmonis from chilean and canadian salmonids |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3628286 https://figshare.com/collections/Phenotypic_and_genetic_characterization_of_Piscirickettsia_salmonis_from_Chilean_and_Canadian_salmonids/3628286 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://dx.doi.org/10.1186/s12917-016-0681-0 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3628286 https://doi.org/10.1186/s12917-016-0681-0 |
_version_ |
1766363100709126144 |