Bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle
Abstract Introduction Bivalve molluscs have flourished in marine environments, and many species constitute important aquatic resources. Recently, whole genome sequences from two bivalves, the pearl oyster, Pinctada fucata, and the Pacific oyster, Crassostrea gigas, have been decoded, making it possi...
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ftdatacite:10.6084/m9.figshare.c.3610172 2023-05-15T15:59:07+02:00 Bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle Takeuchi, Takeshi Koyanagi, Ryo Fuki Gyoja Kanda, Miyuki Hisata, Kanako Fujie, Manabu Goto, Hiroki Yamasaki, Shinichi Kiyohito Nagai Morino, Yoshiaki Miyamoto, Hiroshi Endo, Kazuyoshi Endo, Hirotoshi Nagasawa, Hiromichi Shigeharu Kinoshita Asakawa, Shuichi Shugo Watabe Satoh, Noriyuki Kawashima, Takeshi 2016 https://dx.doi.org/10.6084/m9.figshare.c.3610172 https://figshare.com/collections/Bivalve-specific_gene_expansion_in_the_pearl_oyster_genome_implications_of_adaptation_to_a_sessile_lifestyle/3610172 unknown Figshare https://dx.doi.org/10.1186/s40851-016-0039-2 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biochemistry Genetics FOS Biological sciences Molecular Biology Evolutionary Biology Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences 60506 Virology Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3610172 https://doi.org/10.1186/s40851-016-0039-2 2021-11-05T12:55:41Z Abstract Introduction Bivalve molluscs have flourished in marine environments, and many species constitute important aquatic resources. Recently, whole genome sequences from two bivalves, the pearl oyster, Pinctada fucata, and the Pacific oyster, Crassostrea gigas, have been decoded, making it possible to compare genomic sequences among molluscs, and to explore general and lineage-specific genetic features and trends in bivalves. In order to improve the quality of sequence data for these purposes, we have updated the entire P. fucata genome assembly. Results We present a new genome assembly of the pearl oyster, Pinctada fucata (version 2.0). To update the assembly, we conducted additional sequencing, obtaining accumulated sequence data amounting to 193Ă the P. fucata genome. Sequence redundancy in contigs that was caused by heterozygosity was removed in silico, which significantly improved subsequent scaffolding. Gene model version 2.0 was generated with the aid of manual gene annotations supplied by the P. fucata research community. Comparison of mollusc and other bilaterian genomes shows that gene arrangements of Hox, ParaHox, and Wnt clusters in the P. fucata genome are similar to those of other molluscs. Like the Pacific oyster, P. fucata possesses many genes involved in environmental responses and in immune defense. Phylogenetic analyses of heat shock protein70 and C1q domain-containing protein families indicate that extensive expansion of genes occurred independently in each lineage. Several gene duplication events prior to the split between the pearl oyster and the Pacific oyster are also evident. In addition, a number of tandem duplications of genes that encode shell matrix proteins are also well characterized in the P. fucata genome. Conclusions Both the Pinctada and Crassostrea lineages have expanded specific gene families in a lineage-specific manner. Frequent duplication of genes responsible for shell formation in the P. fucata genome explains the diversity of mollusc shell structures. These duplications reveal dynamic genome evolution to forge the complex physiology that enables bivalves to employ a sessile lifestyle in the intertidal zone. Article in Journal/Newspaper Crassostrea gigas Pacific oyster DataCite Metadata Store (German National Library of Science and Technology) Pacific |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Biochemistry Genetics FOS Biological sciences Molecular Biology Evolutionary Biology Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences 60506 Virology |
spellingShingle |
