Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum
Abstract A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the cre...
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Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.3608933.v1 https://figshare.com/collections/Complete_genome_sequence_of_and_proposal_of_Thermofilum_uzonense_sp_nov_a_novel_hyperthermophilic_crenarchaeon_and_emended_description_of_the_genus_Thermofilum/3608933/1 |
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ftdatacite:10.6084/m9.figshare.c.3608933.v1 2023-05-15T16:59:20+02:00 Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum Toshchakov, Stepan Korzhenkov, Aleksei Samarov, Nazar Mazunin, Ilia Mozhey, Oleg Shmyr, Ilya Derbikova, Ksenia Taranov, Evgeny Dominova, Irina Bonch-Osmolovskaya, Elizaveta Patrushev, Maxim Podosokorskaya, Olga Kublanov, Ilya 2015 https://dx.doi.org/10.6084/m9.figshare.c.3608933.v1 https://figshare.com/collections/Complete_genome_sequence_of_and_proposal_of_Thermofilum_uzonense_sp_nov_a_novel_hyperthermophilic_crenarchaeon_and_emended_description_of_the_genus_Thermofilum/3608933/1 unknown Figshare https://dx.doi.org/10.1186/s40793-015-0105-y https://dx.doi.org/10.6084/m9.figshare.c.3608933 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Space Science Microbiology FOS Biological sciences Genetics Molecular Biology Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 110309 Infectious Diseases FOS Health sciences 60506 Virology Computational Biology Collection article 2015 ftdatacite https://doi.org/10.6084/m9.figshare.c.3608933.v1 https://doi.org/10.1186/s40793-015-0105-y https://doi.org/10.6084/m9.figshare.c.3608933 2021-11-05T12:55:41Z Abstract A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal genus Thermofilum; Thermofilum pendens was its closest valid relative (95.7 % of sequence identity). Strain 1807-2 grew organothrophically using polysaccharides (starch and glucomannan), yeast extract or peptone as substrates. The addition of other crenarchaea culture broth filtrates was obligatory required for growth and could not be replaced by the addition of these organisms’ cell wall fractions, as it was described for T. pendens. The genome of strain 1807-2 was sequenced using Illumina and PGM technologies. The average nucleotide identities between genome of strain 1807-2 and T. pendens strain HRK 5T and “T. adornatus” strain 1910b were 85 and 82 %, respectively. On the basis of 16S rRNA gene sequence phylogeny, ANI calculations and phenotypic differences we propose a novel species Thermofilum uzonense with the type strain 1807-2T (= DSM 28062T = JCM 19810T). Project information and genome sequence was deposited in Genbank under IDs PRJNA262459 and CP009961, respectively. Article in Journal/Newspaper Kamchatka DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Space Science Microbiology FOS Biological sciences Genetics Molecular Biology Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 110309 Infectious Diseases FOS Health sciences 60506 Virology Computational Biology |
spellingShingle |
Space Science Microbiology FOS Biological sciences Genetics Molecular Biology Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 110309 Infectious Diseases FOS Health sciences 60506 Virology Computational Biology Toshchakov, Stepan Korzhenkov, Aleksei Samarov, Nazar Mazunin, Ilia Mozhey, Oleg Shmyr, Ilya Derbikova, Ksenia Taranov, Evgeny Dominova, Irina Bonch-Osmolovskaya, Elizaveta Patrushev, Maxim Podosokorskaya, Olga Kublanov, Ilya Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
topic_facet |
Space Science Microbiology FOS Biological sciences Genetics Molecular Biology Evolutionary Biology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified 110309 Infectious Diseases FOS Health sciences 60506 Virology Computational Biology |
description |
Abstract A strain of a hyperthermophilic filamentous archaeon was isolated from a sample of Kamchatka hot spring sediment. Isolate 1807-2 grew optimally at 85 °C, pH 6.0-6.5, the parameters being close to those at the sampling site. 16S rRNA gene sequence analysis placed the novel isolate in the crenarchaeal genus Thermofilum; Thermofilum pendens was its closest valid relative (95.7 % of sequence identity). Strain 1807-2 grew organothrophically using polysaccharides (starch and glucomannan), yeast extract or peptone as substrates. The addition of other crenarchaea culture broth filtrates was obligatory required for growth and could not be replaced by the addition of these organisms’ cell wall fractions, as it was described for T. pendens. The genome of strain 1807-2 was sequenced using Illumina and PGM technologies. The average nucleotide identities between genome of strain 1807-2 and T. pendens strain HRK 5T and “T. adornatus” strain 1910b were 85 and 82 %, respectively. On the basis of 16S rRNA gene sequence phylogeny, ANI calculations and phenotypic differences we propose a novel species Thermofilum uzonense with the type strain 1807-2T (= DSM 28062T = JCM 19810T). Project information and genome sequence was deposited in Genbank under IDs PRJNA262459 and CP009961, respectively. |
format |
Article in Journal/Newspaper |
author |
Toshchakov, Stepan Korzhenkov, Aleksei Samarov, Nazar Mazunin, Ilia Mozhey, Oleg Shmyr, Ilya Derbikova, Ksenia Taranov, Evgeny Dominova, Irina Bonch-Osmolovskaya, Elizaveta Patrushev, Maxim Podosokorskaya, Olga Kublanov, Ilya |
author_facet |
Toshchakov, Stepan Korzhenkov, Aleksei Samarov, Nazar Mazunin, Ilia Mozhey, Oleg Shmyr, Ilya Derbikova, Ksenia Taranov, Evgeny Dominova, Irina Bonch-Osmolovskaya, Elizaveta Patrushev, Maxim Podosokorskaya, Olga Kublanov, Ilya |
author_sort |
Toshchakov, Stepan |
title |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_short |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_full |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_fullStr |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_full_unstemmed |
Complete genome sequence of and proposal of Thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus Thermofilum |
title_sort |
complete genome sequence of and proposal of thermofilum uzonense sp. nov. a novel hyperthermophilic crenarchaeon and emended description of the genus thermofilum |
publisher |
Figshare |
publishDate |
2015 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3608933.v1 https://figshare.com/collections/Complete_genome_sequence_of_and_proposal_of_Thermofilum_uzonense_sp_nov_a_novel_hyperthermophilic_crenarchaeon_and_emended_description_of_the_genus_Thermofilum/3608933/1 |
genre |
Kamchatka |
genre_facet |
Kamchatka |
op_relation |
https://dx.doi.org/10.1186/s40793-015-0105-y https://dx.doi.org/10.6084/m9.figshare.c.3608933 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3608933.v1 https://doi.org/10.1186/s40793-015-0105-y https://doi.org/10.6084/m9.figshare.c.3608933 |
_version_ |
1766051578910867456 |