Bioprospecting potential of halogenases from Arctic marine actinomycetes ...

• Main Authors: Liao, Li, Chen, Ruiqin, Jiang, Ming, Tian, Xiaoqing, Liu, Huan, Yu, Yong, Fan, Chenqi, Chen, Bo
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2016
• Subjects: Biochemistry; Microbiology; FOS Biological sciences; Genetics; Molecular Biology; Biotechnology; Environmental Sciences not elsewhere classified; Ecology; Biological Sciences not elsewhere classified; Marine Biology; Inorganic Chemistry; FOS Chemical sciences; Arctic; Arctic Ocean
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3614015.v1; https://springernature.figshare.com/collections/Bioprospecting_potential_of_halogenases_from_Arctic_marine_actinomycetes/3614015/1

Abstract Background Halometabolites, an important group of natural products, generally require halogenases for their biosynthesis. Actinomycetes from the Arctic Ocean have rarely been investigated for halogenases and their gene clusters associated, albeit great potential of halometabolite production has been predicted. Therefore, we initiated this research on the screening of halogenases from Arctic marine actinomycetes isolates to explore their genetic potential of halometabolite biosynthesis. Results Nine halogenase genes were discovered from sixty Arctic marine actinomycetes using in-house designed or previously reported PCR primers. Four representative genotypes were further cloned to obtain full coding regions through genome walking. The resulting halogenases were predicted to be involved in halogenation of indole groups, antitumor agent ansamitocin-like substrates, or unknown peptide-like compounds. Genome sequencing revealed a potential gene cluster containing the halogenase predicted to catalyze ...