Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts
Abstract Background Amylases and cellulases have great potential for application in industries such as food, detergent, laundry, textile, baking and biofuels. A common requirement in these fields is to reduce the temperatures of the processes, leading to a continuous search for microorganisms that s...
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ftdatacite:10.6084/m9.figshare.c.3610409 2023-05-15T13:37:55+02:00 Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts Carrasco, Mario Villarreal, Pablo Barahona, Salvador Alcaíno, Jennifer Cifuentes, Víctor Baeza, Marcelo 2016 https://dx.doi.org/10.6084/m9.figshare.c.3610409 https://figshare.com/collections/Screening_and_characterization_of_amylase_and_cellulase_activities_in_psychrotolerant_yeasts/3610409 unknown Figshare https://dx.doi.org/10.1186/s12866-016-0640-8 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Science Policy 69999 Biological Sciences not elsewhere classified Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3610409 https://doi.org/10.1186/s12866-016-0640-8 2021-11-05T12:55:41Z Abstract Background Amylases and cellulases have great potential for application in industries such as food, detergent, laundry, textile, baking and biofuels. A common requirement in these fields is to reduce the temperatures of the processes, leading to a continuous search for microorganisms that secrete cold-active amylases and cellulases. Psychrotolerant yeasts are good candidates because they inhabit cold-environments. In this work, we analyzed the ability of yeasts isolated from the Antarctic region to grow on starch or carboxymethylcellulose, and their potential extracellular amylases and cellulases. Result All tested yeasts were able to grow with soluble starch or carboxymethylcellulose as the sole carbon source; however, not all of them produced ethanol by fermentation of these carbon sources. For the majority of the yeast species, the extracellular amylase or cellulase activity was higher when cultured in medium supplemented with glucose rather than with soluble starch or carboxymethylcellulose. Additionally, higher amylase activities were observed when tested at pH 5.4 and 6.2, and at 30–37 °C, except for Rhodotorula glacialis that showed elevated activity at 10–22 °C. In general, cellulase activity was high until pH 6.2 and between 22–37 °C, while the sample from Mrakia blollopis showed high activity at 4–22 °C. Peptide mass fingerprinting analysis of a potential amylase from Tetracladium sp. of about 70 kDa, showed several peptides with positive matches with glucoamylases from other fungi. Conclusions Almost all yeast species showed extracellular amylase or cellulase activity, and an inducing effect by the respective substrate was observed in a minor number of yeasts. These enzymatic activities were higher at 30 °C in most yeast, with highest amylase and cellulase activity in Tetracladium sp. and M. gelida, respectively. However, Rh. glacialis and M. blollopis displayed high amylase or cellulase activity, respectively, under 22 °C. In this sense, these yeasts are interesting candidates for industrial processes that require lower temperatures. Article in Journal/Newspaper Antarc* Antarctic DataCite Metadata Store (German National Library of Science and Technology) Antarctic The Antarctic |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Science Policy 69999 Biological Sciences not elsewhere classified |
spellingShingle |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Science Policy 69999 Biological Sciences not elsewhere classified Carrasco, Mario Villarreal, Pablo Barahona, Salvador Alcaíno, Jennifer Cifuentes, Víctor Baeza, Marcelo Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts |
topic_facet |
Biochemistry Medicine Microbiology FOS Biological sciences Cell Biology Biotechnology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology Science Policy 69999 Biological Sciences not elsewhere classified |
description |
Abstract Background Amylases and cellulases have great potential for application in industries such as food, detergent, laundry, textile, baking and biofuels. A common requirement in these fields is to reduce the temperatures of the processes, leading to a continuous search for microorganisms that secrete cold-active amylases and cellulases. Psychrotolerant yeasts are good candidates because they inhabit cold-environments. In this work, we analyzed the ability of yeasts isolated from the Antarctic region to grow on starch or carboxymethylcellulose, and their potential extracellular amylases and cellulases. Result All tested yeasts were able to grow with soluble starch or carboxymethylcellulose as the sole carbon source; however, not all of them produced ethanol by fermentation of these carbon sources. For the majority of the yeast species, the extracellular amylase or cellulase activity was higher when cultured in medium supplemented with glucose rather than with soluble starch or carboxymethylcellulose. Additionally, higher amylase activities were observed when tested at pH 5.4 and 6.2, and at 30–37 °C, except for Rhodotorula glacialis that showed elevated activity at 10–22 °C. In general, cellulase activity was high until pH 6.2 and between 22–37 °C, while the sample from Mrakia blollopis showed high activity at 4–22 °C. Peptide mass fingerprinting analysis of a potential amylase from Tetracladium sp. of about 70 kDa, showed several peptides with positive matches with glucoamylases from other fungi. Conclusions Almost all yeast species showed extracellular amylase or cellulase activity, and an inducing effect by the respective substrate was observed in a minor number of yeasts. These enzymatic activities were higher at 30 °C in most yeast, with highest amylase and cellulase activity in Tetracladium sp. and M. gelida, respectively. However, Rh. glacialis and M. blollopis displayed high amylase or cellulase activity, respectively, under 22 °C. In this sense, these yeasts are interesting candidates for industrial processes that require lower temperatures. |
format |
Article in Journal/Newspaper |
author |
Carrasco, Mario Villarreal, Pablo Barahona, Salvador Alcaíno, Jennifer Cifuentes, Víctor Baeza, Marcelo |
author_facet |
Carrasco, Mario Villarreal, Pablo Barahona, Salvador Alcaíno, Jennifer Cifuentes, Víctor Baeza, Marcelo |
author_sort |
Carrasco, Mario |
title |
Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts |
title_short |
Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts |
title_full |
Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts |
title_fullStr |
Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts |
title_full_unstemmed |
Screening and characterization of amylase and cellulase activities in psychrotolerant yeasts |
title_sort |
screening and characterization of amylase and cellulase activities in psychrotolerant yeasts |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3610409 https://figshare.com/collections/Screening_and_characterization_of_amylase_and_cellulase_activities_in_psychrotolerant_yeasts/3610409 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
https://dx.doi.org/10.1186/s12866-016-0640-8 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3610409 https://doi.org/10.1186/s12866-016-0640-8 |
_version_ |
1766099491546464256 |