Cryoprotective properties and preliminary characterization of exopolysaccharide (P-Arcpo 15) produced by the Arctic bacterium Pseudoalteromonas elyakovii Arcpo 15

• Main Authors: Kim, Sung Jin, Byung-Gee Kim, Park, Ha Ju, Yim, Joung Han
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Figshare 2016
• Subjects: Biophysics; Biochemistry; Microbiology; FOS Biological sciences; Biotechnology; 59999 Environmental Sciences not elsewhere classified; FOS Earth and related environmental sciences; 39999 Chemical Sciences not elsewhere classified; FOS Chemical sciences; Ecology; 69999 Biological Sciences not elsewhere classified; Cancer; Inorganic Chemistry; Arctic; Arctic Ocean; Chukchi Sea; Chukchi
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.2178972; https://figshare.com/collections/Cryoprotective_Properties_and_Preliminary_Characterization_of_Exopolysaccharide_P_ArcPo_15_Produced_by_the_Arctic_Bacterium_i_Pseudoalteromonas_elyakovii_i_ArcPo_15/2178972/2

Twenty-two bacterial strains that secrete exopolysaccharides (EPS) were isolated from marine samples obtained from the Chukchi Sea in the Arctic Ocean; of these, seven strains were found to be capable of producing cryoprotective EPS. The ArcPo 15 strain was isolated based on its ability to secrete large amounts of EPS, and was identified as Pseudoalteromonas elyakovii based on 16S rDNA analysis. The EPS, P-ArcPo 15, was purified by protease treatment and gel filtration chromatography. The purified EPS (P-ArcPo 15) had a molecular mass of 1.7 × 10 7 Da, and its infrared spectrum showed absorption bands of hydroxyl and carboxyl groups. The principal sugar components of P-ArcPo 15 were determined to be mannose and galacturonic acid, in the ratio of 3.3:1.0. The cryoprotective properties of P-ArcPo 15 were characterized by an Escherichia coli viability test. In the presence of 0.5% (w/v) EPS, the survival percentage of E. coli cells was as high as 94.19 ± 7.81% over five repeated freeze–thaw cycles. These biochemical characteristics suggest that the EPS P-ArcPo 15 may be useful in the development of cryoprotectants for biotechnological purposes, and we therefore assessed the utility of this novel cryoprotective EPS.