The comprehensive immunomodulation of NeurimmiRs in haemocytes of oyster Crassostrea gigas after acetylcholine and norepinephrine stimulation ...

• Main Authors: Chen, Hao, Wang, Lingling, Zhou, Zhi, Hou, Zhanhui, Liu, Zhaoqun, Wang, Weilin, Gao, Dahai, Gao, Qiang, Wang, Mengqiang, Song, Linsheng
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: figshare 2015
• Subjects: Medicine; Genetics; FOS Biological sciences; Molecular Biology; Physiology; Immunology; FOS Clinical medicine; Biological Sciences not elsewhere classified; Science Policy; Crassostrea gigas
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.3605885.v1; https://springernature.figshare.com/collections/The_comprehensive_immunomodulation_of_NeurimmiRs_in_haemocytes_of_oyster_Crassostrea_gigas_after_acetylcholine_and_norepinephrine_stimulation/3605885/1

Abstract Background Neural-endocrine-immune (NEI) system is a major modulation network among the nervous, endocrine and immune system and weights greatly in maintaining homeostasis of organisms during stress and infection. Some microRNAs are found interacting with NEI system (designated NeurimmiRs), addressing swift modulations on immune system. The oyster Crassostrea gigas, as an intertidal bivalve, has evolved a primary NEI system. However, the knowledge about NeurimmiRs in oysters remains largely unknown. Results Six small RNA libraries from haemocytes of oysters stimulated with acetylcholine (ACh) and norepinephrine (NE) were sequenced to identify neurotransmitter-responsive miRNAs and survey their immunomodulation roles. A total of 331 miRNAs (132 identified in the present study plus 199 identified previously) were subjected to expression analysis, and twenty-one and sixteen of them were found ACh- or NE-responsive, respectively (FDR < 0.05). Meanwhile, 21 miRNAs exhibited different expression ...