Dynamics of nuclear receptor gene expression during Pacific oyster development
Abstract Background Nuclear receptors are a highly conserved set of ligand binding transcription factors, with essential roles regulating aspects of vertebrate and invertebrate biology alike. Current understanding of nuclear receptor regulated gene expression in invertebrates remains sparse, limitin...
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ftdatacite:10.6084/m9.figshare.c.3602864.v1 2023-05-15T15:59:03+02:00 Dynamics of nuclear receptor gene expression during Pacific oyster development Vogeler, Susanne Bean, Tim Lyons, Brett Galloway, Tamara 2016 https://dx.doi.org/10.6084/m9.figshare.c.3602864.v1 https://figshare.com/collections/Dynamics_of_nuclear_receptor_gene_expression_during_Pacific_oyster_development/3602864/1 unknown Figshare https://dx.doi.org/10.1186/s12861-016-0129-6 https://dx.doi.org/10.6084/m9.figshare.c.3602864 CC BY 4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Biophysics Medicine Cell Biology Genetics FOS Biological sciences Molecular Biology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Computational Biology Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3602864.v1 https://doi.org/10.1186/s12861-016-0129-6 https://doi.org/10.6084/m9.figshare.c.3602864 2021-11-05T12:55:41Z Abstract Background Nuclear receptors are a highly conserved set of ligand binding transcription factors, with essential roles regulating aspects of vertebrate and invertebrate biology alike. Current understanding of nuclear receptor regulated gene expression in invertebrates remains sparse, limiting our ability to elucidate gene function and the conservation of developmental processes across phyla. Here, we studied nuclear receptor expression in the early life stages of the Pacific oyster, Crassostrea gigas, to identify at which specific key stages nuclear receptors are expressed Results We used quantitative RT-PCR to determine the expression profiles of 34 nuclear receptors, revealing three developmental key stages, during which nuclear receptor expression is dynamically regulated: embryogenesis, mid development from gastrulation to trochophore larva, and late larval development prior to metamorphosis. Clustering of nuclear receptor expression patterns demonstrated that transcriptional regulation was not directly related to gene phylogeny, suggesting closely related genes may have distinct functions. Expression of gene homologs of vertebrate retinoid receptors suggests participation in organogenesis and shell-formation, as they are highly expressed at the gastrulation and trochophore larval initial shell formation stages. The ecdysone receptor homolog showed high expression just before larval settlement, suggesting a potential role in metamorphosis. Conclusion Throughout early oyster development nuclear receptors exhibited highly dynamic expression profiles, which were not confined by gene phylogeny. These results provide fundamental information on the presence of nuclear receptors during key developmental stages, which aids elucidation of their function in the developmental process. This understanding is essential as ligand sensing nuclear receptors can be disrupted by xenobiotics, a mode of action through which anthropogenic environmental pollutants have been found to mediate effects. Article in Journal/Newspaper Crassostrea gigas Pacific oyster DataCite Metadata Store (German National Library of Science and Technology) Pacific |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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language |
unknown |
topic |
Biophysics Medicine Cell Biology Genetics FOS Biological sciences Molecular Biology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Computational Biology |
spellingShingle |
Biophysics Medicine Cell Biology Genetics FOS Biological sciences Molecular Biology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Computational Biology Vogeler, Susanne Bean, Tim Lyons, Brett Galloway, Tamara Dynamics of nuclear receptor gene expression during Pacific oyster development |
topic_facet |
Biophysics Medicine Cell Biology Genetics FOS Biological sciences Molecular Biology Immunology FOS Clinical medicine 69999 Biological Sciences not elsewhere classified Developmental Biology Inorganic Chemistry FOS Chemical sciences 110309 Infectious Diseases FOS Health sciences Computational Biology |
description |
Abstract Background Nuclear receptors are a highly conserved set of ligand binding transcription factors, with essential roles regulating aspects of vertebrate and invertebrate biology alike. Current understanding of nuclear receptor regulated gene expression in invertebrates remains sparse, limiting our ability to elucidate gene function and the conservation of developmental processes across phyla. Here, we studied nuclear receptor expression in the early life stages of the Pacific oyster, Crassostrea gigas, to identify at which specific key stages nuclear receptors are expressed Results We used quantitative RT-PCR to determine the expression profiles of 34 nuclear receptors, revealing three developmental key stages, during which nuclear receptor expression is dynamically regulated: embryogenesis, mid development from gastrulation to trochophore larva, and late larval development prior to metamorphosis. Clustering of nuclear receptor expression patterns demonstrated that transcriptional regulation was not directly related to gene phylogeny, suggesting closely related genes may have distinct functions. Expression of gene homologs of vertebrate retinoid receptors suggests participation in organogenesis and shell-formation, as they are highly expressed at the gastrulation and trochophore larval initial shell formation stages. The ecdysone receptor homolog showed high expression just before larval settlement, suggesting a potential role in metamorphosis. Conclusion Throughout early oyster development nuclear receptors exhibited highly dynamic expression profiles, which were not confined by gene phylogeny. These results provide fundamental information on the presence of nuclear receptors during key developmental stages, which aids elucidation of their function in the developmental process. This understanding is essential as ligand sensing nuclear receptors can be disrupted by xenobiotics, a mode of action through which anthropogenic environmental pollutants have been found to mediate effects. |
format |
Article in Journal/Newspaper |
author |
Vogeler, Susanne Bean, Tim Lyons, Brett Galloway, Tamara |
author_facet |
Vogeler, Susanne Bean, Tim Lyons, Brett Galloway, Tamara |
author_sort |
Vogeler, Susanne |
title |
Dynamics of nuclear receptor gene expression during Pacific oyster development |
title_short |
Dynamics of nuclear receptor gene expression during Pacific oyster development |
title_full |
Dynamics of nuclear receptor gene expression during Pacific oyster development |
title_fullStr |
Dynamics of nuclear receptor gene expression during Pacific oyster development |
title_full_unstemmed |
Dynamics of nuclear receptor gene expression during Pacific oyster development |
title_sort |
dynamics of nuclear receptor gene expression during pacific oyster development |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3602864.v1 https://figshare.com/collections/Dynamics_of_nuclear_receptor_gene_expression_during_Pacific_oyster_development/3602864/1 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
https://dx.doi.org/10.1186/s12861-016-0129-6 https://dx.doi.org/10.6084/m9.figshare.c.3602864 |
op_rights |
CC BY 4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3602864.v1 https://doi.org/10.1186/s12861-016-0129-6 https://doi.org/10.6084/m9.figshare.c.3602864 |
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1766394828614008832 |