Gene expression analysis at the onset of sex differentiation in turbot (Scophthalmus maximus)

Abstract Background Controlling sex ratios is essential for the aquaculture industry, especially in those species with sex dimorphism for relevant productive traits, hence the importance of knowing how the sexual phenotype is established in fish. Turbot, a very important fish for the aquaculture ind...

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Main Authors: Robledo, Diego, Ribas, Laia, Cal, Rosa, Sánchez, Laura, Piferrer, Francesc, Martínez, Paulino, Viñas, Ana
Format: Article in Journal/Newspaper
Language:unknown
Published: Figshare 2015
Subjects:
Online Access:https://dx.doi.org/10.6084/m9.figshare.c.3618644.v1
https://figshare.com/collections/Gene_expression_analysis_at_the_onset_of_sex_differentiation_in_turbot_Scophthalmus_maximus_/3618644/1
id ftdatacite:10.6084/m9.figshare.c.3618644.v1
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spelling ftdatacite:10.6084/m9.figshare.c.3618644.v1 2023-05-15T18:15:54+02:00 Gene expression analysis at the onset of sex differentiation in turbot (Scophthalmus maximus) Robledo, Diego Ribas, Laia Cal, Rosa Sánchez, Laura Piferrer, Francesc Martínez, Paulino Viñas, Ana 2015 https://dx.doi.org/10.6084/m9.figshare.c.3618644.v1 https://figshare.com/collections/Gene_expression_analysis_at_the_onset_of_sex_differentiation_in_turbot_Scophthalmus_maximus_/3618644/1 unknown Figshare https://dx.doi.org/10.1186/s12864-015-2142-8 https://dx.doi.org/10.6084/m9.figshare.c.3618644 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Genetics FOS Biological sciences Physiology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Marine Biology Inorganic Chemistry FOS Chemical sciences Collection article 2015 ftdatacite https://doi.org/10.6084/m9.figshare.c.3618644.v1 https://doi.org/10.1186/s12864-015-2142-8 https://doi.org/10.6084/m9.figshare.c.3618644 2021-11-05T12:55:41Z Abstract Background Controlling sex ratios is essential for the aquaculture industry, especially in those species with sex dimorphism for relevant productive traits, hence the importance of knowing how the sexual phenotype is established in fish. Turbot, a very important fish for the aquaculture industry in Europe, shows one of the largest sexual growth dimorphisms amongst marine cultured species, being all-female stocks a desirable goal for the industry. Although important knowledge has been achieved on the genetic basis of sex determination (SD) in this species, the master SD gene remains unknown and precise information on gene expression at the critical stage of sex differentiation is lacking. In the present work, we examined the expression profiles of 29 relevant genes related to sex differentiation, from 60 up to 135 days post fertilization (dpf), when gonads are differentiating. We also considered the influence of three temperature regimes on sex differentiation. Results The first sex-related differences in molecular markers could be observed at 90 days post fertilization (dpf) and so we have called that time the onset of sex differentiation. Three genes were the first to show differential expression between males and females and also allowed us to sex turbot accurately at the onset of sex differentiation (90 dpf): cyp19a1a, amh and vasa. The expression of genes related to primordial germ cells (vasa, gsdf, tdrd1) started to increase between 75–90 dpf and vasa and tdrd1 later presented higher expression in females (90-105 dpf). Two genes placed on the SD region of turbot (sox2, fxr1) did not show any expression pattern suggestive of a sex determining function. We also detected changes in the expression levels of several genes (ctnnb1, cyp11a, dmrt2 or sox6) depending on culture temperature. Conclusion Our results enabled us to identify the first sex-associated genetic cues (cyp19a1a, vasa and amh) at the initial stages of gonad development in turbot (90 dpf) and to accurately sex turbot at this age, establishing the correspondence between gene expression profiles and histological sex. Furthermore, we profiled several genes involved in sex differentiation and found specific temperature effects on their expression. Article in Journal/Newspaper Scophthalmus maximus Turbot DataCite Metadata Store (German National Library of Science and Technology) Vasa ENVELOPE(25.177,25.177,67.587,67.587)
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language unknown
topic Genetics
FOS Biological sciences
Physiology
59999 Environmental Sciences not elsewhere classified
FOS Earth and related environmental sciences
Ecology
Immunology
FOS Clinical medicine
69999 Biological Sciences not elsewhere classified
Developmental Biology
Marine Biology
Inorganic Chemistry
FOS Chemical sciences
spellingShingle Genetics
