Supplementary material from " In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p CO 2 vent sites"
Laboratory experiments suggest that calcifying developmental stages of marine invertebrates may be the most ocean acidification (OA)-sensitive life-history stage and represent a life-history bottleneck. To better extrapolate laboratory findings to future OA conditions, developmental responses in sea...
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Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.3581426.v1 https://figshare.com/collections/Supplementary_material_from_i_In_situ_i_developmental_responses_of_tropical_sea_urchin_embryos_to_ocean_acidification_conditions_at_naturally_elevated_i_p_i_CO_sub_2_sub_vent_sites_/3581426/1 |
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ftdatacite:10.6084/m9.figshare.c.3581426.v1 2023-05-15T17:50:50+02:00 Supplementary material from " In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p CO 2 vent sites" Lamare, Miles Liddy, Michelle Uthicke, Sven 2016 https://dx.doi.org/10.6084/m9.figshare.c.3581426.v1 https://figshare.com/collections/Supplementary_material_from_i_In_situ_i_developmental_responses_of_tropical_sea_urchin_embryos_to_ocean_acidification_conditions_at_naturally_elevated_i_p_i_CO_sub_2_sub_vent_sites_/3581426/1 unknown Figshare https://dx.doi.org/10.1098/rspb.2016.1506 https://dx.doi.org/10.6084/m9.figshare.c.3581426 CC-BY https://creativecommons.org/licenses/by/4.0 CC-BY Environmental Science Ecology FOS Biological sciences Developmental Biology Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3581426.v1 https://doi.org/10.1098/rspb.2016.1506 https://doi.org/10.6084/m9.figshare.c.3581426 2021-11-05T12:55:41Z Laboratory experiments suggest that calcifying developmental stages of marine invertebrates may be the most ocean acidification (OA)-sensitive life-history stage and represent a life-history bottleneck. To better extrapolate laboratory findings to future OA conditions, developmental responses in sea urchin embryos were compared under ecologically relevant in situ exposures on vent-elevated p CO 2 and ambient p CO 2 coral reefs in Papua New Guinea. Echinometra embryos/larvae were reared in meshed chambers moored in arrays on either venting reefs or adjacent non-vent reefs. After 24 and 48 h, larval development and morphology was quantified. Compared with controls (mean pH (T) = 7.89–7.92), embryos developing in elevated p CO 2 vent conditions (pH (T) = 7.50–7.72) displayed a significant reduction in size and increased abnormality, with a significant correlation of seawater pH with both larval size and larval asymmetry across all experiments. Reciprocal transplants (embryos from vent adults transplanted to control conditions, and vice versa ) were also undertaken to identify if adult acclimatization can translate resilience to offspring (i.e. transgenerational processes). Embryos originating from vent adults were, however, no more tolerant to reduced pH. Sea temperature and chlorophyll- a concentrations (i.e. larval nutrition) did not contribute to difference in larval size, but abnormality was correlated with chlorophyll levels. This study is the first to examine the response of marine larvae to OA scenarios in the natural environment where, importantly, we found that stunted and abnormal development observed in situ is consistent with laboratory observations reported in sea urchins, in both the direction and magnitude of the response. Article in Journal/Newspaper Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
Environmental Science Ecology FOS Biological sciences Developmental Biology |
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Environmental Science Ecology FOS Biological sciences Developmental Biology Lamare, Miles Liddy, Michelle Uthicke, Sven Supplementary material from " In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p CO 2 vent sites" |
topic_facet |
Environmental Science Ecology FOS Biological sciences Developmental Biology |
description |
Laboratory experiments suggest that calcifying developmental stages of marine invertebrates may be the most ocean acidification (OA)-sensitive life-history stage and represent a life-history bottleneck. To better extrapolate laboratory findings to future OA conditions, developmental responses in sea urchin embryos were compared under ecologically relevant in situ exposures on vent-elevated p CO 2 and ambient p CO 2 coral reefs in Papua New Guinea. Echinometra embryos/larvae were reared in meshed chambers moored in arrays on either venting reefs or adjacent non-vent reefs. After 24 and 48 h, larval development and morphology was quantified. Compared with controls (mean pH (T) = 7.89–7.92), embryos developing in elevated p CO 2 vent conditions (pH (T) = 7.50–7.72) displayed a significant reduction in size and increased abnormality, with a significant correlation of seawater pH with both larval size and larval asymmetry across all experiments. Reciprocal transplants (embryos from vent adults transplanted to control conditions, and vice versa ) were also undertaken to identify if adult acclimatization can translate resilience to offspring (i.e. transgenerational processes). Embryos originating from vent adults were, however, no more tolerant to reduced pH. Sea temperature and chlorophyll- a concentrations (i.e. larval nutrition) did not contribute to difference in larval size, but abnormality was correlated with chlorophyll levels. This study is the first to examine the response of marine larvae to OA scenarios in the natural environment where, importantly, we found that stunted and abnormal development observed in situ is consistent with laboratory observations reported in sea urchins, in both the direction and magnitude of the response. |
format |
Article in Journal/Newspaper |
author |
Lamare, Miles Liddy, Michelle Uthicke, Sven |
author_facet |
Lamare, Miles Liddy, Michelle Uthicke, Sven |
author_sort |
Lamare, Miles |
title |
Supplementary material from " In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p CO 2 vent sites" |
title_short |
Supplementary material from " In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p CO 2 vent sites" |
title_full |
Supplementary material from " In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p CO 2 vent sites" |
title_fullStr |
Supplementary material from " In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p CO 2 vent sites" |
title_full_unstemmed |
Supplementary material from " In situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p CO 2 vent sites" |
title_sort |
supplementary material from " in situ developmental responses of tropical sea urchin embryos to ocean acidification conditions at naturally elevated p co 2 vent sites" |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3581426.v1 https://figshare.com/collections/Supplementary_material_from_i_In_situ_i_developmental_responses_of_tropical_sea_urchin_embryos_to_ocean_acidification_conditions_at_naturally_elevated_i_p_i_CO_sub_2_sub_vent_sites_/3581426/1 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1098/rspb.2016.1506 https://dx.doi.org/10.6084/m9.figshare.c.3581426 |
op_rights |
CC-BY https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3581426.v1 https://doi.org/10.1098/rspb.2016.1506 https://doi.org/10.6084/m9.figshare.c.3581426 |
_version_ |
1766157736023687168 |