Biochemistry Genetics FOS Biological sciences Molecular Biology Evolutionary Biology Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences 60506 Virology Takeuchi, Takeshi Koyanagi, Ryo Fuki Gyoja Kanda, Miyuki Hisata, Kanako Fujie, Manabu Goto, Hiroki Yamasaki, Shinichi Kiyohito Nagai Morino, Yoshiaki Miyamoto, Hiroshi Endo, Kazuyoshi Endo, Hirotoshi Nagasawa, Hiromichi Shigeharu Kinoshita Asakawa, Shuichi Shugo Watabe Satoh, Noriyuki Kawashima, Takeshi Bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle |
topic_facet |
Biochemistry Genetics FOS Biological sciences Molecular Biology Evolutionary Biology Ecology 69999 Biological Sciences not elsewhere classified Marine Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences 60506 Virology |
description |
Abstract Introduction Bivalve molluscs have flourished in marine environments, and many species constitute important aquatic resources. Recently, whole genome sequences from two bivalves, the pearl oyster, Pinctada fucata, and the Pacific oyster, Crassostrea gigas, have been decoded, making it possible to compare genomic sequences among molluscs, and to explore general and lineage-specific genetic features and trends in bivalves. In order to improve the quality of sequence data for these purposes, we have updated the entire P. fucata genome assembly. Results We present a new genome assembly of the pearl oyster, Pinctada fucata (version 2.0). To update the assembly, we conducted additional sequencing, obtaining accumulated sequence data amounting to 193Ă the P. fucata genome. Sequence redundancy in contigs that was caused by heterozygosity was removed in silico, which significantly improved subsequent scaffolding. Gene model version 2.0 was generated with the aid of manual gene annotations supplied by the P. fucata research community. Comparison of mollusc and other bilaterian genomes shows that gene arrangements of Hox, ParaHox, and Wnt clusters in the P. fucata genome are similar to those of other molluscs. Like the Pacific oyster, P. fucata possesses many genes involved in environmental responses and in immune defense. Phylogenetic analyses of heat shock protein70 and C1q domain-containing protein families indicate that extensive expansion of genes occurred independently in each lineage. Several gene duplication events prior to the split between the pearl oyster and the Pacific oyster are also evident. In addition, a number of tandem duplications of genes that encode shell matrix proteins are also well characterized in the P. fucata genome. Conclusions Both the Pinctada and Crassostrea lineages have expanded specific gene families in a lineage-specific manner. Frequent duplication of genes responsible for shell formation in the P. fucata genome explains the diversity of mollusc shell structures. These duplications reveal dynamic genome evolution to forge the complex physiology that enables bivalves to employ a sessile lifestyle in the intertidal zone. |
format |
Article in Journal/Newspaper |
author |
Takeuchi, Takeshi Koyanagi, Ryo Fuki Gyoja Kanda, Miyuki Hisata, Kanako Fujie, Manabu Goto, Hiroki Yamasaki, Shinichi Kiyohito Nagai Morino, Yoshiaki Miyamoto, Hiroshi Endo, Kazuyoshi Endo, Hirotoshi Nagasawa, Hiromichi Shigeharu Kinoshita Asakawa, Shuichi Shugo Watabe Satoh, Noriyuki Kawashima, Takeshi |
author_facet |
Takeuchi, Takeshi Koyanagi, Ryo Fuki Gyoja Kanda, Miyuki Hisata, Kanako Fujie, Manabu Goto, Hiroki Yamasaki, Shinichi Kiyohito Nagai Morino, Yoshiaki Miyamoto, Hiroshi Endo, Kazuyoshi Endo, Hirotoshi Nagasawa, Hiromichi Shigeharu Kinoshita Asakawa, Shuichi Shugo Watabe Satoh, Noriyuki Kawashima, Takeshi |
author_sort |
Takeuchi, Takeshi |
title |
Bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle |
title_short |
Bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle |
title_full |
Bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle |
title_fullStr |
Bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle |
title_full_unstemmed |
Bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle |
title_sort |
bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3610172 https://figshare.com/collections/Bivalve-specific_gene_expansion_in_the_pearl_oyster_genome_implications_of_adaptation_to_a_sessile_lifestyle/3610172 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://dx.doi.org/10.1186/s40851-016-0039-2 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3610172 https://doi.org/10.1186/s40851-016-0039-2 |
_version_ |
1766394903550492672 |