Role and expression of cry1 in the adductor muscle of the oyster Crassostrea gigas during daily and tidal valve activity rhythms
Cryptochromes are flavin- and pterin-containing photoreceptors of the cryptochrome/photolyase family. They play critical roles in organisms, among are which light-dependent and light-independent roles in biological rhythms. The present work aimed at describing a cryptochrome gene in the oyster Crass...
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ftdatacite:10.6084/m9.figshare.c.3262924.v1 2023-05-15T15:58:12+02:00 Role and expression of cry1 in the adductor muscle of the oyster Crassostrea gigas during daily and tidal valve activity rhythms Mat, Audrey M. Perrigault, Mickael Jean-Charles Massabuau Tran, Damien 2016 https://dx.doi.org/10.6084/m9.figshare.c.3262924.v1 https://figshare.com/collections/Role_and_expression_of_i_cry1_i_in_the_adductor_muscle_of_the_oyster_i_Crassostrea_gigas_i_during_daily_and_tidal_valve_activity_rhythms/3262924/1 unknown Figshare https://dx.doi.org/10.1080/07420528.2016.1181645 https://dx.doi.org/10.6084/m9.figshare.c.3262924 CC-BY http://creativecommons.org/licenses/by/3.0/us CC-BY Genetics FOS Biological sciences Molecular Biology Physiology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Science Policy Collection article 2016 ftdatacite https://doi.org/10.6084/m9.figshare.c.3262924.v1 https://doi.org/10.1080/07420528.2016.1181645 https://doi.org/10.6084/m9.figshare.c.3262924 2021-11-05T12:55:41Z Cryptochromes are flavin- and pterin-containing photoreceptors of the cryptochrome/photolyase family. They play critical roles in organisms, among are which light-dependent and light-independent roles in biological rhythms. The present work aimed at describing a cryptochrome gene in the oyster Crassostrea gigas by (i) a characterization and phylogenetic analysis and (ii) by studying its expression in the relationship to rhythmic valve behavior in different entrainment regimes. Cryptochrome expression was focused on the adductor muscle of the oyster, the effector of the valve behavior. The results suggest involvement of Cgcry1 in oyster rhythmicity as a sensor of environmental zeitgebers, associated with circadian rhythms and potentially to tidal activity. The characterized gene belongs to type 1 cryptochrome/insect-type cry. Additionally, Cgcry1 presented a daily oscillation under L:D entrainment, which disappeared in constant darkness. Transcript expression of Cgcry1 also oscillated at tidal frequency under tidal entrainment and in constant darkness. Finally, exposure of tidally entrained oysters to saxitoxin (STX)-producing alga Alexandrium minutum induced a dose effect response in oysters by first altering Cgcry1 expression and then the behavior of oysters with increasing concentrations of toxins. This study initiates the characterization of the molecular clock in the oyster C. gigas and its interactions with environmental zeitgebers. Article in Journal/Newspaper Crassostrea gigas DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Genetics FOS Biological sciences Molecular Biology Physiology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Science Policy |
spellingShingle |
Genetics FOS Biological sciences Molecular Biology Physiology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Science Policy Mat, Audrey M. Perrigault, Mickael Jean-Charles Massabuau Tran, Damien Role and expression of cry1 in the adductor muscle of the oyster Crassostrea gigas during daily and tidal valve activity rhythms |
topic_facet |
Genetics FOS Biological sciences Molecular Biology Physiology 59999 Environmental Sciences not elsewhere classified FOS Earth and related environmental sciences Ecology 69999 Biological Sciences not elsewhere classified Inorganic Chemistry FOS Chemical sciences Science Policy |
description |
Cryptochromes are flavin- and pterin-containing photoreceptors of the cryptochrome/photolyase family. They play critical roles in organisms, among are which light-dependent and light-independent roles in biological rhythms. The present work aimed at describing a cryptochrome gene in the oyster Crassostrea gigas by (i) a characterization and phylogenetic analysis and (ii) by studying its expression in the relationship to rhythmic valve behavior in different entrainment regimes. Cryptochrome expression was focused on the adductor muscle of the oyster, the effector of the valve behavior. The results suggest involvement of Cgcry1 in oyster rhythmicity as a sensor of environmental zeitgebers, associated with circadian rhythms and potentially to tidal activity. The characterized gene belongs to type 1 cryptochrome/insect-type cry. Additionally, Cgcry1 presented a daily oscillation under L:D entrainment, which disappeared in constant darkness. Transcript expression of Cgcry1 also oscillated at tidal frequency under tidal entrainment and in constant darkness. Finally, exposure of tidally entrained oysters to saxitoxin (STX)-producing alga Alexandrium minutum induced a dose effect response in oysters by first altering Cgcry1 expression and then the behavior of oysters with increasing concentrations of toxins. This study initiates the characterization of the molecular clock in the oyster C. gigas and its interactions with environmental zeitgebers. |
format |
Article in Journal/Newspaper |
author |
Mat, Audrey M. Perrigault, Mickael Jean-Charles Massabuau Tran, Damien |
author_facet |
Mat, Audrey M. Perrigault, Mickael Jean-Charles Massabuau Tran, Damien |
author_sort |
Mat, Audrey M. |
title |
Role and expression of cry1 in the adductor muscle of the oyster Crassostrea gigas during daily and tidal valve activity rhythms |
title_short |
Role and expression of cry1 in the adductor muscle of the oyster Crassostrea gigas during daily and tidal valve activity rhythms |
title_full |
Role and expression of cry1 in the adductor muscle of the oyster Crassostrea gigas during daily and tidal valve activity rhythms |
title_fullStr |
Role and expression of cry1 in the adductor muscle of the oyster Crassostrea gigas during daily and tidal valve activity rhythms |
title_full_unstemmed |
Role and expression of cry1 in the adductor muscle of the oyster Crassostrea gigas during daily and tidal valve activity rhythms |
title_sort |
role and expression of cry1 in the adductor muscle of the oyster crassostrea gigas during daily and tidal valve activity rhythms |
publisher |
Figshare |
publishDate |
2016 |
url |
https://dx.doi.org/10.6084/m9.figshare.c.3262924.v1 https://figshare.com/collections/Role_and_expression_of_i_cry1_i_in_the_adductor_muscle_of_the_oyster_i_Crassostrea_gigas_i_during_daily_and_tidal_valve_activity_rhythms/3262924/1 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
https://dx.doi.org/10.1080/07420528.2016.1181645 https://dx.doi.org/10.6084/m9.figshare.c.3262924 |
op_rights |
CC-BY http://creativecommons.org/licenses/by/3.0/us |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.6084/m9.figshare.c.3262924.v1 https://doi.org/10.1080/07420528.2016.1181645 https://doi.org/10.6084/m9.figshare.c.3262924 |
_version_ |
1766393931821481984 |