FOS Biological sciences
Physiology
59999 Environmental Sciences not elsewhere classified
FOS Earth and related environmental sciences
Ecology
Immunology
FOS Clinical medicine
69999 Biological Sciences not elsewhere classified
Developmental Biology
Marine Biology
Inorganic Chemistry
FOS Chemical sciences
Robledo, Diego
Ribas, Laia
Cal, Rosa
Sánchez, Laura
Piferrer, Francesc
Martínez, Paulino
Viñas, Ana
Gene expression analysis at the onset of sex differentiation in turbot (Scophthalmus maximus)
topic_facet Genetics
FOS Biological sciences
Physiology
59999 Environmental Sciences not elsewhere classified
FOS Earth and related environmental sciences
Ecology
Immunology
FOS Clinical medicine
69999 Biological Sciences not elsewhere classified
Developmental Biology
Marine Biology
Inorganic Chemistry
FOS Chemical sciences
description Abstract Background Controlling sex ratios is essential for the aquaculture industry, especially in those species with sex dimorphism for relevant productive traits, hence the importance of knowing how the sexual phenotype is established in fish. Turbot, a very important fish for the aquaculture industry in Europe, shows one of the largest sexual growth dimorphisms amongst marine cultured species, being all-female stocks a desirable goal for the industry. Although important knowledge has been achieved on the genetic basis of sex determination (SD) in this species, the master SD gene remains unknown and precise information on gene expression at the critical stage of sex differentiation is lacking. In the present work, we examined the expression profiles of 29 relevant genes related to sex differentiation, from 60 up to 135 days post fertilization (dpf), when gonads are differentiating. We also considered the influence of three temperature regimes on sex differentiation. Results The first sex-related differences in molecular markers could be observed at 90 days post fertilization (dpf) and so we have called that time the onset of sex differentiation. Three genes were the first to show differential expression between males and females and also allowed us to sex turbot accurately at the onset of sex differentiation (90 dpf): cyp19a1a, amh and vasa. The expression of genes related to primordial germ cells (vasa, gsdf, tdrd1) started to increase between 75–90 dpf and vasa and tdrd1 later presented higher expression in females (90-105 dpf). Two genes placed on the SD region of turbot (sox2, fxr1) did not show any expression pattern suggestive of a sex determining function. We also detected changes in the expression levels of several genes (ctnnb1, cyp11a, dmrt2 or sox6) depending on culture temperature. Conclusion Our results enabled us to identify the first sex-associated genetic cues (cyp19a1a, vasa and amh) at the initial stages of gonad development in turbot (90 dpf) and to accurately sex turbot at this age, establishing the correspondence between gene expression profiles and histological sex. Furthermore, we profiled several genes involved in sex differentiation and found specific temperature effects on their expression.
format Article in Journal/Newspaper
author Robledo, Diego
Ribas, Laia
Cal, Rosa
Sánchez, Laura
Piferrer, Francesc
Martínez, Paulino
Viñas, Ana
author_facet Robledo, Diego
Ribas, Laia
Cal, Rosa
Sánchez, Laura
Piferrer, Francesc
Martínez, Paulino
Viñas, Ana
author_sort Robledo, Diego
title Gene expression analysis at the onset of sex differentiation in turbot (Scophthalmus maximus)
title_short Gene expression analysis at the onset of sex differentiation in turbot (Scophthalmus maximus)
title_full Gene expression analysis at the onset of sex differentiation in turbot (Scophthalmus maximus)
title_fullStr Gene expression analysis at the onset of sex differentiation in turbot (Scophthalmus maximus)
title_full_unstemmed Gene expression analysis at the onset of sex differentiation in turbot (Scophthalmus maximus)
title_sort gene expression analysis at the onset of sex differentiation in turbot (scophthalmus maximus)
publisher Figshare
publishDate 2015
url https://dx.doi.org/10.6084/m9.figshare.c.3618644.v1
https://figshare.com/collections/Gene_expression_analysis_at_the_onset_of_sex_differentiation_in_turbot_Scophthalmus_maximus_/3618644/1
long_lat ENVELOPE(25.177,25.177,67.587,67.587)
geographic Vasa
geographic_facet Vasa
genre Scophthalmus maximus
Turbot
genre_facet Scophthalmus maximus
Turbot
op_relation https://dx.doi.org/10.1186/s12864-015-2142-8
https://dx.doi.org/10.6084/m9.figshare.c.3618644
op_rights CC BY 4.0
https://creativecommons.org/licenses/by/4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.6084/m9.figshare.c.3618644.v1
https://doi.org/10.1186/s12864-015-2142-8
https://doi.org/10.6084/m9.figshare.c.3618644
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