The sperm proteome of the Pacific oyster Crassostrea gigas and immunolocalization of heat shock proteins ...

• Main Authors: Huang, Xiaohong, Liu, Baozhong, Huan, Pin
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Taylor & Francis 2015
• Subjects: Hematology; Inorganic Chemistry; FOS Chemical sciences; Developmental Biology; Biological Sciences; Earth and Environmental Sciences; Evolutionary Biology; FOS Biological sciences; Molecular Biology; Cell Biology; Biochemistry; Biophysics; Crassostrea gigas; Pacific oyster
• Online Access: https://dx.doi.org/10.6084/m9.figshare.c.2067635.v1; https://tandf.figshare.com/collections/The_sperm_proteome_of_the_Pacific_oyster_i_Crassostrea_gigas_i_and_immunolocalization_of_heat_shock_proteins/2067635/1

The Pacific oyster Crassostrea gigas is a potential model organism of bivalve mollusks. Comprehensive studies on the proteome of its sperm are necessary to expand our understanding on its reproduction and development, which however are still poor currently. In this study, to improve the situation, we conducted a proteomic analysis on the sperm based on two-dimensional electrophoresis combined with protein identification through mass spectra data. Fifty-six protein spots with constant and relatively high expression levels were selected for protein identification. Among them, 36 were identified (corresponding to 31 proteins), including cytoskeletal proteins, proteins involved in energy supply, protein modifiers, signal regulators, antioxidant proteins, and others. We proposed that these proteins might play important roles in sperm motility, gamete interaction, and oxidation resistance. In particular, we observed several heat shock proteins that were proved to play essential roles in animal sperms. A further ...