Developmental effects of two different copper oxide nanomaterials in sea urchin ( Lytechinus pictus ) embryos
Copper oxide nanomaterials (nano-CuOs) are widely used and can be inadvertently introduced into estuarine and marine environments. We analyzed the effects of different nano-CuOs (a synthesized and a less-pure commercial form), as well as ionic copper (CuSO 4 ) on embryo development in the white sea...
Main Authors: | , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Figshare
2016
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Subjects: | |
Online Access: | https://dx.doi.org/10.6084/m9.figshare.c.3248935.v1 https://figshare.com/collections/Developmental_effects_of_two_different_copper_oxide_nanomaterials_in_sea_urchin_i_Lytechinus_pictus_i_embryos/3248935/1 |
Summary: | Copper oxide nanomaterials (nano-CuOs) are widely used and can be inadvertently introduced into estuarine and marine environments. We analyzed the effects of different nano-CuOs (a synthesized and a less-pure commercial form), as well as ionic copper (CuSO 4 ) on embryo development in the white sea urchin, a well-known marine model. After 96 h of development with both nano-CuO exposures, we did not detect significant oxidative damage to proteins but did detect decreases in total antioxidant capacity. We show that the physicochemical characteristics of the two nano-CuOs play an essential role in their toxicities. Both nano-CuOs were internalized by embryos and their differential dissolution was the most important toxicological parameter. The synthesized nano-CuO showed greater toxicity (EC 50 = 450 ppb of copper) and had increased dissolution (2.5% by weight over 96 h) as compared with the less-pure commercial nano-CuO (EC 50 = 5395 ppb of copper, 0.73% dissolution by weight over 96 h). Copper caused specific developmental abnormalities in sea urchin embryos including disruption of the aboral-oral axis as a result in changes to the redox environment caused by dissolution of internalized nano-CuO. Abnormal skeleton formation also occurred. |
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